Journal ArticleDOI
Studies on pH and thermal deactivation of pectolytic enzymes from Aspergillus niger
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TLDR
The addition of protein doubled the half-life times of partially purified PMG, PG I and PG II and the interaction effect of pectolytic enzymes on deactivation was found to be significant.About:
This article is published in Biochemical Engineering Journal.The article was published on 2003-10-01. It has received 108 citations till now.read more
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Strategies for improved production of phycobiliproteins (PBPs) by Oscillatoria sp. BTA170 and evaluation of its thermodynamic and kinetic stability
TL;DR: The pH and thermal tolerances of PBPs suggested that they may have potential for use as pigments in industry and the deactivation rate constant (kd) was found to be minimum in the temperature range from 10 to 40 °C and pH 7.5, and the maximum deactivation ofPBPs was found at pH 12 and 80C.
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Investigation of deactivation thermodynamics of lipase immobilized on polymeric carrier.
TL;DR: In this paper, the authors investigated biochemical thermo-kinetic stability of lipases immobilized on a biocompatible polymeric material and determined the thermal and solvent stability of polymer immobilized lipase.
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Kinetic and thermodynamic characterization of a novel Aspergillus aculeatus URM4953 polygalacturonase. Comparison of free and calcium alginate-immobilized enzyme
TL;DR: Polygalacturonase from Aspergillus aculeatus URM4953 was covalently immobilized on calcium alginate beads using glutaraldehyde as a cross-linking agent and showed satisfactory performance and features as well as great potential for future industrial applications.
Journal ArticleDOI
Purification, kinetic and thermodynamic studies of a new ribonuclease from a mutant of Aspergillus niger.
TL;DR: The RNase showed the strongest affinity with RNA as the substrate, and the highest catalytic efficiency for hydrolysis of the substrate at pH 3.5 and 65 degrees C, and Thermodynamic parameters for catalysis and thermal denaturation were determined.
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Performance improvement of Bacillus aryabhattai ITBHU02 for high‐throughput production of a tumor‐inhibitory L‐asparaginase using a kinetic model based approach
Yogendra Singh,S. K. Srivastava +1 more
TL;DR: Endogenous metabolism of the strain for L-asparaginase synthesis was thermostable up to 40°C, which makes the strain commercially important as it can be utilized for cost-effective L-asperaginase production within countries such as India, where 35–40°C temperatures are quite common.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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The Activated Complex in Chemical Reactions
TL;DR: In this paper, the probability of the activated state is calculated using ordinary statistical mechanics, and the probability multiplied by the rate of decomposition gives the specific rate of reaction, and necessary conditions for general statistical treatment to reduce to the usual kinetic treatment are given.
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Applications of pectinases in the commercial sector: a review.
TL;DR: This review discusses various types of pectinases and their applications in the commercial sector.