Journal ArticleDOI
Statistical analyses for in vitro cytogenetic assays using Chinese hamster ovary cells
B. H. Margolin,Michael A. Resnick,J. Y. Rimpo,P. Archer,Sheila M. Galloway,A. D. Bloom,Errol Zeiger +6 more
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In this paper, a trend test for evidence of a dose response is proposed for such SCE data, where the percent of cells with chromosome aberrations is the response of interest, and Monte Carlo methods are used to show that the trend test is more sensitive than four other statistical procedures considered for the analysis of Poisson-distributed SCE.Abstract:
It is a widely held view that objective statistical criteria are needed for the evaluation of genetic toxicity assays. This paper presents statistical methods for the analysis of data from in vitro sister chromatid exchange (SCE) and chromosome aberration tests that use Chinese hamster ovary cells. For SCEs, an extensive study of solvent control results demonstrated that there is a substantial interday component of variability in the data, and that a Poisson sampling model is applicable to data generated via the protocol of Galloway et al [1985]. Consequently, a trend test for evidence of a dose response is proposed for such SCE data. As an illustration of this statistical method, analysis of data previously considered to be negative [Gulati et al, 1985] indicates that di(2-ethyl-hexyl) phthalate induces a weak, but reproducible, SCE dose response in CHO cells. Monte Carlo methods are used to show that the trend test is more sensitive than four other statistical procedures considered for the analysis of Poisson-distributed SCEs. A similar trend test for dose response in proportions is proposed for chromosome aberration data, where the percent of cells with chromosome aberrations is the response of interest. Sensitivity (or power) studies indicate that three doses and a control with 50 cells/dose point is a reasonable design for an in vitro SCE study that uses the Galloway et al protocol. For in vitro chromosome aberrations, however, three doses and a control with 100 cells/dose point appears to produce too insensitive an assay; an increase to 200 cells/dose point in the Galloway et al protocol seems worthy of serious consideration.read more
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Journal ArticleDOI
Prediction of chemical carcinogenicity in rodents from in vitro genetic toxicity assays.
Raymond W. Tennant,Barry H. Margolin,Michael D. Shelby,Errol Zeiger,Joseph K. Haseman,Judson W. Spalding,William J. Caspary,Michael A. Resnick,Stanley Stasiewicz,Beth Anderson,Robert Minor +10 more
TL;DR: Four widely used in vitro assays for genetic toxicity were evaluated for their ability to predict the carcinogenicity of selected chemicals in rodents, indicating that chemicals positive in one in vitro assay tended to be positive in the other in vitro Assays.
Journal ArticleDOI
Handbook of Statistical Tables.
D. G. Beech,D. B. Owen +1 more
TL;DR: The Handbook of Statistical Tables (HNT) as mentioned in this paper is a collection of tables from B.D. Owen's "Handbook of Statistical Table Tables" (1962).
Journal ArticleDOI
Chromosome aberrations and sister chromatid exchanges in chinese hamster ovary cells: Evaluations of 108 chemicals
Sheila M. Galloway,M. J. Armstrong,C. Reuben,S. Colman,B. M. Brown,C. Cannon,A. D. Bloom,F. Nakamura,M. Ahmed,S. Duk,J. Rimpo,Barry H. Margolin,Michael A. Resnick,Beth Anderson,Errol Zeiger +14 more
TL;DR: Results from the testing of 108 coded chemicals in Chinese hamster ovary cells for the induction of chromosome aberrations and sister chromatid exchanges (SCEs) are presented.
Journal ArticleDOI
Mammalian in vivo cytogenetic assays. Analysis of chromosome aberrations in bone marrow cells.
Journal ArticleDOI
The in vivo micronucleus assay in mammalian bone marrow and peripheral blood. A report of the U.S. Environmental Protection Agency Gene-Tox Program
TL;DR: The protocol recommended for the micronucleus assay in mammalian bone marrow has been revised and simplified and the minimum number of cells to be scored per treatment group has been increased to 20,000 to increase the ability of the assay to detect a doubling of the control micron nucleus frequency.
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