Studies on the anticlastogenic effect of turmeric and curcumin on cyclophosphamide and mitomycin C in vivo.
TL;DR: Although curcumin is reported to be the active chemopreventive principle in turmeric effective against a number of potential carcinogens in several experimental systems, it was virtually ineffective against the clastogenicity of CP or MMC at the doses tested.
About: This article is published in Food and Chemical Toxicology.The article was published on 1998-01-01. It has received 32 citations till now. The article focuses on the topics: Curcumin & Chromosome aberration.
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TL;DR: The results show that 50 μM curcumin in the presence of 100–200 μM copper induced DNA damage in murine lymphocytes, andCurcumin alone was capable of inducing DNA strand breaks under the tested conditions.
Abstract: Dietary polyphenolics, such as curcumin, have shown antioxidant and anti-inflammatory effects Some antioxidants cause DNA strand breaks in excess of transition metal ions, such as copper The aim of this study was to evaluate thein vitro effect of curcumin in the presence of increasing concentrations of copper to induce DNA damage in murine leukocytes by the comet assay Balb-C mouse lymphocytes were exposed to 50 μM curcumin and various concentrations of copper (10 μM, 100 μM and 200 μM) Cellular DNA damage was detected by means of the alkaline comet assay Our results show that 50 μM curcumin in the presence of 100–200 μM copper induced DNA damage in murine lymphocytes Curcumin did not inhibit the oxidative DNA damage caused by 50 μM H2O2 in mouse lymphocytes Moreover, 50 μM curcumin alone was capable of inducing DNA strand breaks under the tested conditions The increased DNA damage by 50 μM curcumin was observed in the presence of various concentrations of copper, as detected by the alkaline comet assay
46 citations
Cites background from "Studies on the anticlastogenic effe..."
...In vivo studies of chromosome aberrations (Vijayalaxmi 1980; Mukhopadhyay et al. 1998; Shukla et al. 2002), micronuclei (Vijayalaxmi 1980), or somatic mutation and recombination (el Hamss et al. 1999), report that curcumin is not genotoxic....
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...Although some in vivo studies report that curcumin is not genotoxic (Vijayalaxmi 1980; Mukhopadhyay et al. 1998; el Hamss et al. 1999; Shukla et al. 2002), we also found DNA damage in vitro reported by others (Antunes et al. 1999; Araujo et al. 1999, 2001; Blasiak et al. 1999a,b; Kelly et al. 2001)....
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...Although some in vivo studies report that curcumin is not genotoxic (Vijayalaxmi 1980; Mukhopadhyay et al. 1998; el Hamss et al. 1999; Shukla et al. 2002), we also found DNA damage in vitro reported by others (Antunes et al....
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...In vivo studies of chromosome aberrations (Vijayalaxmi 1980; Mukhopadhyay et al. 1998; Shukla et al. 2002), micronuclei (Vijayalaxmi 1980), or somatic mutation and recombination (el Hamss et al....
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TL;DR: It is found that HDFs are more sensitive to curcumin treatment than MCF7 cells, resulting in pronounced arrest of cell cycle progression and higher levels of cellular death, and its use as a dietary supplement or in cancer therapies has a double edge.
Abstract: Background: Curcumin is a yellow-orange pigment obtained from the plant Curcuma longa, which is known to exert beneficial effects in several diseases, including cancer. However, at high doses, it may produce toxic and carcinogenic effects in normal cells. In this context, we studied the effects of curcumin on normal human dermal fibroblast (HDF) cells and breast cancer cells (MCF7). Methods: We used cellular viability and growth assays to evaluate the antiproliferative action of curcumin, analyzed the endogenous glutathione levels, conducted cell cycle, apoptosis, and necrosis analyses, and performed immunodetection of glutathionylated and acetylated H3 histones. Results: We found that HDFs are more sensitive to curcumin treatment than MCF7 cells, resulting in pronounced arrest of cell cycle progression and higher levels of cellular death. In both cell types, the homeostasis of the redox cellular environment did not change after curcumin treatment; however, significant differences were observed in glutathione (GSH) levels and in S-glutathionylation of H3 histones. Conclusion: Curcumin administration can potentially confer benefits, but high doses may be toxic. Thus, its use as a dietary supplement or in cancer therapies has a double edge.
44 citations
Cites background from "Studies on the anticlastogenic effe..."
...In bone marrow cells of acutely treated mice [16] and in different tissues (e....
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TL;DR: The present results indicate that curcumin shows both genotoxicity and antigenotoxicity depending on its concentration, with a small but significant increase in the frequency of MN.
43 citations
TL;DR: Curcumin-induced chromosomal damage in CHO cells can be mediated by hydroxyl radical generation in the present experimental conditions and the clastogenic action of curcumin was statistically decreased in a dose-dependent manner in the presence of thiourea.
Abstract: Natural dietary antioxidants are extensively studied for their ability to protect cells from damage to DNA, protein, and lipids induced by antitumor agents or radiation that leads to the generation of free radical in normal cells in vivo and in vitro. Curcumin is a natural antioxidant known to possess therapeutic properties and has been reported to scavenge free radicals and to inhibit clastogenesis in mammalian cells. However, curcumin has been reported to induce a significant increase in the frequency of chromosomal aberrations in Chinese hamster ovary (CHO) cells. To investigate whether the clastogenic activity of curcumin in CHO cells in culture can be ascribed to a pro-oxidant behavior, mediated by free radical generation, experiments were carried out with the combination of curcumin (15 microg/ml) and thiourea (10, 20, or 40 microg/ml), a potent hydroxyl radical scavenger. The results showed that the clastogenic action of curcumin was statistically decreased in a dose-dependent manner in the presence of thiourea. These data have shown that curcumin-induced chromosomal damage in CHO cells can be mediated by hydroxyl radical generation in the present experimental conditions. Teratogenesis Carcinog. Mutagen. 21:175-180, 2001.
