Structure of purple membrane from halobacterium halobium: recording, measurement and evaluation of electron micrographs at 3.5 Å resolution
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...The defocus parameters can also be determined by a least-squares fit between the calculated CTF and square root of the observed power spectrum (Frank, 1972) or between the square of the calculated CTF and the power spectrum (Henderson et al., 1986)....
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...The oscillations visible in the power spectrum of an electron micrograph (Thon rings; Thon, 1966) have been used before to determine defocus parameters for untilted specimens (for example, Conway and Steven, 1999; Frank, 1972; Henderson et al., 1986; Ludtke et al., 1999; Morgan et al., 1995; Tani et al., 1996; Zhu et al., 1997), and to obtain tilt information (Henderson and Unwin, 1975; van Heel et al....
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