Studies on amino acid utilization in relation to virulence of Macrophomina phaseoli causing disease of jute (corchorus capsularis L.)
30 Apr 1974-Mycopathologia Et Mycologia Applicata (Mycopathol Mycol Appl)-Vol. 52, Iss: 3, pp 273-281
TL;DR: Efficiency of amino acid utilization could be regarded as one of the important biochemical characteristics of the virulent species.
Abstract: Amino acid utilization and synthetic capacity of two strains (JR & IR) ofMacrophomina phaseoli, a jute parasite, were compared in relation to their virulence. The effects of an extraneous supply of appropriate amino acid on the pathogenicity of the avirulent strain (IR) were also studied. Chromatographic and growth studies revealed that the avirulent strain contained a greater number of amino acids but its utilizing capacity was much lower than that of the virulent strain (JR). Out of 16 amino acids and an amide tested, phenylalanine was found to be most suitable for the growth of the IR strain, the optimum concentration being 0.5 per cent. This concentration did not, however, affect the resistance of the host after foliar application but temporarily increased the pathogenicity when it was given to the pathogen (IR) in vitro. Phenylalanine was detected neither in mycelia of the tested strains nor in the healthy roots of host plants under test conditions. Efficiency of amino acid utilization could be regarded as one of the important biochemical characteristics of the virulent species.
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TL;DR: A red pigment found in muskmelons infected by Macrophomina phaseolina was produced in liquid cultures of the pigment-producing isolate, TX-33, supplied with glycine, and feeding studies with purine bases presented no evidence for a relationship between purine biosynthesis and pigment production.
Abstract: A red pigment found in muskmelons (Cucumis melo) infected by Macrophomina phaseolina was produced in liquid cultures of the pigment-producing isolate, TX-33, supplied with glycine. In addition to glycine, alanine, leucine and asparagine supported synthesis of the red pigment when added to cultures of TX-33. A number of other amino acids and nitrogencontaining compounds failed to elicit pigment synthesis. Growth was stimulated over 5-fold when alanine, histidine or asparagine was substituted for glycine in the culture medium. However, growth was not strongly correlated with synthesis of pigments. Feeding studies with purine bases presented no evidence for a relationship between purine biosynthesis and pigment production.
4 citations
TL;DR: Single hyphal tip cultures of Macrophomina phaseolina, isolated from the lesions on pegs, pods, roots and stems of naturally infected groundnut plant, were analyzed for amino acid composition of the mycelium.
Abstract: Single hyphal tip cultures of Macrophomina phaseolina, isolated from the lesions on pegs, pods, roots and stems of naturally infected groundnut plant, were analyzed for amino acid composition of the mycelium. The isolates varied, both qualitatively and also quantitatively in the composition of amino acids, both free and bound forms.
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TL;DR: The pattern of susceptibility and resistance of pea varieties inoculated with biochemical mutants of F. oxysporum f.
Abstract: 1. One isolate of race 1 of Fusarium oxysporum Fr. f. pisi (Linf.) Snyder and Hansen was virulent for five varieties of pea (Pisum sativum L.), and a second isolate of this race was virulent for only two of these varieties. The two isolates of race 2 of this fungus were virulent for two of the five varieties. 2. Five pea varieties displayed a pattern of susceptibility and resistance to biochemical mutants of race 1; two of these pea varieties also displayed a similar pattern to biochemical mutants of race 2. 3. Sections of root and stem from susceptible varieties added to a minimal medium provided the nutritional requirements of all virulent mutants; all mutants that did not grow when sections of root and stem were added were avirulent or, at most, of questionable virulence for the varieties that supplied the sections. 4. The fungus was isolated from nineteen of twenty plants that had been inoculated with mutants, and, in each case, the fungus was still mutant. 5. The pattern of susceptibility and resista...
31 citations
TL;DR: Some host–parasite interactions of the C. fulvum – tomato leaf complex have been correlated with degrees of utilization in vitro by races of the pathogen of metabolites which have been shown to occur in different amounts in some tomato hosts which react differentially to them.
Abstract: Some host–parasite interactions of the C. fulvum – tomato leaf complex have been correlated with degrees of utilization in vitro by races of the pathogen of metabolites which have been shown to occur in different amounts in some tomato hosts which react differentially to them. Similarities and differences in the behavior of three pathogenic races were noted when the plants were grown in a number of amino nitrogen and sugar carbon sources. Similarities are interpreted as representing species characteristics; on the other hand, differences, including colony colors in glutamine and γ-aminobutyric acid, appear to be racial characteristics. Further evidence suggesting that certain host metabolites modify or condition the pathogenic expression was obtained from studies of the effect on pathogenicity of culture media differing in amino nitrogen content. From a comparison of the metabolites of host–pathogen complexes of differing reaction types, some insight was gained into probable nutritional requirements for a...
13 citations