Journal ArticleDOI
Social network architecture of human immune cells unveiled by quantitative proteomics
Jan C. Rieckmann,Roger Geiger,Roger Geiger,Daniel Hornburg,Tobias Wolf,Tobias Wolf,Ksenya Kveler,David Jarrossay,Federica Sallusto,Shai S. Shen-Orr,Antonio Lanzavecchia,Antonio Lanzavecchia,Matthias Mann,Felix Meissner +13 more
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TLDR
High-resolution mass-spectrometry-based proteomics revealed a specialization of immune cells for ligand and receptor expression, thereby connecting distinct immune functions and discovering fundamental intercellular communication structures and previously unknown connections between cell types.Abstract:
The immune system is unique in its dynamic interplay between numerous cell types. However, a system-wide view of how immune cells communicate to protect against disease has not yet been established. We applied high-resolution mass-spectrometry-based proteomics to characterize 28 primary human hematopoietic cell populations in steady and activated states at a depth of >10,000 proteins in total. Protein copy numbers revealed a specialization of immune cells for ligand and receptor expression, thereby connecting distinct immune functions. By integrating total and secreted proteomes, we discovered fundamental intercellular communication structures and previously unknown connections between cell types. Our publicly accessible (http://www.immprot.org/) proteomic resource provides a framework for the orchestration of cellular interplay and a reference for altered communication associated with pathology.read more
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CD4 + T cell help in cancer immunology and immunotherapy
TL;DR: This Review highlights the cellular dynamics and membrane receptors that mediate CD4+ T cell help and the molecular mechanisms of the enhanced antitumour activity of CTLs and discusses the molecular nature of help signals and how they can be harnessed to improve cancer immunotherapy.
Journal ArticleDOI
The gasdermins, a protein family executing cell death and inflammation
TL;DR: This Review provides a comprehensive overview of the gasdermin family, the mechanisms that control their activation and their role in inflammatory disorders and cancer.
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Deciphering cell-cell interactions and communication from gene expression.
TL;DR: This Review highlights discoveries enabled by analyses of cell–cell interactions from transcriptomic data and reviews the methods and tools used in this context.
Journal ArticleDOI
ImmPort, toward repurposing of open access immunological assay data for translational and clinical research.
Sanchita Bhattacharya,Patrick Dunn,Cristel G. Thomas,Barry Smith,Henry Schaefer,Jieming Chen,Zicheng Hu,Kelly A. Zalocusky,Ravi D. Shankar,Shai S. Shen-Orr,Elizabeth Thomson,Jeffrey Wiser,Atul J. Butte +12 more
TL;DR: The ImmPort data repository was created for the broader research community to explore the wide spectrum of clinical and basic research data and associated findings and allows research data to be repurposed to accelerate the translation of new insights into discoveries.
Journal ArticleDOI
histoCAT: analysis of cell phenotypes and interactions in multiplex image cytometry data.
Denis Schapiro,Hartland W. Jackson,Swetha Raghuraman,Jana R. Fischer,Vito R T Zanotelli,Daniel Schulz,Charlotte Giesen,Raul Catena,Zsuzsanna Varga,Bernd Bodenmiller +9 more
TL;DR: This work has developed an open-source, computational histology topography cytometry analysis toolbox (histoCAT) to enable interactive, quantitative, and comprehensive exploration of individual cell phenotypes, cell–cell interactions, microenvironments, and morphological structures within intact tissues.
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