Substrate-inducible Microsomal Aryl Hydroxylase in Mammalian Cell Culture: I. Assay and properties of induced enzyme
Reads0
Chats0
TLDR
The spectrophotofluorometric determination of hydroxylated benzo[a]pyrene products is sufficiently sensitive to detect 10-12 mole per ml and has great utility in measuring the hydroxymatic activity of cells grown in culture.About:
This article is published in Journal of Biological Chemistry.The article was published on 1968-12-10 and is currently open access. It has received 1664 citations till now. The article focuses on the topics: Enzyme inducer & Benzopyrene Hydroxylase.read more
Citations
More filters
Journal ArticleDOI
Role of Aryl Hydrocarbon Receptor-mediated Induction of the CYP1 Enzymes in Environmental Toxicity and Cancer
TL;DR: Differences in AHR affinity between inbred mouse strains reflect variations in CYP1 inducibility and clearly have been shown to be associated with differences in risk of toxicity or cancer caused by PAHs and arylamines.
Book ChapterDOI
Human Cytochrome P450 Enzymes
TL;DR: Human P450s 1A1,6 1A2,7 2A6,8 2C8,9 2C9,9,10 2D67,11,12 and lanosterol 14α-demethylase18 were isolated in this general manner.
Book ChapterDOI
Epoxides in polycyclic aromatic hydrocarbon metabolism and carcinogenesis.
P. Sims,P.L. Grover +1 more
TL;DR: This chapter discusses the role of intermediates such as free radicals, radical cations, and carbonium ions in polycyclic hydrocarbon carcinogenesis, and discusses the factors that can influence the formation, further metabolism, and biological effectiveness of polycyclIC hydrocarbon epoxides.
Journal ArticleDOI
Separation and characterization of highly purified forms of liver microsomal cytochrome P-450 from rats treated with polychlorinated biphenyls, phenobarbital, and 3-methylcholanthrene.
TL;DR: The forms of cytochrome P-450 obtained from phenobarbitaland 3-methylcholanthrene-treated rats are indistinguishable from the corresponding forms from Aroclor 1254~treated rats by all criteria examined.
Journal ArticleDOI
Cytochrome P-450 hPCN3, a novel cytochrome P-450 IIIA gene product that is differentially expressed in adult human liver. cDNA and deduced amino acid sequence and distinct specificities of cDNA-expressed hPCN1 and hPCN3 for the metabolism of steroid hormones and cyclosporine.
Toshifumi Aoyama,Shigeru Yamano,David J. Waxman,D. P. Lapenson,Urs A. Meyer,V Fischer,Rachel F. Tyndale,T Inaba,W Kalow,Harry V. Gelboin,Frank J. Gonzalez +10 more
TL;DR: HPCN3 is a newly described cytochrome P-450 that is differentially expressed in the adult human population and that has overlapping substrate specificity compared to hPCN1 for metabolism of steroid and drug substrates.
References
More filters
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal ArticleDOI
The Carbon Monoxide-binding Pigment of Liver Microsomes II. SOLUBILIZATION, PURIFICATION, AND PROPERTIES
Tsuneo Omura,Ryo Sato +1 more
TL;DR: The present paper gives a detailed account of the investigations on rabbit liver microsomes and crude microsomal digests, which have led to postulate the hemoprotein nature of the pigment.
Journal Article
Pharmacological implications of microsomal enzyme induction
TL;DR: It is of considerable interest that certain inducers of liver microsomal enzymes have recently been used therapeutically for the treatment of hyperbilirubinemia in jaundiced children and for thetreatment of Cushing's syndrome.
Journal ArticleDOI
Microsomal triphosphopyridine nucleotide-cytochrome c reductase of liver.
Charles Williams,Henry Kamin +1 more
TL;DR: The biological role of reduced triphosphopyridine nucleotide (TPNH) and the metabolic pathways of its hydrogen atom and electron appear to be fundamentally different from those of reduced diphosphipyridineucleotide (DPNH).
Related Papers (5)
The Carbon Monoxide-binding Pigment of Liver Microsomes I. EVIDENCE FOR ITS HEMOPROTEIN NATURE
Tsuneo Omura,Ryo Sato +1 more