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Journal ArticleDOI

Studies on pH and thermal deactivation of pectolytic enzymes from Aspergillus niger

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TLDR
The addition of protein doubled the half-life times of partially purified PMG, PG I and PG II and the interaction effect of pectolytic enzymes on deactivation was found to be significant.
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This article is published in Biochemical Engineering Journal.The article was published on 2003-10-01. It has received 108 citations till now.

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Journal ArticleDOI

The stability and thermodynamic parameters of a very thermostable and calcium-independent α-amylase from a newly isolated bacterium, Anoxybacillus beppuensis TSSC-1

TL;DR: The broad thermo- and pH-tolerance of this enzyme suggests potential commercial opportunities and the very compact structure of the enzyme and the transitional enzyme–substrate complex resisted denaturation at extreme temperatures and alkaline pH.
Journal ArticleDOI

Studies on productivity and characterization of polygalacturonase from Aspergillus giganteus submerged culture using citrus pectin and orange waste.

TL;DR: Investigation of some of the factors affecting polygalacturonase production by an Aspergillus giganteus strain found it necessary to characterize this pectinolytic activity, which showed good thermal stability and high stability in the pH range from pH 4.5 to 8.5.
Journal ArticleDOI

Enzyme stability, thermodynamics and secondary structures of α-amylase as probed by the CD spectroscopy.

TL;DR: The overall profile of the enzyme: high thermostability, alkalitolerance, calcium independent nature, dextrose equivalent values and resistance against chemical denaturants, solvents and surfactants suggest its commercial applications.
Journal ArticleDOI

Kinetic studies of laccase enzyme of Coriolus versicolor MTCC 138 in an inexpensive culture medium

TL;DR: This study forms the first report on the potential application of waste water cyanobacterial bloom and dyeing effluent as a medium for laccase production by C. versicolor MTCC138.
Journal ArticleDOI

Single-step purification and characterization of an extreme halophilic, ethanol tolerant and acidophilic xylanase from Aureobasidium pullulans NRRL Y-2311-1 with application potential in the food industry.

TL;DR: The xylanase from Aureobasidium pullulans NRRL Y-2311-1 produced on wheat bran was purified by a single-step chromatographic procedure and exhibits excellent characteristics and could, therefore, be a promising candidate for application in food and bio-industries.
References
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Journal Article

Protein Measurement with the Folin Phenol Reagent

TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI

The Activated Complex in Chemical Reactions

TL;DR: In this paper, the probability of the activated state is calculated using ordinary statistical mechanics, and the probability multiplied by the rate of decomposition gives the specific rate of reaction, and necessary conditions for general statistical treatment to reduce to the usual kinetic treatment are given.
Book ChapterDOI

[17] Amylases, α and β

Journal ArticleDOI

Applications of pectinases in the commercial sector: a review.

TL;DR: This review discusses various types of pectinases and their applications in the commercial sector.
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