Strain-specific probiotics properties of Lactobacillus fermentum, Lactobacillus plantarum and Lactobacillus brevis isolates from Brazilian food products
TL;DR: The present work showed that the probiotic characteristics were strain-specific and that the isolates L. plantarum CH3 and CH41 and L.brevis FFC199 exhibited potential probiotics properties.
About: This article is published in Food Microbiology.The article was published on 2013-10-01 and is currently open access. It has received 247 citations till now. The article focuses on the topics: Lactobacillus fermentum & Lactobacillus plantarum.
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01 Jan 2004
TL;DR: In this paper, a probiotic strain L. acidophilus M92 was examined for autoaggregation ability and cell surface hydrophobicity, which suggests the possible role of cell surface layer (S-layer) proteins, approximated at 45 kDa.
Abstract: The ability of probiotic bacteria to adhere to the intestinal epithelium play an important role in colonization of the gastrointestinal tract, preventing their elimination by peristalsis and providing a competitive advantage in this ecosystem. To identify bacterial traits related to adhesion the probiotic strain L. acidophilus M92 was examined for autoaggregation ability and cell surface hydrophobicity L. acidophilus M92 exhibits a strong autoaggregation phenotype and also coaggregation with some pathogen microorganisms that may form a barrier that prevents their colonization. The examined probiotic strain manifests a good degree of hydrophobicity determined by microbial adhesion to hydrocarbons. Aggregation and hydrophobicity were abolished upon exposure of the cells to pronase, which suggests the possible role of cell surface layer (S-layer) proteins, approximated at 45 kDa, in a L. acidophilus M92. The relationship between autoaggregation and adhesion ability to intestinal tissue was investigated by observing the adhesivity of L. acidophilus M92 to porcine ileal epithelial cells. Removal of the S-layer proteins by extraction with 5 M LiCl reduced autaggregation and in vitro adhesion of this strain.
605 citations
TL;DR: The recent developments in the processes, strategies, and methods, such as anticarcinogenic, antidepression, antianxiety, antiobesity, antidiabetic, immunostimulatory, and cholesterol-lowering assessments, are reviewed to select probiotic strains with the ultimate objective of assisting future probiotic microbe evaluation studies.
257 citations
TL;DR: The results suggest that these potential probiotic strains can be used as alternatives for control of biofilm formation by pathogenic bacteria in the food industry, without conferring a risk to the consumers.
Abstract: Use of probiotic biofilms can be an alternative approach for reducing the formation of pathogenic biofilms in food industries. The aims of this study were (i) to evaluate the probiotic properties of bacteriocinogenic (Lactococcus lactis VB69, L. lactis VB94, Lactobacillus sakei MBSa1, and Lactobacillus curvatus MBSa3) and non-bacteriocinogenic (L. lactis 368, Lactobacillus helveticus 354, Lactobacillus casei 40, and Weissela viridescens 113) lactic acid bacteria (LAB) isolated from Brazilian's foods and (ii) to develop protective biofilms with these strains and test them for exclusion of Listeria monocytogenes, Escherichia coli O157:H7, and Salmonella Typhimurium. LAB were tested for survival in acid and bile salt conditions, surface properties, biosurfactant production, β-galactosidase and gelatinase activity, antibiotic resistance and presence of virulence genes. Most strains survived exposure to pH 2 and 4% bile salts. The highest percentages of auto-aggregation were obtained after 24 h of incubation. Sixty-seven percentage auto-aggregation value was observed in W. viridescens 113 and Lactobacillus curvatus MBSa3 exhibited the highest co-aggregation (69% with Listeria monocytogenes and 74.6% with E. coli O157:H7), while the lowest co-aggregation was exhibited by W. viridescens 113 (53.4% with Listeria monocytogenes and 38% with E. coli O157:H7). Tests for hemolytic activity, bacterial cell adherence with xylene, and drop collapse confirmed the biosurfactant-producing ability of most strains. Only one strain (L. lactis 368) produced β-galactosidase. All strains were negative for virulence genes cob, ccf, cylLL, cylLs, cyllM, cylB, cylA and efaAfs and gelatinase production. The antibiotic susceptibility tests indicated that the MIC for ciprofloxacin, clindamycin, gentamicin, kanamycin, and streptomycin did not exceed the epidemiological cut-off suggested by the European Food Safety Authority. Some strains were resistant to one or more antibiotics and resistance to antibiotics was species and strain dependent. In the protective biofilm assays, strains L. lactis 368 (bac-), Lactobacillus curvatus MBSa3 (bac+), and Lactobacillus sakei MBSa1 (bac+) resulted in more than six log reductions in the pathogens counts when compared to the controls. This effect could not be attributed to bacteriocin production. These results suggest that these potential probiotic strains can be used as alternatives for control of biofilm formation by pathogenic bacteria in the food industry, without conferring a risk to the consumers.
