Journal ArticleDOI
Studies on intestinal protease: Isolation, purification and effect of dietary proteins on alkaline protease activity of the air-breathing fish, Clarias batrachus (Linn.)
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TLDR
In a study on the determination of sub-cellular localisation, it has been found that protease activity is more prominent in lysosomes than in other organelles of the cell.Abstract:
1.
The effect of dietary protein levels on the proteolytic activity in the intestines of the air-breathing fish, Clarias batrachus (Linn.) has been studied
2.
Activity of proteolytic enzymes increased significantly in fishes maintained with a 50% protein diet from those maintained with a 25% protein diet; still higher dietary protein percentage showed no further stimulation of enzyme activity.
3.
In a study on the determination of sub-cellular localisation, it has been found that protease activity is more prominent in lysosomes than in other organelles of the cell.
4.
A sixty fold purification of alkaline protease from the intestine of Clarias batrachus has been achieved by ion exchange chromatography on DEAE cellulose which has been further checked by polyacrylamide gel electrophoresis.read more
Citations
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Journal ArticleDOI
Effect of dietary protein on growth, feed conversion, body composition and survival of pike perch fingerlings (Sander lucioperca)
TL;DR: Dry matter and crude lipid content of pike perch fingerlings decreased with increasing dietary protein supply and significantly the lowest dry matterand crude lipid levels were observed in fish fed diets containing 619 g kg−1 of crude protein.
Journal ArticleDOI
Water quality, biofilm production and growth of fringe-lipped carp (Labeo fimbriatus) in tanks provided with two solid substrates
TL;DR: The results demonstrated that production of L. fimbriatus can be significantly increased by the introduction of biodegradable substrates into culture systems where fertilization and feeding are employed.
Journal ArticleDOI
Biochemical changes in the air-breathing catfish Clarias batrachus (linn.) exposed to malathion
TL;DR: Children of Clarias batrachus exposed for 40 days to malathion caused little alteration in its hepatic protein and nucleic acids but a marked decrease in liver glycogen and a significant increase in serum glucose and free amino acid level.
Journal ArticleDOI
Effects of dietary protein levels on the growth, energy budget, and physiological and immunological performance of green, white and purple color morphs of sea cucumber, Apostichopus japonicus
Yucen Bai,Libin Zhang,Sudong Xia,Shilin Liu,Xiaoshang Ru,Qinzeng Xu,Tao Zhang,Hongsheng Yang +7 more
TL;DR: Results showed that dietary protein levels had a significant effect on the growth performance of A. japonicus, indicating that the protein content was beyond the digestive ability of sea cucumber, and that excess protein in the diet inhibits protein digestion.
Journal ArticleDOI
Digestive enzyme complement of white sturgeon (Acipenser transmontanus).
TL;DR: Digestive processes of 250-day-old white sturgeon were analyzed for enzyme concentrations, protein content and pH and domination of the enzyme complement by proteases is consistent with this species carnivorous feeding habits.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
A modified spectrophotometric determination of chymotrypsin, trypsin, and thrombin.
TL;DR: The spectrophotometric procedure proposed by Schwert and Takenaka has been modified and extended to include the application to N-benzoyl-L-tyrosine ethyl ester and α-p-toluenesulphonyl- L-arginine methyl ester, allowing the determination of traces of chymotrypsin in the presence of relatively large amounts of trypsin.
Book ChapterDOI
[1] Column chromatography of proteins: Substituted celluloses
TL;DR: Chromatography of proteins on cellulose ion exchangers involves primarily the establishment of multiple electrostatic bonds between charged sites on the surface of the adsorbent and sites bearing the opposite charge on thesurface of the protein molecule.