Stable isotope labeling by amino acids in cell culture, SILAC, as a simple and accurate approach to expression proteomics.
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...or by mixing SILAC-labeled protein mixtures before separatio...
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...Other elegant approaches use cell-culture enrichment with a stable isotope-labeled amino acid, including arginine (5), lysine (6), tyrosine (7), and leucine (8), for in vivo incorporation of a mass difference to support relative quantitation....
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References
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"Stable isotope labeling by amino ac..." refers background in this paper
...Proteomics, the large scale study of the protein complement of a cell or tissue, has its origins in the technology of two-dimensional (2D)(1) gel electrophoresis invented more than 25 years ago (1, 2)....
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"Stable isotope labeling by amino ac..." refers methods in this paper
...trospray experiments (30), digests were desalted and concentrated on a microcolumn packed into GELoader tips (31)....
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