200 citations
Cites background from "Strain-specific probiotics properti..."
...Their functional requirements include tolerance to acid and bile, adherence to epithelial surfaces and antagonistic activity toward intestinal pathogens (Ramos et al., 2013; Peres et al., 2014)....
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TL;DR: The Lb.
161 citations
Cites result from "Strain-specific probiotics properti..."
...Similar results have been previously reported by other authors analyzing LAB strains from different environments (Delgado et al., 2007; Vinderola et al., 2008; Zago et al., 2011; Ramos et al., 2013)....
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TL;DR: A two-stage interpretative model of the time-dependence of the TEER of epithelial layers grown in a transwell during Escherichia coli challenges in the absence or presence of adhering bifidobacteria is developed, suggesting that single-time TEER is interpretable in two ways, depending whether increasing to or decreasing from its maximum.
Abstract: Mechanisms of gastrointestinal protection by probiotic bacteria against infection involve amongst others, modulation of intestinal epithelial barrier function. Trans-epithelial electrical resistance (TEER) is widely used to evaluate cellular barrier functions. Here, we developed a two-stage interpretative model of the time-dependence of the TEER of epithelial layers grown in a transwell during Escherichia coli challenges in the absence or presence of adhering bifidobacteria. E. coli adhesion in absence or presence of adhering bifidobacteria was enumerated using selective plating. After 4-8 h, E. coli challenges increased TEER to a maximum due to bacterial adhesion and increased expression of a tight-junction protein [zonula occludens-1 (ZO-1)], concurrent with a less dense layer structure, that is indicative of mild epithelial layer damage. Before the occurrence of a TEER-maximum, decreases in electrical conductance (i.e., the reciprocal TEER) did not relate with para-cellular dextran-permeability, but after occurrence of a TEER-maximum, dextran-permeability and conductance increased linearly, indicative of more severe epithelial layer damage. Within 24 h after the occurrence of a TEER maximum, TEER decreased to below the level of unchallenged epithelial layers demonstrating microscopically observable holes and apoptosis. Under probiotic protection by adhering bifidobacteria, TEER-maxima were delayed or decreased in magnitude due to later transition from mild to severe damage, but similar linear relations between conductance and dextran permeability were observed as in absence of adhering bifidobacteria. Based on the time-dependence of the TEER and the relation between conductance and dextran-permeability, it is proposed that bacterial adhesion to epithelial layers first causes mild damage, followed by more severe damage after the occurrence of a TEER-maximum. The mild damage caused by E. coli prior to the occurrence of TEER maxima was reversible upon antibiotic treatment, but the severe damage after occurrence of TEER maxima could not be reverted by antibiotic treatment. Thus, single-time TEER is interpretable in two ways, depending whether increasing to or decreasing from its maximum. Adhering bifidobacteria elongate the time-window available for antibiotic treatment to repair initial pathogen damage to intestinal epithelial layers.
152 citations
References
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TL;DR: A database containing 16S rRNA gene sequences of all prokaryotic type strains was generated and a web-based tool, named EzTaxon, was constructed to achieve identification of isolates based on pairwise nucleotide similarity values and phylogenetic inference methods.
Abstract: 16S rRNA gene sequences have been widely used for the identification of prokaryotes. However, the flood of sequences of non-type strains and the lack of a peer-reviewed database for 16S rRNA gene sequences of type strains have made routine identification of isolates difficult and labour-intensive. In the present study, we generated a database containing 16S rRNA gene sequences of all prokaryotic type strains. In addition, a web-based tool, named EzTaxon, for analysis of 16S rRNA gene sequences was constructed to achieve identification of isolates based on pairwise nucleotide similarity values and phylogenetic inference methods. The system developed provides users with a similarity-based search, multiple sequence alignment and various phylogenetic analyses. All of these functions together with the 16S rRNA gene sequence database of type strains can be successfully used for automated and reliable identification of prokaryotic isolates. The EzTaxon server is freely accessible over the Internet at http://www.eztaxon.org/
2,051 citations
"Strain-specific probiotics properti..." refers methods in this paper
...Searches in EzTaxon server (http://www. eztaxon.org/; Chun et al., 2007) were performed to determine the closest known relatives of the partial 16S rRNA gene sequences that were obtained....
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TL;DR: A number of health benefits have been claimed for probiotic bacteria such as Lactobacillus acidophilus, Bifidobacterium spp.
723 citations
"Strain-specific probiotics properti..." refers background in this paper
..., 2008) and several strains have been used for the development of functional and therapeutic foods (Shah, 2007)....
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...…of L. plantarum have previously been proven to be able to survive gastric transit and colonize the intestinal tract of humans and other mammals (Georgieva et al., 2008; Mathara et al., 2008) and several strains have been used for the development of functional and therapeutic foods (Shah, 2007)....
