Structure and Dynamics of Thermosensitive pDNA Polyplexes Studied by Time-Resolved Fluorescence Spectroscopy.
Lies A. L. Fliervoet,Ekaterina S. Lisitsyna,Nikita A. Durandin,Ilias Kotsis,Roel F. Maas-Bakker,Marjo Yliperttula,Wim E. Hennink,Elina Vuorimaa-Laukkanen,Tina Vermonden +8 more
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TLDR
The structure and dynamics of thermosensitive polyplexes were investigated by tracking the behavior of labeled plasmid DNA and polymer with time-resolved fluorescence spectroscopy using fluorescence resonance energy transfer (FRET) and showed to have a dynamic character meaning that the polymer chains can exchange between the polyplex core and shell.About:
This article is published in Biomacromolecules.The article was published on 2020-01-13 and is currently open access. It has received 5 citations till now. The article focuses on the topics: Poly(N-isopropylacrylamide) & Atom-transfer radical-polymerization.read more
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Principles Of Fluorescence Spectroscopy
TL;DR: The principles of fluorescence spectroscopy is universally compatible with any devices to read and is available in the digital library an online access to it is set as public so you can download it instantly.
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Progress of cationic gene delivery reagents for non-viral vector
TL;DR: In this article, the authors summarized the approaches and developments of types and components of transfection reagents and optimization of gene delivery and discussed the optimization of mammalian cell transient recombinant protein expression system and cationic reagents for clinical or clinical trials.
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Future of Biomacromolecules at a Crossroads of Polymer Science and Biology
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Self-Healing Thermosensitive Hydrogel for Sustained Release of Dexamethasone for Ocular Therapy
TL;DR: In this article , a self-healing hydrogel consisting of a thermosensitive ABA triblock copolymer was designed for sustained ocular delivery of dexamethasone.
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Histidine-Based Reduction-Sensitive Star-Polymer Inclusion Complex as a Potential DNA Carrier: Biophysical Studies Using Time-Resolved Fluorescence as an Important Tool.
TL;DR: Here, it is successfully demonstrated the synthesis of a novel biocompatible star-shaped inclusion complex with potential to carry and release DNA in cancer cell and the feasibility of using time-resolved fluorescence spectroscopic technique to study the complexation behavior of polycation and DNA using TO as a DNA intercalating dye.
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Book
Principles of fluorescence spectroscopy
TL;DR: This book describes the fundamental aspects of fluorescence, the biochemical applications of this methodology, and the instrumentation used in fluorescence spectroscopy.
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Tissue sulfhydryl groups
TL;DR: A water-soluble (at pH 8) aromatic disulfide [5,5′-dithiobis(2-nitrobenzoic acid] has been synthesized and shown to be useful for determination of sulfhydryl groups.
Journal ArticleDOI
Emerging applications of stimuli-responsive polymer materials
Martien A. Cohen Stuart,Wilhelm T. S. Huck,Jan Genzer,Marcus Müller,Christopher K. Ober,Manfred Stamm,Gleb B. Sukhorukov,Igal Szleifer,Vladimir V. Tsukruk,Marek W. Urban,Françoise M. Winnik,Stefan Zauscher,Igor Luzinov,Sergiy Minko +13 more
TL;DR: This work reviews recent advances and challenges in the developments towards applications of stimuli-responsive polymeric materials that are self-assembled from nanostructured building blocks and provides a critical outline of emerging developments.
Principles Of Fluorescence Spectroscopy
TL;DR: The principles of fluorescence spectroscopy is universally compatible with any devices to read and is available in the digital library an online access to it is set as public so you can download it instantly.
Journal ArticleDOI
Determination of free amino groups in proteins by trinitrobenzenesulfonic acid
TL;DR: A sensitive method to determine the free amino groups in proteins is presented that makes use of the reagent 2,4,6-trinitrobenzenesulfonic acid and found that sodium dodecyl sulfate was bound to some of the ϵ-amino groups of lysine in bovine serum albumin, ovalbumin, and human γ-globulin and rendered the involved amino groups unreactive toward TNBS.