Journal ArticleDOI
Stochastic Collision Electrochemistry from Single G-Quadruplex/Hemin: Electrochemical Amplification and MicroRNA Sensing.
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TLDR
In this article, an electrochemical amplification strategy was developed to monitor the electrocatalytic behavior of single G-quadruplex/hemin (GQH) for the reaction between hydrogen peroxide and hydroquinone (HQ) through the collision upon a gold nanoelectrode.Abstract:
Stochastic collision electrochemistry is a hot topic in single molecule/particle research, which provides an opportunity to investigate the details of the single molecule/particle reaction mechanism that is always masked in ensemble-averaged measurements. In this work, we develop an electrochemical amplification strategy to monitor the electrocatalytic behavior of single G-quadruplex/hemin (GQH) for the reaction between hydrogen peroxide and hydroquinone (HQ) through the collision upon a gold nanoelectrode. The intrinsic peroxidase activities of single GQH were investigated by stochastic collision electrochemical measurements, giving further insights into understanding biocatalytic processes. Based on the unique catalytic activity of GQH, we have also designed a hybridization chain reaction strategy to detect miRNA-15 with good selectivity and sensitivity. This work provided a meaningful strategy to investigate the electrochemical amplification and the broad application for nucleic acid sensing at the single molecule/particle level.read more
Citations
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Nanozyme Catalytic Turnover and Self-Limited Reactions.
Mohamad Zandieh,Juewen Liu +1 more
TL;DR: In this paper, the surface atoms were considered as nanozyme active sites, and then the turnover numbers and rates were recalculated based on surface atoms, and the catalytic turnover of peroxidase Fe3O4 NPs was validated.
Journal ArticleDOI
Recent Progress in DNA Hybridization Chain Reaction Strategies for Amplified Biosensing.
TL;DR: In this article, the authors summarize the assembly principles of conventional HCR and some novel forms of linear/nonlinear HCR, and a deep discussion is provided about the challenges and future tasks of this field.
Journal ArticleDOI
Nanopore-Based Single-Entity Electrochemistry for the Label-Free Monitoring of Single-Molecule Glycoprotein-Boronate Affinity Interaction and Its Sensing Application.
TL;DR: Functionalized-nanopore-based single-entity electrochemistry analysis tools were developed for the label-free monitoring of single-molecule glycoprotein-boronate affinity interaction for the first time, and immunoglobulin G (IgG, one of the important biomarkers for many diseases such as COVID-19 and cancers), and the results indicate that this method has potential application value for the detection of glycoproteins in real samples.
Journal ArticleDOI
Biosensors, microfluidics systems and lateral flow assays for circulating microRNA detection: A review.
Niloofar Khandan-Nasab,Saeedeh Askarian,Arash Mohammadinejad,Seyed Hamid Aghaee-Bakhtiari,Taraneh Mohajeri,Reza Kazemi Oskuee +5 more
TL;DR: In this article, a review briefly discusses miRNAs detection' methods, with a particular focus on lateral flow assay, biosensors, and microfluidic systems as novel and practical procedures.
Journal ArticleDOI
A general controllable release amplification strategy of liposomes for single-particle collision electrochemical biosensing
TL;DR: In this paper , a single-particle collision electrochemical (SPCE) biosensor was constructed for H9N2 avian influenza virus detection by combining liposome encapsulation-release strategy with immunomagnetic separation.
References
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A DNA-fuelled molecular machine made of DNA
Bernard Yurke,Andrew J. Turberfield,Andrew J. Turberfield,Allen P. Mills,Friedrich C. Simmel,Jennifer L. Neumann +5 more
TL;DR: The construction of a DNA machine in which the DNA is used not only as a structural material, but also as ‘fuel’; each cycle produces a duplex DNA waste product.
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Triggered amplification by hybridization chain reaction.
Robert M. Dirks,Niles A. Pierce +1 more
TL;DR: This work introduces the concept of hybridization chain reaction (HCR), in which stable DNA monomers assemble only upon exposure to a target DNA fragment, which allows DNA to act as an amplifying transducer for biosensing applications.
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Crystal structure of a laccase from the fungus Trametes versicolor at 1.90-A resolution containing a full complement of coppers.
TL;DR: This work presents the first crystal structure of an active laccase containing a full complement of coppers, the complete polypeptide chain together with seven carbohydrate moieties, and a mechanism is presented to explain how laccases could tune their redox potential by as much as 200 mV.
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Quinone chemistry and toxicity
Journal ArticleDOI
Observing single nanoparticle collisions at an ultramicroelectrode by electrocatalytic amplification.
Xiaoyin Xiao,Allen J. Bard +1 more
TL;DR: Applications to studying heterogeneous kinetics at single nanoparticles, determining particle size distributions, and as a very sensitive electroanalytical technique are suggested.