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TL;DR: To investigate aggregation and adhesion of Lactobacillus acidophilus M92 to porcine ileal epithelial cells in vitro, and the influence of cell surface proteins on autoaggregation and adhesiveness of this strain.
Abstract: B. K OS, J. SUSKOVIC ´ ,S. V U K O V I C´ ,M. SIMPRAGA, J. F RECE A ND S. M ATOSIC ´ . 2003. Aims: To investigate aggregation and adhesiveness of Lactobacillus acidophilus M92 to porcine ileal epithelial cells in vitro, and the influence of cell surface proteins on autoaggregation and adhesiveness of this strain. Methods and Results: Lactobacillus acidophilus M92 exhibits a strong autoaggregating phenotype and manifests a high degree of hydrophobicity determined by microbial adhesion to xylene. Aggregation and hydrophobicity were abolished upon exposure of the cells to pronase and pepsin. Sodium dodecyl sulphate polyacrylamide gel electrophoresis of cell surface proteins revealed the presence of potential surface layer (S-layer) proteins, approximated at 45 kDa, in L. acidophilus M92. The relationship between autoaggregation and adhesiveness to intestinal tissue was investigated by observing the adhesiveness of L. acidophilus M92 to porcine ileal epithelial cells. Removal of the S-layer proteins by extraction with 5 mol l )1 LiCl reduced autoaggregation and in vitro adhesion of this strain. Conclusions: These results demonstrate that there is relationship between autoaggregation and adhesiveness ability of L. acidophilus M92, mediated by proteinaceous components on the cell surface. Significance and Impact of the Study: This investigation has shown that L. acidophilus M92 has the ability to establish in the human gastrointestinal tract, which is an important determinant in the choice of probiotic strains.
686 citations
"Strain-specific probiotics properti..." refers background or methods in this paper
...Some authors have suggested that these surface properties correlate with their adhesive capacity (Kos et al., 2003; Kotzamanidis et al., 2010)....
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...Auto-aggregation and co-aggregation assays were performed according to Kos et al. (2003)....
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...Bacterial adhesion is initially based on nonspecific physicalechemical interactions between two surfaces and the physical and chemical characteristics of the bacterial cell surface depend on surface hydrophobicity (Kotzamanidis et al., 2010; Kos et al., 2003)....
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...The percentage of co-aggregation was calculated using the equation (Kos et al., 2003): Co aggregation ð%Þ ¼ðððAxþAyÞ=2Þ AðxþyÞ=ðAxþAyÞ=2Þ 100 where x and y represent each of the two isolates in the control tubes, and (x þ y) the mixture....
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...The percentage of co-aggregation was calculated using the equation (Kos et al., 2003):...
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Book•
01 Jan 1992
TL;DR: The immune state genetic manipulation of gut micro-organisms selection of strains for probiotic use and the indigenous gut flora gut flora and disease resistance factors affecting the microecology of the gut probiotics are studied.
Abstract: History and development of probiotics bacterial interactions in the gut metabolic interactions in the gut translocation and the indigenous gut flora gut flora and disease resistance factors affecting the microecology of the gut probiotics and the immune state genetic manipulation of gut micro-organisms selection of strains for probiotic use probiotics for chickens probiotics for pigs probiotics for ruminants probiotics for humans problems and prospects.
638 citations
"Strain-specific probiotics properti..." refers background in this paper
...According to Fuller (1992), bile, even at low concentrations, can inhibit the in vitro growth of microorganisms....
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01 Jan 2004
TL;DR: In this paper, a probiotic strain L. acidophilus M92 was examined for autoaggregation ability and cell surface hydrophobicity, which suggests the possible role of cell surface layer (S-layer) proteins, approximated at 45 kDa.
Abstract: The ability of probiotic bacteria to adhere to the intestinal epithelium play an important role in colonization of the gastrointestinal tract, preventing their elimination by peristalsis and providing a competitive advantage in this ecosystem. To identify bacterial traits related to adhesion the probiotic strain L. acidophilus M92 was examined for autoaggregation ability and cell surface hydrophobicity L. acidophilus M92 exhibits a strong autoaggregation phenotype and also coaggregation with some pathogen microorganisms that may form a barrier that prevents their colonization. The examined probiotic strain manifests a good degree of hydrophobicity determined by microbial adhesion to hydrocarbons. Aggregation and hydrophobicity were abolished upon exposure of the cells to pronase, which suggests the possible role of cell surface layer (S-layer) proteins, approximated at 45 kDa, in a L. acidophilus M92. The relationship between autoaggregation and adhesion ability to intestinal tissue was investigated by observing the adhesivity of L. acidophilus M92 to porcine ileal epithelial cells. Removal of the S-layer proteins by extraction with 5 M LiCl reduced autaggregation and in vitro adhesion of this strain.
605 citations