Structural organisations of hemoglobin and myoglobin influence their binding behaviour with phenothiazines
TL;DR: Binding modalities of chlorpromazine and trifluoperazine, two widely used antipsychotic phenothiazine drugs with hemoglobin and myoglobin have been studied to understand how the quaternary, tertiary and secondary structural organisations of the proteins regulate the binding process.
About: This article is published in International Journal of Biological Macromolecules.The article was published on 1998-07-01. It has received 40 citations till now. The article focuses on the topics: Myoglobin & Denaturation (biochemistry).
Citations
More filters
TL;DR: In this article, the adsorption of cytochrome c onto a range of different mesoporous silicates (MPS) was studied, with the pore diameter of the material, which was measured by N2 gas adaption, being crucial to mesopore penetration.
Abstract: The adsorption of cytochrome c onto a range of different mesoporous silicates (MPS) was studied. The materials used, templated using both cationic and nonionic surfactants, have average pore-size diameters in the range from 28 to 130 A. Cytochrome c was found to bind to all MPS investigated, with the pore diameter of the material, which was measured by N2 gas adsorption, being crucial to mesopore penetration. The adsorption of a range of proteins with isoelectric points between 1 and 10 was investigated. For adsorption to occur, the surface charges of the protein and of the MPS must be complementary, in addition to the requirement that the pore diameter be sufficiently large. Pepsin at pH 6.5, for example, is negatively charged and does not adsorb onto cyano-modified silicate whereas subtilisin, which is of a similar size and bears an overall positive charge, is adsorbed. Using resonance Raman spectroscopy, cytochrome c was observed to occur in both high spin and low spin states, in contrast to that in solution, where the protein is predominantly in the low spin state. The presence of the high spin state may account for the enhanced peroxidative activity of the adsorbed protein.
251 citations
TL;DR: Histopathology and several blood parameters indicated that oral administrations of nanoparticles were free from toxicity and suggested that polymeric formulations were quite effective for oral delivery of the flavonoid as a therapeutic agent in the treatment of dyslipidemia, hyperglycemia and haemoglobin iron-mediated oxidative stress in type 1 diabetic model.
144 citations
Cites methods from "Structural organisations of hemoglo..."
...Haemoglobin (Hb) was isolated and purified from red blood cells (RBC) by using Sephadex G-100 column chromatography (Bhattacharyya et al., 1998), and its concentration was measured from the Soret absorbance using extinction coefficient, ε415nm as 125 mM -1 cm-1 (monomer basis)....
[...]
TL;DR: It is demonstrated that in the presence of H(2)O(2), HbA(1c) degrades DNA and protein more efficiently than Hb a(0) and formation of carbonyl content, an index of oxidative stress, is higher by H bA( 1c).
111 citations
TL;DR: The study investigates the effect of glycyrrhizin on streptozotocin‐induced diabetic changes and associated oxidative stress, including haemoglobin‐induced free iron‐mediated oxidative reactions.
Abstract: Objectives Glycyrrhizin is the main water-soluble constituent of the root of liquorice (Glycyrrhiza glabra). The study investigates the effect of glycyrrhizin on streptozotocin (STZ)-induced diabetic changes and associated oxidative stress, including haemoglobin-induced free iron-mediated oxidative reactions.
Methods Male Wistar rats were grouped as normal control, STZ-induced diabetic control, normal treated with glycyrrhizin, diabetic treated with glycyrrhizin and diabetic treated with a standard anti-hyperglycaemic drug, glibenclamide. Different parameters were studied in blood and tissue samples of the rats.
Key findings Glycyrrhizin treatment improved significantly the diabetogenic effects of STZ, namely enhanced blood glucose level, glucose intolerant behaviour, decreased serum insulin level including pancreatic islet cell numbers, increased glycohaemoglobin level and enhanced levels of cholesterol and triglyceride. The treatment significantly reduced diabetes-induced abnormalities of pancreas and kidney tissues. Oxidative stress parameters, namely, serum superoxide dismutase, catalase, malondialdehyde and fructosamine in diabetic rats were reverted to respective normal values after glycyrrhizin administration. Free iron in haemoglobin, iron-mediated free radical reactions and carbonyl formation in haemoglobin were pronounced in diabetes, and were counteracted by glycyrrhizin. Effects of glycyrrhizin and glibenclamide treatments appeared comparable.
Conclusion Glycyrrhizin is quite effective against hyperglycaemia, hyperlipidaemia and associated oxidative stress, and may be a potential therapeutic agent for diabetes treatment.
101 citations
Cites methods from "Structural organisations of hemoglo..."
...Haemoglobin was isolated and purified from red blood cells (RBC) by using Sephadex G-100 column chromatography,([14]) and its concentration was measured from the Soret absorbance using extinction coefficient, e415nm as 125/mm/cm (monomer basis)....
[...]
TL;DR: Pelargonidin counteracts hemoglobin glycation, iron release from the heme protein and iron-mediated oxidative damages, confirming glycated hemoglobin-associated oxidative stress in diabetes.
95 citations
References
More filters
Book•
[...]
01 Jan 1998
TL;DR: This CD-ROM provides the tools to draw structures and then search for them, and presents over 10,000 monographs which detail chemicals, drugs and biologicals, and describe a single substance or small group of related compounds.
Abstract: Presents over 10,000 monographs which detail chemicals, drugs and biologicals, and describe a single substance or small group of related compounds This CD-ROM provides the tools to draw structures and then search for them Users are able to access the data they need quickly with a choice of search options: text search, structure search, and sub-structure search Text searching is possible for every field of the database: chemical, common, generic and brand names; molecular weights and formulae; physical and toxicity data; and citations to the scientific and patent literature New with this release are organic name reactions available as a fully text searchable file, and 100 new monographs detailing the latest new drugs and compounds of recent interest This CD-ROM should be of interest to medicinal chemists, pharmaceutical chemists, biochemists, environmentalists, medical practitioners, natural product chemists, biologists, food scientists, pharmacologists, clinical chemists, organic chemists, inorganic chemists, and information professionals
3,577 citations
1,853 citations
Journal Article•
TL;DR: The fourth edition of this very successful textbook was reviewed in NATUBE of January 6, 1934, p. 8, so there can be no questioning its utility, especially to medical students, to whom it is primarily addressed.
Abstract: THE fourth edition of this very successful textbook was reviewed in NATUBE of January 6, 1934, p. 8. There have been a total of ten editions and reprintings since the original publication of the book ten years ago, so there can be no questioning its utility, especially to medical students, to whom it is primarily addressed.A Text-Book of Biochemistry: for Students of Medicine and Science. By Prof. A. T. Cameron. (Churchill's Empire Series.) Fifth edition. Pp. viii + 414. (London: J. and A. Churchill, Ltd., 1938.) 15s.
155 citations
TL;DR: The ability of the various salts to promote dissociation did not depend only on their contribution to the ionic strength of the solvent but also, and in some cases in large part, depended on specific interactions between the salt ions and the protein molecules.
118 citations
TL;DR: Binding of chlorpromazine with human hemoglobin has been studied by equilibrium dialysis and fluorescence quenching and results revealed that the binding was positively cooperative with overall affinity constant K = 3.8 x 10(3) M-1.
102 citations