Specific binding of leucyl transfer rna to an immature form of l-threonine deaminase: its implications in repression
01 Aug 1970-Proceedings of the National Academy of Sciences of the United States of America (National Academy of Sciences)-Vol. 66, Iss: 4, pp 1027-1035
TL;DR: The in vitro assembly of L-threonine deaminase from its constituent parts results in the formation of an inactive species of this enzyme which specifically and reversibly binds leucyl-tRNA.
Abstract: The in vitro assembly of L-threonine deaminase from its constituent parts results in the formation of an inactive species of this enzyme which specifically and reversibly binds leucyl-tRNA. The native, catalytically active enzyme, which is derived from this species in the presence of maturation-inducing ligands, does not bind tRNA. The possible involvement of this protein-tRNA complex in the repression of the ilv (ADE) operon is discussed.
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