Journal ArticleDOI
Studies on migration inhibitory factor (MIF): Recovery of MIF activity after purification by gel filtration and disc electrophoresis☆
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MIF activity could be separated from albumin on the basis of size and charge as demonstrated by Sephadex G-100 gel filtration, sucrose gradient centrifugation, and disc electrophoresis.About:
This article is published in Cellular Immunology.The article was published on 1970-05-01. It has received 122 citations till now. The article focuses on the topics: Sephadex.read more
Citations
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Journal ArticleDOI
Historical insights into cytokines
TL;DR: A recent advance in the field has been that of differential cytokine production, which can be used to classify human disease as being “ autoimmune” or “autoinflammatory” thus impacting on therapeutic interventions.
Journal ArticleDOI
Alterations of macrophage functions by mediators from lymphocytes
TL;DR: Macrophage adherence, phagocytosis, spreading, motility, and direct hexose monophosphate oxidation were enhanced, while protein synthesis was unaffected, so antigen-stimulated lymphocytes secrete a factor or factors which enhance certain macrophage functions.
Journal ArticleDOI
Mitogen requirements for the in vitro propagation of cutaneous T-cell lymphomas.
Adi F. Gazdar,Desmond N. Carney,Desmond N. Carney,Paul A. Bunn,Paul A. Bunn,Edward K. Russell,Edward K. Russell,Elaine S. Jaffe,Elaine S. Jaffe,Geraldine P. Schechter,Geraldine P. Schechter,John G. Guccion,John G. Guccion +12 more
TL;DR: In this article, the authors attempted to generate continuous in vitro cultures from patients with mycosis fungoides and the Sezary syndrome (cutaneous T-cell lymphomas, CTCL).
Journal ArticleDOI
Activation of macrophages in vivo and in vitro. Correlation between hydrogen peroxide release and killing of Trypanosoma cruzi.
TL;DR: The ability of macrophages to release H2O2 in response to phorbol myristate acetate (PMA) could be induced in vivo and in vitro, and reversed in vivo, in a manner correlating closely with changes in trypanocidal activity.
Book ChapterDOI
In Vitro Approaches to the Mechanism of Cell-Mediated Immune Reactions
TL;DR: This chapter focusses on the study of cell-mediated immunity in vitro, principally in terms of explaining the effector process of the response, with general assumptions that all cell- mediated immune reactions have a common basis.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Disc electrophoresis – ii method and application to human serum proteins*
TL;DR: The technique of disc electrophoresis has been presented, including a discussion of the technical variables with special reference to the separation of protein fractions of normal human serum.
Journal ArticleDOI
A Method for Determining the Sedimentation Behavior of Enzymes: Application to Protein Mixtures
Robert G. Martin,Bruce N. Ames +1 more
TL;DR: Sucrose gradient centrifugation is found to be a suitable method for determining sedimentation coefficients of enzymes in protein mixtures and the sedimentation behavior of several of the enzymes in the pathway of histidine biosynthesis in S. typhimurium has been determined.
Journal ArticleDOI
Disc electrophoresis-i background and theory*
TL;DR: Some mechanisms that provide a rationale for the resolution afforded by zone electrophoresis in many gels will be detailed; the theory of some new modifications of zone electophoresis that have been designed to take maximum advantage of these mechanisms will be developed.
Journal ArticleDOI
Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity
Barry R. Bloom,Boyce Bennett +1 more
TL;DR: The cell type responsible for inhibition by antigen of migration in vitro of peritoneal exudate cells obtained from tuberculin-hypersensitive guinea pigs was studied and elaborated into the medium a soluble material capable of inhibiting migration of normal exudates.
Related Papers (5)
Mechanism of a Reaction in Vitro Associated with Delayed-Type Hypersensitivity
Barry R. Bloom,Boyce Bennett +1 more