Some changes in biochemical composition with season and during the moulting cycle of the common shore crab, Carcinus maenas (L.)
01 Nov 1970-Journal of Experimental Marine Biology and Ecology (Elsevier)-Vol. 5, Iss: 3, pp 199-233
TL;DR: Changes in the size and biochemical composition (glycogen, total carbohydrate, neutral fat, phospholipids, protein and non-protein nitrogen) have been followed throughout the year in the ovary and both male and female hepatopancreas of Carcinus maenas.
About: This article is published in Journal of Experimental Marine Biology and Ecology.The article was published on 1970-11-01. It has received 127 citations till now. The article focuses on the topics: Carcinus & Carcinus maenas.
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TL;DR: The phosphosulphovanillin method, previously used only for blood serum samples, is recommended for estimation of ‘total’ lipids in the tissue of marine animals, based on using a cholesterol standard which has previously been related to gravimetric values obtained on blood sera.
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TL;DR: It is proposed that TAG content can be correlated with larval condition when expressed in the form of a TAG-sterol ratio that accounts for the size dependency of TAG content.
Abstract: The lipid biochemistry of environmentally stressed larval fish, bivalves and crustaceans is presented to illustrate the utility of a larval condition index based on lipid composition. Larvae under ...
244 citations
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TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
59,550 citations
18,029 citations
TL;DR: Evidence for the existence or formation of at least two water-insoluble glycogen complexes in liver was presented and losses of glycogen in the ethanol precipitation step were found to be preventable by the use of a coprecipitant.
517 citations
01 Jan 1946
TL;DR: This book has been designed to give the graduate JL student a working description of the methods used as a routine in the study of tissue metabolism and only methods which are likely to need the minimum equipment found normally in a biological laboratory such as a respirometer, colorimeter and centrifuge are described.
Abstract: THIS book has been designed to give the graduate JL student a working description of the methods used as a routine in the study of tissue metabolism. Only methods which are likely to need the minimum equipment found normally in a biological laboratory such as a respirometer, colorimeter and centrifuge are described. In addition to details of manometric, Thunberg tube and related electrometric techniques, much information is given on the preparation of physiologically important metabolites and particularly on the preparation and estimation of the phos-phorylated carbohydrate intermediates. Methods are given for preparing animal, plant and microbial tissues and the homogenate technique is described. The wider use cf manometric methods for the estimation of particular metabolites, which with specific enzyme systems utilize or produce gases, such as the estimation of urea with urease and of succinic acid and xanthine by their oxidases, is advocated. A very extensive bibliography follows each of the sixteen chapters.Manometric Techniques and Related Methods for the Study of Tissue MetabolismW. W.UmbreitR. H. BurrisJ. F.StaufferBy. Chapters on Specialized Techniques by P. P. Cohen, G. A. LePage and V. R. Potter. Pp. vii + 198. (Minneapolis, Minn.: Burgess Publishing Company, 1945.) 3.50 dollars.
516 citations