Studies on some enzymes and sialic acid during progestational contraceptive therapy.
TL;DR: NET-EN contraception did not alter the liver function enzymes, but there is a significant increase in AChE activity after two years, and Serum sialic acid level showed a transient increase up to one year, which however returned to control level later.
About: This article is published in Contraception.The article was published on 1984-06-01. It has received 1 citations till now. The article focuses on the topics: Liver function & Norethisterone enanthate.
TL;DR: Changes in the haematological indices seem to be due to stimulation of the erythropoietic system by NET-EN and may also be attributable to diminished blood loss due to amenorrhoea during the course of therapy.
TL;DR: A photometric method for determining acetylcholinesterase activity of tissue extracts, homogenates, cell suspensions, etc., has been described and Kinetic constants determined by this system for erythrocyte eholinesterases are presented.
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
TL;DR: In this paper, homogenates of guinea-pig polymorphonuclear leucocytes were separated by differential centrifugation into six particulate fractions and a soluble fraction.
Abstract: 1. Homogenates of guinea-pig polymorphonuclear leucocytes were separated by differential centrifugation into six particulate fractions and a soluble fraction. 2. The distributions in these fractions of protein, DNA, succinate dehydrogenase, beta-glucuronidase, peroxidase, alkaline phosphatase, acid phosphatase (against p-nitrophenyl phosphate and beta-glycerophosphate), cathepsin, and catalase were compared. 3. Almost all of the DNA sedimented in the first two pellets, indicating that the nuclei were relatively intact. 4. The four hydrolases and peroxidase showed different distribution patterns, although these activities were previously reported to be localized mainly in the single ;granule' fraction isolated from leucocytes. 5. The particles containing peroxidase, acid phosphatase and alkaline phosphatase all exhibited latency. Maximum activity for each enzyme was obtained at roughly similar concentrations of Triton X-100. 6. The acid phosphatase of these cells was distributed between two populations of particles that differed in both sedimentation characteristics and density. The acid phosphatase(s) of the two populations showed slightly different substrate specificities. This bimodal distribution was not an artifact of the procedure used to elicit the cells. 7. Catalase was recovered almost entirely in the soluble fraction and showed no latency in freshly prepared homogenates. No urate oxidase was detected. 8. We conclude that the ;granule' fraction of the polymorphonuclear leucocyte, as isolated by previous workers, contains at least three, probably more, populations of particles with different enzyme contents, and that these cells probably do not contain peroxisomes.
01 Jan 1970
TL;DR: It is concluded that the ;granule' fraction of the polymorphonuclear leucocyte, as isolated by previous workers, contains at least three, probably more, populations of particles with different enzyme contents, and that these cells probably do not contain peroxisomes.
Abstract: cathepsin, andcatalase were compared. 3.AlmostalloftheDNA sedimented inthefirst twopellets, indicating thatthenuclei were relatively intact. 4.Thefourhydrolases andperoxidase showed different distribution patterns, although theseactivities were previously reported tobelocalized mainly inthesingle 'granule' fraction isolated fromleucocytes. 5.The particles containing peroxidase, acidphosphatase andalkaline phosphatase all exhibited latency. Maximumactivity foreachenzyme was obtained atroughly similar concentrations ofTriton X-100.6.Theacidphosphatase ofthese cellswas distributed between twopopulations ofparticles thatdiffered inbothsedimentation characteristics anddensity. Theacidphosphatase(s) ofthetwopopulations showed slightly different substrate specificities. Thisbimodaldistribution was notan artifact oftheprocedure usedtoelicit thecells.7.Catalase was recovered almost entirely inthesoluble fraction andshowed no latency infreshly prepared homogenates.No urateoxidase was detected. 8.We conclude thatthe'granule' fraction ofthepolymorphonuclear leucocyte, as isolated byprevious workers, contains atleast three, probably more, populations ofparticles withdifferent enzyme contents, andthatthese cells probably donotcontain peroxisomes.