Suaeda japonica Makino Attenuates Lipopolysaccharide- Induced Neuro-Inflammatory Responses in BV-2 Microglia via NF-kappa B Signaling
Reads0
Chats0
TLDR
The findings imply that SJE may be a potential therapeutic agent in regulating microgliamediated neuroinflammatory responses observed in several neurodegenerative diseases.Abstract:
Purpose: To evaluate in vitro anti-oxidant and anti-neuro-inflammatory activities of Suaeda japonica extract (SJE) in lipopolysaccharide (LPS)-stimulated BV-2 microglial cells. Methods: 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging assay was used to study the antioxidant effects. 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyl-tetrazolium bromide (MTT) assay was used to study cell viabilities. LPS-stimulated BV- microglial cells were used to study the gene expression and production of inflammatory mediators determined by Western blot analysis. Results: SJE significantly inhibited the DPPH generated free radicals, and suppressed LPS-induced expression of inducible nitric oxide synthase (iNOS) and production of nitric oxide (NO) in a concentration-dependent manner. It decreased LPS-induced expression of some inflammatory mediators and pro-inflammatory cytokines (cyclooxygenase-2 and interleukin (IL)-6). This suppression of inflammatory mediators was nuclear factor kappa-B (NF-κB)-dependent. Conclusion: Our findings imply that SJE may be a potential therapeutic agent in regulating microgliamediated neuroinflammatory responses observed in several neurodegenerative diseases.read more
Citations
More filters
Journal ArticleDOI
Antioxidant and Anti-inflammatory Activity of Six Halophytes in Korea
TL;DR: The aim of this study was to measure and compare polyphenol content, antioxidant, and anti-inflammatory activity of six halophytes (Limonium tetragonum, Suaeda glauca,Suaeda japonica, Salicornia europaea, Triglochin maritimum, and Sonchus brachyotus).
Journal ArticleDOI
Genus Suaeda: Advances in Phytology, Chemistry, Pharmacology and Clinical Application (1895 - 2021).
TL;DR: More than 100 species of annual herb genus Suaeda widely distribute (Asia, North America, northern Africa and Europe), are rich in resources (about hundreds of millions of tons/Y) and have a long historical application as mentioned in this paper .
Journal ArticleDOI
Comparison of the Biological Activities of Electrodialysis-desalted Bioactive Compounds from the Halophyte Suaeda japonica
TL;DR: It is claimed that 2,000 species of flowering plants in the world have been classified as “threatened” by the European Union in recent years.
References
More filters
Journal ArticleDOI
Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids
Laura C. Green,David A. Wagner,Joseph A. Glogowski,Paul L. Skipper,John S. Wishnok,Steven R. Tannenbaum +5 more
TL;DR: A new automated system for the analysis of nitrate via reduction with a high-pressure cadmium column that automatically eliminates interference from other compounds normally present in urine and other biological fluids is described.
Journal ArticleDOI
Role of transcription factor NF-kappa B/Rel in induction of nitric oxide synthase.
TL;DR: Reporter constructs containing truncated promoter regions revealed that activation of NF-kappa B/Rel is critical in the induction of iNOS by LPS, however, additional, newly synthesized proteins contribute to the NF- kappa Bd-dependent transcription factor complex on the iN OS promoter in LPS-treated mouse macrophages.
Journal ArticleDOI
The mechanism of salt tolerance in halophytes
TL;DR: The objective was to establish an experimental procedure and show direct AFM measurements that unequivocally can be assigned as a mode of action for the conversion of NalK to Na6(SO4)(SO3) during the cycling process.
Journal ArticleDOI
Role of Microglia in Inflammation-Mediated Neurodegenerative Diseases: Mechanisms and Strategies for Therapeutic Intervention
Bin Liu,Jau-Shyong Hong +1 more
TL;DR: Recent advances on the study of the role of microglia based on findings from animal and cell culture models in the pathogenesis of neurodegenerative diseases, with particular emphasis on Parkinson's disease are summarized.
Journal ArticleDOI
Macrophages and microglia in the nervous system
V. Hugh Perry,Siamon Gordon +1 more
TL;DR: The possible functions of resident and recruited macrophages in the developing and adult nervous system are reviewed and what contribution these cells might make to repair mechanisms in the central and peripheral nervous systems are examined.