Journal ArticleDOI
Studies on improved techniques for immobilizing and stabilizing penicillin amidase associated with E. coli cells
P.S.R. Babu,Tapobrata Panda +1 more
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TLDR
The whole cell immobilization technique has been optimized for different process parameters and the granular catalyst has good mechanical strength, low protein leachability, and high retention of penicillin amidase activity.About:
This article is published in Enzyme and Microbial Technology.The article was published on 1991-08-01. It has received 24 citations till now. The article focuses on the topics: Penicillin amidase activity & Penicillin amidase.read more
Citations
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Journal ArticleDOI
Development of novel conductometric biosensors based on immobilised whole cell Chlorella vulgaris microalgae.
TL;DR: It finally appeared that conductometric biosensors using algae seemed more sensitive than bioassays to detect low levels of cadmium ions (the detection limit for the first experiments was 1 ppb of Cd2+).
Journal ArticleDOI
Microencapsulated bacterial cells can be used to produce the enzyme feruloyl esterase: preparation and in-vitro analysis
Jasmine Bhathena,Arun Kulamarva,Aleksandra Malgorzata Urbanska,Christopher Martoni,Satya Prakash +4 more
TL;DR: Results show that ferulic acid can be produced using microencapsulated Lactobacillus fermentum with significant levels of biological feruloyl esterase activity.
Journal ArticleDOI
Chlorella sp. based biosensor for selective determination of mercury in presence of silver ions
TL;DR: In this article, a whole cell based biosensor is prepared by immobilizing Chlorella sp. microbes over glassy carbon electrode, which is optimized for characteristics like substrate concentration, pH, response time, and durability.
Journal ArticleDOI
Toward a new generation of therapeutics: artificial cell targeted delivery of live cells for therapy.
TL;DR: The current clinical status of probiotics is summarized, the promises and challenges of current immobilization technologies are examined, the concept of artificial cells for effective delivery of therapeutic bacterial cells are presented, and microencapsulation in “artificial cells” is presented.
Journal ArticleDOI
Whole cell environmental biosensor on diamond
TL;DR: It is found that the unique surface properties of diamond reduce the electrode fouling problem commonly encountered with metal electrodes.
References
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Journal ArticleDOI
Covalent coupling of microorganisms to a cellulosic support
TL;DR: This general technique for whole‐cell immobilization offers an advantage over entrapment methods in that the cells are attached to the outer surface of the solid, thus eliminating the resistance of a gel to the transfer of nutrients and products.
Book ChapterDOI
Immobilization of Whole Microbial Cells for the Production of 6- Amino Penicillanic Acid
TL;DR: In the process of developing a most efficient immobilized cell system for the production of 6 APA, two routes have been followed: increase of the specific activity of the cell system itself and selection of the appropriate immobilization technique.
Journal ArticleDOI
Production of 6-aminopenicillanic acid by immobilized Pleurotus ostreatus
TL;DR: 6-Aminopenicillanic acid from penicillin V is produced by Pleurotus ostreatus immobilized by entrapment in a chitosan matrix in carriers in which the cell concentration increases after network formation by irreversible shrinking of the biocatalyst.
Journal ArticleDOI
Penicillin acylase from the hybrid strains Escherichia coli 5K(pHM12): enzyme formation and hydrolysis of beta-lactam antibiotics with whole cells.
U. Schömer,A. Segner,F. Wagner +2 more
TL;DR: It is found that high-pressure liquid chromatography is to be preferred for the estimation of beta-lactamase activity during batch fermentation of E. coli 5K(pHM12), problems of plasmid stability in the host strain arose which were overcome by the addition of 4 mg of tetracycline per liter to the medium as a selective marker.
Journal ArticleDOI
The role of phenylacetic acid in biosynthesis of penicillin amidase in E. coli
P. S. R. Babu,Tapobrata Panda +1 more
TL;DR: For improved biosynthesis of penicillin amidase by E. coli, NCIM 2400, the participation of different carboxylic acids and polyols has been studied in association with the effect of phenylacetic acid in a modified defined medium.
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Optical algal biosensor using alkaline phosphatase for determination of heavy metals.
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