Specificity of cycloheximide in higher plant systems.
R. J. Ellis,I. R. MacDonald +1 more
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TLDR
It is concluded that cycloheximide can affect cellular metabolism other than by inhibiting protein synthesis and that the inhibition of ion uptake may be due to disruption of the energy supply.Abstract:
Although cycloheximide is extremely inhibitory to protein synthesis in vivo in higher plants, the reported insensitivity of some plant ribosomes suggests that it may not invariably act at the ribosomal level. This suggestion is reinforced by results obtained with red beet storage tissue disks, the respiration of which is stimulated by cycloheximide at 1 microgram per milliliter. Inorganic ion uptake by these disks is inhibited by cycloheximide at 1 microgram per milliliter while the uptake of organic compounds, by comparison, is unaffected. Ion uptake by all nongreen tissues tested is inhibited by cycloheximide, but leaf tissue is unaffected, indicating that the ion absorption mechanism in the leaf may differ fundamentally from that in the root. It is concluded that cycloheximide can affect cellular metabolism other than by inhibiting protein synthesis and that the inhibition of ion uptake may be due to disruption of the energy supply.read more
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Experiments and hypothesis concerning the primary action of auxin in elongation growth
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The Role of Protein Synthesis in the Senescence of Leaves: I. The Formation of Protease
Colin Martin,Kenneth V. Thimann +1 more
TL;DR: The senescence of oat leaves has been studied by following the loss of chlorophyll and protein and the increase of alpha-amino nitrogen, after detachment and darkening, and shown to be a sequential one in which protein synthesis, most probably the formation of a proteolytic enzyme with l-serine in its active center, is of prime importance.
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An early response to gibberellic Acid not requiring protein synthesis.
Yosef Ben-Tal,Joseph E. Varner +1 more
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References
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Journal ArticleDOI
Site of action of cycloheximide in cells of Saccharomyces pastorianus. 3. Further studies on the mechanism of action and the mechanism of resistance in saccharomyces species.
Malcolm R. Siegel,Hugh D. Sisler +1 more
TL;DR: Kinetics of cycloheximide-inhibited transfer of amino acids from soluble RNA to protein in a system from Saccharomyces pastorianus was further investigated and showed that resistance or susceptibility of the system to cyclo heximide is determined by the ribosomes and not by the supernatant enzymes.
Journal ArticleDOI
Chloroplast Ribosomes: Stereospecificity of Inhibition by Chloramphenicol
TL;DR: Results remove doubts raised by nonstereospecific effects of chloramphenicol on higher plant cells and support the concept that chloroplasts have evolved from prokaryotes.
Journal ArticleDOI
Modified ribosomes conferring resistance to cycloheximide in mutants of Saccharomyces cerevisiae.
Journal ArticleDOI
Pentose Phosphate Pathway as a Major Component of Induced Respiration of Carrot and Potato Slices.
T A Rees,Harry Beevers +1 more
TL;DR: Examination of the pathways of glucose oxidation in washed tissue presents evidence that the development of the induced respiration was accompanied by an increase in the proportion of glucose oxidized through the PP pathway.
Journal ArticleDOI
Activation of protein synthesis by microsomes from aging beet disks.
R. J. Ellis,I. R. MacDonald +1 more
TL;DR: The results support the view that the aging phenomenon involves a derepression of gene activity in microsomal fractions of red beetroot.
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