39 citations
TL;DR: The natural coloring curcumin, for example, showed antimutagenic potential in in vivo tests but was mutagenic in in vitro tests, emphasizing the importance of careful assessment and wide investigation into the possible Mutagenic and/or antimUTagenic activity of food colorings.
Abstract: Muitos compostos presentes nos alimentos, tanto naturalmente, como adicionados ou produzidos durante o processamento, ja foram testados quanto a mutagenicidade ou antimutagenicidade em diferentes sistemas experimentais. O grande numero de corantes para alimentos, naturais ou sinteticos, tem levado os pesquisadores a avaliar a mutagenicidade e/ou antimutagenicidade desses compostos. Alguns corantes sinteticos apresentaram potencial mutagenico e seu uso foi proibido em alguns paises. Muitos corantes naturais testados apresentaram potencial antimutagenico em pelo menos um sistema-teste, entretanto, isto nao quer dizer que os corantes naturais sao inocuos. O corante natural curcumina, por exemplo, apresentou potencial antimutagenico nos testes in vivo e foi mutagenico nos testes in vitro. Este paradoxo ressalta a importância de uma avaliacao criteriosa e ampla na avaliacao da possivel atividade mutagenica e/ou antimutagenica dos corantes.
38 citations
Cites background from "Studies on the anticlastogenic effe..."
...Resultados obtidos por Mukhopadhyay et al. (1998) em células da medula óssea de camundongos tratados com a cúrcuma e curcumina mostraram que estes corantes foram mutagênicos fracos....
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...…Natural Aditivo Aditivo Sistema Teste* 3; 5; 7; 8 9 2 9 13 2; 4; 5; 6 2 Tipo Sistema Teste* 3; 5 2 3 1 Referências Giri (1991) Ishidate (1984) Mukhopadhyay et al. (1998) Araújo et al. (1999) Moller et al. (1998) Giri et al. (1990) Agarwal et al. (1994) Referências Thresiamma et al. (1996)…...
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09 Jan 2018
TL;DR: Indian medicinal plants/, Indian medicinal plants /, مرکز فناوری اطلاعات و اصاع رسانی, کδاوρزی
Abstract: Indian medicinal plants / , Indian medicinal plants / , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
8,252 citations
831 citations
Journal Article•
TL;DR: Investigation of the chemopreventive action of dietary curcumin on azoxymethane-induced colon carcinogenesis and also the modulating effect of this agent on the colonic mucosal and tumor phospholipase A2, phospholIPase C gamma 1, lipoxygenase, and cyclo oxygengenase activities in male F344 rats indicates that dietary administration ofCurcumin significantly inhibited incidence of colon adenocarcinomas.
Abstract: Human epidemiological and laboratory animal model studies have suggested that nonsteroidal antiinflammatory drugs reduce the risk of development of colon cancer and that the inhibition of colon carcinogenesis is mediated through the alteration in cyclooxygenase metabolism of arachidonic acid. Curcumin, which is a naturally occurring compound, is present in turmeric, possesses both antiinflammatory and antioxidant properties, and has been tested for its chemopreventive properties in skin and forestomach carcinogenesis. The present study was designed to investigate the chemopreventive action of dietary curcumin on azozymethaneinduced colon carcinogenesis and also the modulating effect of this agent on the colonic mucosal and tumor phospholipase A 2 , phospholipase Cγ1, lipoxygenase, and cyclooxygenase activities in male F344 rats. At 5 weeks of age, groups of animals were fed the control (modified AIN-76A) diet or a diet containing 2000 ppm of curcumin. At 7 weeks of age, all animals, except those in the vehicle (normal saline)-treated groups, were given two weekly s.c. injections of azoxymethane at a dose rate of 15 mg/kg body weight. All groups were continued on their respective dietary regimen until the termination of the experiment at 52 weeks after the carcinogen treatment. Colonic tumors were evaluated histopathologically. Colonic mucosa and tumors were analyzed for phospholipase A 2 , phospholipase Cγ1, ex vivo prostaglandin (PG) E 2 , cyclooxygenase, and lipoxygenase activities. The results indicate that dietary administration of curcumin significantly inhibited incidence of colon adenocarcinomas ( P P P P 57% compared to the control diet. Animals fed the curcumin diet showed decreased activities of colonic mucosal and tumor phospholipase A 2 (50%) and phospholipase Cγ1 (40%) and levels of PGE 2 (>38%). The formation of prostaglandins such as PGE 2 , PGF 2α , PGD 2 , 6-keto PGF 1α , and thromboxane B 2 through the cyclooxygenase system and production of 5( S )-, 8( S )-, 12( S )-, and 15( S )-hydroxyeicosatetraenoic acids via the lipoxygenase pathway from arachidonic acid were reduced in colonic mucosa and tumors of animals fed the curcumin diet as compared to control diet. Although the precise mechanism by which curcumin inhibits colon tumorigenesis remains to be elucidated, it is likely that the chemopreventive action, at least in part, may be related to the modulation of arachidonic acid metabolism.
706 citations
TL;DR: Salmonella/microsome tests (Ames tests) and chromosomal aberration tests in vitro using a Chinese hamster fibroblast cell line were carried out on 190 synthetic food additives and 52 food additives derived from natural sources, all of which are currently used in Japan.
634 citations