Journal ArticleDOI
Somatic embryogenesis from cultured leaf segments of Zea mays.
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Basal leaf segments of 3 to 4 week old maize seedlings plated on SH medium with 30 μM dicamba produced embryogenic callus and/or somatic embryos that germinated and the resulting seedlings could be established in culture tubes.Abstract:
Basal leaf segments of 3 to 4 week old maize (Zea mays L.) seedlings plated on SH medium with 30 μM dicamba produced embryogenic callus and/or somatic embryos. Histological evidence showed that some of the embryos arose directly from the explant. When leaf segments with embryos were transferred to MS medium with 1.0 μM NAA, 1.0 μM IAA, 2.0 μM 2iP, and 60 g/l sucrose, the embryos germinated and the resulting seedlings could be established in culture tubes. These responses were obtained from three inbred lines, CHI31, S615, and S7.read more
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Inbred corn plant 89AHD12 and seeds thereof
TL;DR: In this article, the authors provided an inbred corn plant designated 89AHD12, which was used to produce corn seeds and plants produced by crossing the inbred plant with another corn plant, such as another inbred, and to crosses with related species.
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Inbred corn line LH283BtMON810
TL;DR: An inbred corn line, designated LH283BtMON810, is disclosed in this paper, which relates to the seeds of the inbred line and to the plants of the line.
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Inbred corn line PHHB9
TL;DR: In this paper, the authors provided an inbred corn line, designated PHHB9, and a method for producing a corn plant produced by crossing the inbred line with itself or with another corn plant.
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Inbred maize line PH0HC
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Journal ArticleDOI
Morphogenic Regulators Baby boom and Wuschel Improve Monocot Transformation
Keith S. Lowe,Emily Wu,Ning Wang,George J. Hoerster,Craig Hastings,Myeong-Je Cho,Christopher J. Scelonge,Brian Lenderts,Mark A. Chamberlin,Josh Cushatt,Lijuan Wang,Larisa Ryan,Tanveer Khan,Julia Chow-Yiu,Wei Hua,Maryanne Yu,Jenny Banh,Zhongmeng Bao,Kent Brink,Elizabeth Igo,Bhojaraja Rudrappa,PM Shamseer,Wes Bruce,Lisa J. Newman,Bo Shen,Peizhong Zheng,Dennis L. Bidney,Carl Falco,Jim Register,Zuo-Yu Zhao,Deping Xu,Todd J. Jones,William J. Gordon-Kamm +32 more
TL;DR: A transformation approach involving overexpression of the maize (Zea mays) Baby boom (Bbm) and maize Wuschel2 (Wus2) genes, which produced high transformation frequencies in numerous previously nontransformable maize inbred lines is reported.
References
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Journal ArticleDOI
A revised medium for rapid growth and bio assays with tobacco tissue cultures
Toshio Murashige,Folke Skoog +1 more
TL;DR: In vivo redox biosensing resolves the spatiotemporal dynamics of compartmental responses to local ROS generation and provide a basis for understanding how compartment-specific redox dynamics may operate in retrograde signaling and stress 67 acclimation in plants.
Journal ArticleDOI
A simple and general method for transferring genes into plants
Robert B. Horsch,J. E. Fry,Nancy L. Hoffmann,Marco Wallroth,David Alan Eichholtz,Stephen G. Rogers,Robert T. Fraley +6 more
TL;DR: This method for producing transformed plants combines gene transfer, plant regeneration, and effective selection for transformants into a single process and should be applicable to plant species that can be infected by Agrobacterium and regenerated from leaf explants.
Journal ArticleDOI
Somaclonal variation — a novel source of variability from cell cultures for plant improvement
Philip J. Larkin,W. R. Scowcroft +1 more
TL;DR: It is argued that this variation in plant cell culture itself generates genetic variability (somaclonal variation) that may be employed to enhance the exchange required in sexual hybrids for the introgression of desirable alien genes into a crop species.
Journal ArticleDOI
Medium and techniques for induction and growth of monocotyledonous and dicotyledonous plant cell cultures
Roy U. Schenk,A. C. Hildebrandt +1 more
TL;DR: The composition of a medium is described that proved useful to culture callus of a variety of monocotyledonous and dicotylingonous plants and facilitated work with single cells and the enzymatic removal of cell walls in related studies.
Journal ArticleDOI
Establishment and maintenance of friable, embryogenic maize callus and the involvement of L-proline.
Charles L. Armstrong,C. E. Green +1 more
TL;DR: Frequentencies of friable-callus initiation and somatic-embryoid formation increased linearly with addition to N6 medium, and L-Glutamine was not a satisfactory substitute for L-proline.