Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
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TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
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Direct conversion of cellulosic material to ethanol by the intergeneric fusant Trichoderma reesei QM 9414/Saccharomyces cerevisiae NCIM 3288
TL;DR: Single-stage direct bioconversion of cellulosic materials to ethanol, under almost the same fermentation conditions applied to the Saccharomyces cerevisiae-ethanol process, was attempted using intergeneric fusants of Trichoderma reesei QM 9414/S.
Journal ArticleDOI
Intergeneric hybridization of Trichoderma reesei QM9414 and Saccharomyces cerevisiae NCIM 3288 by protoplast fusion
J. Anjani Kumari,Tapobrata Panda +1 more
TL;DR: An attempt to produce ethanol from a cellulosic substrate by a single-stage process using intergeneric hybrids obtained from Trichoderma reesei QM 9414 and Saccharomyces cerevisiae NCIM 3288 fusants is described, showing the highest synthesis of ethanol from filter paper cellulose.
Journal ArticleDOI
Production and regeneration of protoplasts from orchid Mycorrhizal Fungi Epulorhiza repens and Ceratorhiza sp.
Irene da Silva Coelho,Marisa Vieira de Queiroz,Maurício Dutra Costa,Maria Catarina Megumi Kasuya,Elza Fernandes de Araújo +4 more
TL;DR: The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp.
Journal ArticleDOI
Control of Stemphylium Leaf Blight Disease of Onion and Elevation of Seed Production Using Certain Bioagents
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Growth and pectinase production by Aspergillus Mexican strain protoplast regenerated under acidic stress.
TL;DR: Regeneratio at extreme pH improved the performance of LpH-10 strain, derived from protoplast from Aspergillus sp FP-180, which was able to regenerate at pH 1.7 and grow at pH values as low as 1.5, values at which the original strain is unable to grow.
References
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A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
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Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Fungal protoplasts: isolation, reversion, and fusion
TL;DR: The protoplast Fusion and the Hybridization of Fungal Species and Transformation in Fungi by Using Protoplasts by using Enzymatic and Nonenzymatic methods are studied.
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Induced fusion of fungal protoplasts following treatment with polyethylene glycol.
Jozef Anné,John F. Peberdy +1 more
TL;DR: Heterokaryon formation, at high frequency, after induced intraspecific fusion between protoplasts of auxotrophs of Penicillium chryosogiwim, P .
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A comparison of methods for estimating the viable count of a suspension of tumour cells.
TL;DR: All four in vitro methods of estimating the percentage viability of a cell suspension gave similar results with a recently harvested cell suspension but three of them grossly overestimated the proportion of viable cells in a suspension which had been stored at 4 °C for 11 days.