Status of Large-scale Analysis of Post-translational Modifications by Mass Spectrometry
Jesper V. Olsen,Matthias Mann +1 more
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TLDR
In this overview for the special PTM issue of Molecular and Cellular Proteomics, it is taken stock of where MS-based proteomics stands in the large-scale analysis of protein modifications.About:
This article is published in Molecular & Cellular Proteomics.The article was published on 2013-12-01 and is currently open access. It has received 510 citations till now.read more
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Ultradeep human phosphoproteome reveals a distinct regulatory nature of Tyr and Ser/Thr-based signaling.
Kirti Sharma,Rochelle C.J. D’Souza,Stefka Tyanova,Christoph Schaab,Jacek R. Wiśniewski,Jürgen Cox,Matthias Mann +6 more
TL;DR: A stringent experimental and computational workflow, capable of mapping more than 50,000 distinct phosphorylated peptides in a single human cancer cell line, is developed, suggesting that P-Tyr should be considered a functionally separate PTM of eukaryotic proteomes.
Journal ArticleDOI
Signaling Mechanisms in Pattern-Triggered Immunity (PTI)
TL;DR: In this article, the authors discuss the complex network of signaling pathways occurring during PTI, focusing on the involvement of mitogen-activated protein kinases, and discuss the role of the kinases in the defense against pathogen attacks.
Journal ArticleDOI
Metabolic regulation of gene expression through histone acylations
TL;DR: A model is proposed to explain the present understanding of how differential histone acylation is regulated by the metabolism of the different acyl-CoA forms, which in turn modulates the regulation of gene expression.
Journal ArticleDOI
The world of protein acetylation
TL;DR: This review gives a general overview of protein acetylation and the respective acetyltransferases, and focuses on the regulation of metabolic processes and physiological consequences that come along with proteinacetylation.
Journal ArticleDOI
Uncovering global SUMOylation signaling networks in a site-specific manner
Ivo A. Hendriks,Rochelle C.J. D’Souza,Bing Yang,Matty Verlaan-de Vries,Matthias Mann,Alfred C.O. Vertegaal +5 more
TL;DR: This work quantitatively studied SUMOylation dynamics in response to SUMO protease inhibition, proteasome inhibition and heat shock, and identified 70 phosphorylation and four acetylation events in proximity to SUMoylation sites, and provides evidence for acetylated-dependent SUMOolation of endogenous histone H3.
References
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Shao En Ong,Blagoy Blagoev,Irina Kratchmarova,Dan B. Kristensen,Hanno Steen,Akhilesh Pandey,Matthias Mann +6 more
TL;DR: SILAC is a simple, inexpensive, and accurate procedure that can be used as a quantitative proteomic approach in any cell culture system and is applied to the relative quantitation of changes in protein expression during the process of muscle cell differentiation.
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Multiplexed Protein Quantitation in Saccharomyces cerevisiae Using Amine-reactive Isobaric Tagging Reagents
Philip L. Ross,Yulin N. Huang,Jason Marchese,Brian L. Williamson,Kenneth C. Parker,Stephen J. Hattan,Nikita Khainovski,Sasi Pillai,Subhakar Dey,Scott Daniels,Subhasish Purkayastha,Peter Juhasz,Stephen A. Martin,Michael Bartlet-Jones,Feng He,Allan Jacobson,Darryl J. Pappin,Darryl J. Pappin +17 more
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STRING v9.1: protein-protein interaction networks, with increased coverage and integration
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Global, in vivo, and site-specific phosphorylation dynamics in signaling networks.
Jesper V. Olsen,Blagoy Blagoev,Florian Gnad,Boris Macek,Boris Macek,Chanchal Kumar,Peter Mortensen,Matthias Mann +7 more
TL;DR: A general mass spectrometric technology is developed and applied for identification and quantitation of phosphorylation sites as a function of stimulus, time, and subcellular location to provide a missing link in a global, integrative view of cellular regulation.
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