Journal ArticleDOI
Studies on critical analysis of factors influencing improved production of protoplasts from Trichoderma reesei mycelium
J. Anjani Kumari,Tapobrata Panda +1 more
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TLDR
The most suitable conditions for protoplasting were as follows: age of the organism in slant, 3 days; mycelium age, 20 h; volume of lytic enzymes, 190 ml;Mycelial weight (dry equivalent), 1.66 g; time of contact with lytic enzyme, 2 h; temperature of protoplasts, 30°C; phosphate buffer, 25 m m , pH 6.5; KCl as osmotic stabilizer, 0.7 mAbout:
This article is published in Enzyme and Microbial Technology.The article was published on 1992-03-01. It has received 43 citations till now. The article focuses on the topics: Trichoderma longibrachiatum & Mycelium.read more
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Dissertation
Characterization of double-stranded RNA (dsRNA) from Rhizoctonia solani
TL;DR: Overall, the study indicated the ubiquity of dsRNA in R. solani strains, but no firm correlation could be found between the presence ofdsRNA and the degree of virulence of strains, and vegetative incompatibility seen in this study would indicate the difficulty of achieving transmission of any hypovirulent ds RNA in field populations of R.solani.
Journal ArticleDOI
Analysis of factors responsible for the regeneration to intact cells from sphaeroplasts of Saccharomyces cerevisiae
J. Anjani Kumari,Tapobrata Panda +1 more
TL;DR: The regeneration of Saccharomyces cerevisiae cells from its sphaeroplasts were found to be influenced by a number of factors, including malt-extractglucose-yeast extract-peptone medium and growth medium.
Book ChapterDOI
Protoplast fusion in disease control
Sumeet,K. G. Mukerji +1 more
TL;DR: The use of ecologically safe and environmentally friendly methods of protecting crops from pests and pathogens is gaining importance and beneficial microbes are rarely used to control plant diseases primarly.
Journal ArticleDOI
Improved technique on reversion of mycelial protoplast of Trichoderma reesei into mycelia
J. Anjani Kumari,Tapobrata Panda +1 more
TL;DR: Factors influencing the regeneration of protoplasts of T. longibrachiatum QM 9414 were found to be, the nature of osmotic stabilizer, the concentration of o smotic stabilizers, pH, temperature, and the composition of regeneration medium.
Journal ArticleDOI
Ursodeoxycholic acid production by Gibberella zeae mutants
V.V. Kollerov,Marina V. Donova +1 more
TL;DR: In this paper , an ascomycete Gibberella zeae VKM F-2600 is capable of producing UDCA by 7β-hydroxylation of lithocholic acid (LCA).
References
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Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Fungal protoplasts: isolation, reversion, and fusion
TL;DR: The protoplast Fusion and the Hybridization of Fungal Species and Transformation in Fungi by Using Protoplasts by using Enzymatic and Nonenzymatic methods are studied.
Journal ArticleDOI
Induced fusion of fungal protoplasts following treatment with polyethylene glycol.
Jozef Anné,John F. Peberdy +1 more
TL;DR: Heterokaryon formation, at high frequency, after induced intraspecific fusion between protoplasts of auxotrophs of Penicillium chryosogiwim, P .
Journal ArticleDOI
A comparison of methods for estimating the viable count of a suspension of tumour cells.
TL;DR: All four in vitro methods of estimating the percentage viability of a cell suspension gave similar results with a recently harvested cell suspension but three of them grossly overestimated the proportion of viable cells in a suspension which had been stored at 4 °C for 11 days.