Journal ArticleDOI
Specificity, stereochemistry, and mechanism of the color reaction between p-dimethylaminocinnamalhyde and biotin analogs.
Reads0
Chats0
TLDR
Biotin and other cyclic ureido compounds monoalkylated on each nitrogen have been shown to react with p-dimethylaminocinamaldehyde in anhydrous solution or on dry chromatograms in a classic way for formation of the conjugate imine.About:
This article is published in Analytical Biochemistry.The article was published on 1970-03-01. It has received 83 citations till now. The article focuses on the topics: Imine & Biotin.read more
Citations
More filters
Journal ArticleDOI
Enzymatic synthesis of biotin-labeled polynucleotides: novel nucleic acid affinity probes
TL;DR: Biotin-labeled polynucleotides, both single and double-stranded, are selectively and quantitatively retained on avidin-Sepharose, even after extensive washing with 8 M urea, 6 M guanidine hydrochloride, or 99% formamide.
Journal ArticleDOI
Non-radioactive hybridization probes prepared by the chemical labelling of DNA and RNA with a novel reagent, photobiotin
TL;DR: The sensitivity of detection of target RNA in dot-blots and Northern blots was equivalent to that obtained with 32p-labelled DNA probes, and photobiotin was also used for the labelling of proteins with biotin.
Journal ArticleDOI
Polyacrylamides Bearing Pendant α-Sialoside Groups Strongly Inhibit Agglutination of Erythrocytes by Influenza Virus: The Strong Inhibition Reflects Enhanced Binding through Cooperative Polyvalent Interactions
TL;DR: An ELISA assay is described for measuring the binding of influenza virus A-X31 to α-sialoside groups that are linked to biotin-labeled polyacrylamides, and the role of steric stabilization in the mechanism is shown to be directly related to the binding affinity of the polymers for the viral surface.
Journal ArticleDOI
Acetyl Coenzyme A Carboxylase System of Escherichia coli PURIFICATION AND PROPERTIES OF THE BIOTIN CARBOXYLASE, CARBOXYLTRANSFERASE, AND CARBOXYL CARRIER PROTEIN COMPONENTS
TL;DR: The two catalytic components of the E. coli acetyl-CoA carboxylase system are devoid of free or covalently bound biotin yet have the ability to carry out their respective model half-reactions utilizing free d-biotin derivatives in place of carboxy carrier protein.
Journal ArticleDOI
Cleavable biotin probes for labeling of biomolecules via azide-alkyne cycloaddition.
Janek Szychowski,Alborz Mahdavi,Jennifer J. L. Hodas,John D. Bagert,John T. Ngo,Peter Landgraf,Daniela C. Dieterich,Erin M. Schuman,David A. Tirrell +8 more
TL;DR: Five cleavable biotin probes are described for use in labeling of proteins and other biomolecules via azide-alkyne cycloaddition and a probe constructed around a dialkoxydiphenylsilane (DADPS) linker was found to be cleaved efficiently when treated with 10% HCO(2)H for 0.5 h.
References
More filters
Journal ArticleDOI
Metal ion complexes with biotin and biotin derivatives. Participation of sulfur in the orientation of divalent cations
Journal ArticleDOI
Bacterial degradation of biotin. Catabolism of 14C-biotin and its sulfoxides.
TL;DR: A biotin-degrading particulate system has been obtained by sonic rupture of a soil pseudomonad which grows on d-biotin as sole source of carbon, nitrogen, and sulfur and is efficiently catabolized by the particulate preparations and exhibit mutual competition as substrates.
Journal ArticleDOI
Bacterial Degradation of Biotin III. METABOLISM OF 14C-CARBONYL-LABELED BIOTIN
TL;DR: These findings establish that the side chain of biotin is degraded by β-oxidation with loss of 2 acetate units, and that after the complete degradation of the side Chain, oxidation of both carbons bridging the ureido to thiophane rings occurs, producing urea which is degraded to CO2 and NH3 by urease.
Journal ArticleDOI
Possible Biochemical Implications of the Crystal Structure of Biotin
TL;DR: An examination of the molecular architecture of biotin, as determined by x-ray crystallographic analysis, has indicated that biotin may be capable of forming an intramolecular hydrogen bond in solution.
Journal ArticleDOI
Bacterial Degradation of Biotin II. CATABOLISM OF 14C-HOMOBIOTIN AND 14C-NORBIOTIN
TL;DR: Findings indicate the presence of a β oxidative system which catalyzes the degradation of the side chain of biotin as well as of several biotin analogues.