Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins.
25 Mar 1971-Journal of Biological Chemistry (American Society for Biochemistry and Molecular Biology)-Vol. 246, Iss: 6, pp 1889-1894
TL;DR: Evidence is presented to establish the identity of the radioactive derivative as 5-acetamido-3, 5-dideoxy-l-arabino-2-heptulosonic acid and to indicate it as the sole site of tritium incorporation in the carbohydrate chain.
About: This article is published in Journal of Biological Chemistry.The article was published on 1971-03-25 and is currently open access. It has received 479 citations till now. The article focuses on the topics: Sialic acid.
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1,066Â citations
TL;DR: Mild periodate oxidation can prove useful during the early stages of hybridoma screening in order to select for or against anti-carbohydrate antibodies.
738Â citations
TL;DR: The purification, by affinity chromatography, of an hepatic protein which retains the characteristic binding properties associated with the membranes is described, which indicates a high degree of aggregation in the final, water-soluble preparation.
555Â citations
TL;DR: Siglecs as mentioned in this paper is a family of I-type lectins with sialic acid (Sia)-binding properties and characteristic amino-terminal structural features, which appear to have evolved by convergent evolution.
Abstract: Animal glycan-recognizing proteins can be broadly classified into two groups-lectins (which typically contain an evolutionarily conserved carbohydrate-recognition domain [CRD]) and sulfated glycosaminoglycan (SGAG)-binding proteins (which appear to have evolved by convergent evolution). Proteins other than antibodies and T-cell receptors that mediate glycan recognition via immunoglobulin (Ig)-like domains are called "I-type lectins." The major homologous subfamily of I-type lectins with sialic acid (Sia)-binding properties and characteristic amino-terminal structural features are called the "Siglecs" (Sia-recognizing Ig-superfamily lectins). The Siglecs can be divided into two groups: an evolutionarily conserved subgroup (Siglecs-1, -2, and -4) and a CD33/Siglec-3-related subgroup (Siglecs-3 and -5-13 in primates), which appear to be rapidly evolving. This article provides an overview of historical and current information about the Siglecs.
499Â citations
TL;DR: Recent developments in the molecular biology of AMD are reviewed, including single nucleotide polymorphisms within the Factor H gene, which may predispose individuals to the susceptibility of AMD as well as single nucleophile polymorphisms that may confer a protective effect.
497Â citations
References
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TL;DR: The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated.
Abstract: A simple and rapid method is presented for the preparation of I/sup 131/- labeled human growth hormone of high specific radioactivity (240-300 mu C/ mu g). Low amounts of carrierfree I/sup 131/ iodide (2 mC) are allowed to react, without prior treatment, with small quantities of protein (5 mu g) in a highyield reaction (approx. 70% transfer of I/sup 131/ to protein). The degree of chemical substitution is minimized (0.5- 1.0 atom of iodine/molecule of protein) by the use of carrier-free I/sup 131/ iodide. The I/sup 131/-labeled hormone (up to 300 mu C/ mu g) contains no detectable degradation products and is immunologically identical with the unlabeled hormone. The loss of immunological reactivity at high specific radioactivities or at high levels of chemical substitution with STAI/sup 127/!iodine is demonstrated. (auth)
10,047Â citations
TL;DR: This chapter discusses the different aspects of thiobarbituric acid assay of sialic acid, which is suitable for measuring the release of bound sialoic acid by sialidase and hydrolysis of sIALic acid-containing material must be carried out for the measurement of total sialsic acids.
6,264Â citations
TL;DR: A new method for the quantitive determination of sialic acids is described, which is about 50% more sensitive than the orcinol-hydrochloric acid method generally used and considerably lower with the resorcin reagent.
2,741Â citations
1,686Â citations
TL;DR: Evidence is presented to indicate a generalized role for the terminal sialic acid residues of circulating glycoproteins of desialylated plasma proteins inducers of gonadotropic hormones and follicle-stimulating hormone.
1,036Â citations