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Journal ArticleDOI

Silver oxide nano-colloid based selective colorimetric sensor for the recognition of cystine in aqueous solution

06 Jun 2020-Materials Research Innovations (Taylor & Francis)-Vol. 24, Iss: 4, pp 202-209
TL;DR: Sodium alginate modified Ag2O (SA@Ag2O) nano-colloid is synthesized and studied colorimetric response towards eight selected amino acids in aqueous medium at room temperature as mentioned in this paper.
Abstract: Sodium alginate modified Ag2O (SA@Ag2O) nano-colloid is synthesised and studied colorimetric response towards eight selected amino acids in aqueous medium at room temperature. The synthesised SA@Ag...
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TL;DR: In this article , the selective interaction of amino acid d-cysteine (d-Cys) with citrate caped gold nanoparticles (Au NPs) in the presence of a fluorescent dye, rhodamine B (RhB), in aqueous solution was investigated.
Abstract: In this communication, we report the comparative and selective interaction of amino acid d-cysteine (d-Cys) with citrate caped gold nanoparticles (Au NPs) in the presence of a fluorescent dye, rhodamine B (RhB), in aqueous solution. Au NPs of size 27.5 nm could almost fully quench the steady-state fluorescence emission of RhB at their optimum concentrations in the mixed solution. The interactions of d-Cys, l-Cys, all other relevant d- and l-amino acids, neurotransmitters, and other relevant biological compounds with the Au NPs/RhB mixed solution have been explored by monitoring the fluorescence recovery efficiencies from the almost fully quenched state of RhB fluorescence via a simple steady-state spectrofluorometric method. The higher fluorescence recovery for the interaction of d-Cys with the Au NPs/RhB mixed system is accompanied by a distinct color change (red-wine to bluish-black) of the assay medium after the reaction compared to that of all other interfering compounds considered in this work. The sensitivity of this fluorometric response lies in a broad linear range of concentrations of d-Cys and the limit of detection (LOD) is found to be 4.2 nM, which is low compared to many other methods available in the literature. The different degrees of interaction of d-Cys and l-Cys with the Au NPs/RhB mixed sample have been further explored by circular dichroism (CD) spectroscopy and Fourier transform infrared (FTIR) spectroscopy. The selective interaction of d-Cys with the proposed Au NPs/RhB mixed system is also found to be correlated with interparticle cross-linking and aggregations of nanoparticles by the analysis of ζ potential and dynamic light scattering (DLS) study, transmission electron microscopy (TEM), atomic force microscopy (AFM), UV–vis absorption spectroscopy etc. The proposed interaction mechanism is further studied with a normal human urine sample to elucidate that the optimized combination of Au NPs and RhB may be realized as an efficient platform for detection of the amino acid d-Cys in a real biosample via a simple fluorometric approach.

1 citations

References
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Journal ArticleDOI
TL;DR: This electrode shows high selectivity for the response to cysteine, as compared with many common inorganic anions, salicylate, and other kinds of amino acids, and has a fast response time, micromolar detection limit, and good long-term stability.
Abstract: A novel cysteine-selective electrode based on lead phthalocyanine (PbPc) as ionophore is described. The electrode was prepared by incorporating PbPc into a plasticized poly(vinyl chloride) (PVC) membrane, which was directly coated on the surface of a graphite electrode. This electrode shows high selectivity for the response to cysteine, as compared with many common inorganic anions, salicylate, and other kinds of amino acids. The influence of membrane composition, pH, and the effect of lipophilic cationic and anionic additives on the response characteristics of the electrode were investigated. The resulting sensor demonstrates nernstian response over a wide linear range of cysteine concentration (1 × 10-6 to 5 × 10-2 M). The electrode has a fast response time, micromolar detection limit (∼1 × 10-6 M), and good long-term stability (more than 1 month). The prepared electrode was used for determination of cysteine in a synthetic human serum sample, and very good recovery results were obtained over a wide con...

958 citations


"Silver oxide nano-colloid based sel..." refers background in this paper

  • ...The deficiency of cysteine is associated with slow growth, hair depigmentation, liver damage, skin lesions, etc [3]....

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Journal ArticleDOI
TL;DR: A complete set of spectral information application to proteins in the whole mid infrared region could be described precise and the potential of the lower spectral region to study typical cofactor ligands like histidine, shown.

234 citations

Journal ArticleDOI
TL;DR: A method for the simultaneous measurement of two biologically important thiol compounds cysteine and homocysteines and five amino acids including neurotransmitters aspartate and glutamate is reported.

216 citations


"Silver oxide nano-colloid based sel..." refers background in this paper

  • ...For instance, high level of glutamic acid content in plasma leads to acute ischaemic stroke [1]....

    [...]

Journal ArticleDOI
Xin Wang1, Huifang Wu1, Qin Kuang1, Rong-Bin Huang1, Zhaoxiong Xie1, Lan-Sun Zheng1 
16 Feb 2010-Langmuir
TL;DR: It has been found the antibacterial activity of Ag(2)O particles against E. coli depends on the shape of Ag('s2')O particles, demonstrating that the surface structure of Ag("s2")O particles affects the antib bacterial activity.
Abstract: Ag2O particles with different polyhedral shapes including octahedron, truncated octahedron, and cube were successfully synthesized by a simple wet-chemical method using silver nitrate, ammonia, and sodium hydroxide as raw materials at room temperature. Simply by tuning the concentration of starting materials, the shape of Ag2O particles evolved from octahedron to cube, and the size gradually decreased from 1−2 μm to 400−700 nm. As examples for promising applications, the antibacterial activities of the as-prepared Ag2O polyhedral particles were preliminarily studied. It has been found the antibacterial activity of Ag2O particles against E. coli depends on the shape of Ag2O particles, demonstrating that the surface structure of Ag2O particles affects the antibacterial activity.

175 citations


"Silver oxide nano-colloid based sel..." refers background in this paper

  • ...Metal oxide colloids have been exploited as colorimetric sensor to detect Ag metal ion [23], halides [24], protein separator [25,26], cysteine and homocysteine detection [27], Phenylalanine detection [28],antioxidant activity [29], arsenic detection [30], etc....

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  • ...(a) UV-Vis spectrum (b) Powder XRD pattern (c) TEM image (d) particle size distribution of SA@Ag2O nano-colloid [25]....

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  • ...[25,26], the formation process of silver oxide can be viewed as follows....

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Journal ArticleDOI
TL;DR: The complexes of the silver ion Ag+ with twenty naturally occurring amino acids have been calculated using hybrid density functional theory at the B3LYP/DZVP level.
Abstract: The complexes of silver ion, Ag+, with the twenty naturally occurring amino acids have been calculated using hybrid density functional theory at the B3LYP/DZVP level. For all of these silver complexes, several possible structures were examined, but as there are remarkable similarities between all the structures at the global minima, only summarized data are reported. All of the complexes, except that with proline, are solvated ions. Amino acids containing only hydrocarbon side chains are bidentate, coordinating through the amino and carbonyl groups and the remaining amino acids (with the exception of proline) are tricoordinate with the same two interactions as in the simpler amino acids and an additional interaction through the side chain. The proline complex contains zwitterionic proline with the Ag+ ion attached to the carboxylate anion. Enthalpies (at 298 K) for dissociation of Ag+ from the complexes range from 49.3 kcal mol-1 for glycine to 80.4 kcal mol-1 for arginine. Free energies for these reactio...

140 citations