Stable genetic transformation of Vigna mungo L. Hepper via Agrobacterium tumefaciens
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...We therefore opted for regeneration via organogenesis, which has been a successful platform for transformation protocols in other plants, including legumes (Schroeder et al. 1993; Olhoft and Somers 2001; Saini et al. 2003; Sarmah et al. 2004)....
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"Stable genetic transformation of Vi..." refers methods in this paper
...…washed four to five times with sterile distilled water, then soaked in sterile distilled water in a conical flask (250 ml) plugged with non- absorbent cotton wrapped in cheese cloth for 16 h or cultured on MS (Murashige and Skoog 1962) basal medium (MSB) for 4 days at 25€2 C under light conditions....
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...They were subsequently washed four to five times with sterile distilled water, then soaked in sterile distilled water in a conical flask (250 ml) plugged with non- absorbent cotton wrapped in cheese cloth for 16 h or cultured on MS (Murashige and Skoog 1962) basal medium (MSB) for 4 days at 25€2 C under light conditions....
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9,765 citations
"Stable genetic transformation of Vi..." refers methods in this paper
...Enzyme assay The GUS activity in tissues was determined by a histochemical assay according to Jefferson et al. (1987)....
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9,342 citations
"Stable genetic transformation of Vi..." refers methods in this paper
...The explants were cultured in a vertically upright position with the hypocotyl cut end slightly embedded in medium containing MS salts, B5 vitamins (Gamborg et al.1968), 3% (w/v) sucrose (MSB) and different concentrations of BA (0.5–10 M), pH 5.8, solidified with 0.7% agar (Hi Media, Mumbai)....
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