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Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids
Arnold J. Berk,Phillip A. Sharp +1 more
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A simple and sensitive method for detecting, sizing and mapping RNA transcripts from viral or cloned DNAs has been developed and used to examine the cytoplasmic transcripts produced during the early phase of adenovirus 2 (Ad2) infection of HeLa cells.About:
This article is published in Cell.The article was published on 1977-11-01. It has received 2367 citations till now. The article focuses on the topics: Restriction fragment & Restriction enzyme.read more
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Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter
TL;DR: In this paper, a simple and efficient method for synthesizing pure single stranded RNAs of virtually any structure is described, based on the unusually specific RNA synthesis by bacteriophage SP6 RNA polymerase which initiates transcription exclusively at an SP6 promoter.
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A conserved AU sequence from the 3′ untranslated region of GM-CSF mRNA mediates selective mRNA degradation
Gray D. Shaw,Robert Kamen +1 more
TL;DR: It is proposed that the AU sequences are the recognition signal for an mRNA processing pathway which specifically degrades the mRNAs for certain lymphokines, cytokines, and proto-oncogenes.
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Self-splicing RNA: Autoexcision and autocyclization of the ribosomal RNA intervening sequence of tetrahymena
Kelly Kruger,Paula J. Grabowski,Arthur J. Zaug,Julie Sands,Daniel E. Gottschling,Thomas R. Cech +5 more
TL;DR: It is proposed that the IVS portion of the RNA has several enzyme-like properties that enable it to break and reform phosphodiester bonds and that enzymes, small nuclear RNAs and folding of the pre-rRNA into an RNP are unnecessary for these reactions.
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Cell-Specific Regulation of the c-myc Gene by Lymphocyte Mitogens and Platelet-Derived Growth Factor
TL;DR: A regulatory linkage between the function of two oncogenes--c-myc and c-sis--the latter being the putative structural gene for PDGF is suggested, consistent with a model that a labile protein may regulate c- myc levels in these cells.
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Expression profiling using cdna microarrays
TL;DR: Technical aspects of cDNA microarrays are reviewed, including the general principles, fabrication of the arrays, target labelling, image analysis and data extraction, management and mining.
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Detection of specific sequences among DNA fragments separated by gel electrophoresis.
TL;DR: This paper describes a method of transferring fragments of DNA from agarose gels to cellulose nitrate filters that can be hybridized to radioactive RNA and hybrids detected by radioautography or fluorography.
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Detection of two restriction endonuclease activities in Haemophilus parainfluenzae using analytical agarose--ethidium bromide electrophoresis
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Analysis of restriction fragments of T7 DNA and determination of molecular weights by electrophoresis in neutral and alkaline gels.
TL;DR: Electrophoresis in alkaline gels can provide accurate molecular weights for linear, single-Stranded DNAs, and should be useful in analyzing DNA for single-strand breaks, depurinations or topological differences such as ring forms.
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Purification and further properties of single-strand-specific nuclease from Aspergillus oryzae.
TL;DR: The utility of this nuclease for measuring annealing of labeled DNA is discussed and the enzyme is shown to be absolutely specific for single-stranded nucleic acids.
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Dependence of the melting temperature of DNA on salt concentration
TL;DR: These calculations support the hypothesis that the change of Tm with salt concentration is due to changes in the screened interactions between the fixed phosphate charges, and indicate some of the limitations of the theoretical model.