Statistical optimization of medium components for improved synthesis of pectinase by Aspergillus niger
TL;DR: In this article, the synthesis of pectinase using Aspergillus niger, NCIM 548, has been enhanced by optimizing the carbon and nitrogen sources present in the medium.
Abstract: The synthesis of pectinase using Aspergillus niger, NCIM 548, has been enhanced by optimizing the carbon and nitrogen sources present in the medium. Enzyme synthesis was about 40% more using the optimized medium than using the unoptimized medium. The study provided information on the appropriate carbon source as well as the optimum ratio of carbon to nitrogen, yielding the highest enzyme levels.
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TL;DR: This short review highlights progress on purification and understanding the biochemical aspects of microbial pectinases.
305 citations
TL;DR: Many techniques are available in the fermentation medium designer’s toolbox (borrowing, component swapping, biological mimicry, one-at-a-time, statistical and mathematical techniques—experimental design and optimization), and considerable advantage can be gained by logical application of the techniques, combined with good experimental design.
Abstract: Many techniques are available in the fermentation medium designer’s toolbox (borrowing, component swapping, biological mimicry, one-at-a-time, statistical and mathematical techniques—experimental design and optimization, artificial neural networks, fuzzy logic, genetic algorithms, continuous fermentation, pulsed batch and stoichiometric analysis). Each technique has advantages and disadvantages, and situations where they are best applied. No one ‘magic bullet’ technique exists for all situations. However, considerable advantage can be gained by logical application of the techniques, combined with good experimental design.
289 citations
Cites methods from "Statistical optimization of medium ..."
...To estimate the quadratic polynomial requires a factorial design such as a full factorial with at least three levels per factor [119], eg a central composite [85, 103 ] or a Box‐Behnken design....
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...Nair & Panda [ 103 ] 1997 3 CCD SP Stat-ease 40% Lee & Chen [85] 1997 2 CCD SP SAS 38% Smith et al [134] 1997 4 2...
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...I partial factorial [21,26,32,37,57,59,80,88] 8 I central composite [26,34,39,55,85, 103 ,125,152] 8 I Plackett and Burman [2,22] 2 I orthogonal arrays/Latin rectangles [130,160] 2 I Box‐Behnken [125,150] 2 Optimization techniques I steepest ascent [15,57,88,124] 4 I multiple regression/response surface methodology 6 [55,85,119,123,153,162]...
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TL;DR: Using a genetic algorithm, it has been experimentally verified that process improvements can be achieved both for microbial and enzymatic conversions and for cell cultures despite the large number of medium components under simultaneous consideration, in exploring a new variable space.
220 citations
TL;DR: The results demonstrated that this SPB1 biosurfactant exhibited an important antimicrobial activity against microorganisms with multidrug-resistant profiles, and was very effective against Staphylococcus aureus, Staphyllococcus xylosus, Enterococcus faecalis, Klebsiella pneumonia, and so forth.
Abstract: During the last years, several applications of biosurfactants with medical purposes have been reported. Biosurfactants are considered relevant molecules for applications in combating many diseases. However, their use is currently extremely limited due to their high cost in relation to that of chemical surfactants. Use of inexpensive substrates can drastically decrease its production cost. Here, twelve solid substrates were screened for the production of Bacillus subtilis SPB1 biosurfactant and the maximum yield was found with millet. A Plackett-Burman design was then used to evaluate the effects of five variables (temperature, moisture, initial pH, inoculum age, and inoculum size). Statistical analyses showed that temperature, inoculum age, and moisture content had significantly positive effect on SPB1 biosurfactant production. Their values were further optimized using a central composite design and a response surface methodology. The optimal conditions of temperature, inoculum age, and moisture content obtained under the conditions of study were 37°C, 14 h, and 88%, respectively. The evaluation of the antimicrobial activity of this compound was carried out against 11 bacteria and 8 fungi. The results demonstrated that this biosurfactant exhibited an important antimicrobial activity against microorganisms with multidrug-resistant profiles. Its activity was very effective against Staphylococcus aureus, Staphylococcus xylosus, Enterococcus faecalis, Klebsiella pneumonia, and so forth.
108 citations
Cites methods from "Statistical optimization of medium ..."
...The statistical significance of the model was determined by the application of Fisher’s F test [27]....
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TL;DR: The addition of protein doubled the half-life times of partially purified PMG, PG I and PG II and the interaction effect of pectolytic enzymes on deactivation was found to be significant.
108 citations
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"Statistical optimization of medium ..." refers methods in this paper
...Maximization of the regression equation was carried out using an iterative procedure [ 16 ]....
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TL;DR: Results indicate that regulatory phenomena, such as induction-repression or activation-inhibition, related to pectinase synthesis by A. niger CH4 are different in the two types of fermentation.
Abstract: A study was made to compare the production of pectinase by Aspergillus niger CH4 in solid-state (SSF) and submerged (SmF) fermentations. Production of endo- (endo-p) and exo-pectinase (exo-p) by SSF was not reduced when glucose, sucrose or galacturonic acid (up to 10%) were added to a culture medium containing pectin. Moreover, both activities increased when concentrations of the carbon sources were also increased. In SmF, these activities were strongly decreased when glucose or sucrose (3%) was added to culture medium containing pectin. The addition of galacturonic acid affected endo-p activity production to a lesser extend than exo-p. Final endo-p and exo-p activities in SSF were three and 11 times higher, respectively, than those obtained in SmF. The overall productivities of SSF were 18.8 and 4.9 times higher for endo-p and exo-p, respectively, than those in SmF. These results indicate that regulatory phenomena, such as induction-repression or activation-inhibition, related to pectinase synthesis by A. niger CH4 are different in the two types of fermentation.
218 citations
01 Jan 1981
TL;DR: Enzymes are currently used in fruit and vegetable juice production as mentioned in this paper to obtain partial or total liquefaction of the fruit flesh for the production of fruit pulps and nectars, and to increase juice yield and ameliorate extraction of other fruit components such as color and flavour.
Abstract: Enzymes are currently used in fruit (and vegetable) juice production
(a)
for mash treatment
(i)
to obtain a partial or total liquefaction of the fruit flesh for the production of fruit pulps and nectars,
(ii)
to increase juice yield and ameliorate extraction of other fruit components such as colour and flavour;
(b)
for juice treatment
(i)
to reduce its viscosity and enable concentration,
(ii)
for better clarification, filtration and stabilisation.
79 citations
Journal Article•
54 citations