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Open accessJournal ArticleDOI: 10.1038/S41598-021-84660-5

Supplementation of tuna hydrolysate and insect larvae improves fishmeal replacement efficacy of poultry by-product in Lates calcarifer (Bloch, 1790) juveniles.

02 Mar 2021-Scientific Reports (Nature Publishing Group)-Vol. 11, Iss: 1, pp 4997-4997
Abstract: The effects of feeding different levels of poultry by-product meal (PBM) replacing fishmeal (FM) protein, supplemented with tuna hydrolysate (TH) and Hermetia illucens (HI) larvae, on the growth, fillet quality, histological traits, immune status, oxidative biomarker levels and gut microbiota of juvenile barramundi, Lates calcarifer were investigated for six weeks. Barramundi were fed four isonitrogenous and isolipidic diets in which a FM based diet was used as the Control diet (Diet1) and compared with other non-FM diets containing 80%, 85% and 90% PBM along with the concurrent supplementation of 5% and/or 10% TH and HI larvae meal. These treatment diets were designated as 80PBM10TH+10HI (Diet2), 85PBM5TH+10HI (Diet3) and 90PBM5TH+5HI (Diet4). The growth and condition factor of fish fed 80PBM10TH+10HI and 85PBM5TH+10HI were significantly higher than the Control. Total saturated, monounsaturated and polyunsaturated fatty acid retention in the fish muscle increased in fish fed PBM-based diets, supplemented with TH and HI larvae meal, with no adverse effect on post-harvest characteristics such as texture and colour of fish fillets. Improvement in serum total bilirubin and total protein content was found in all fish fed TH and HI larvae supplemented PBM. Similarly, immune response showed a significant increase in fish fed non-FM test diets than the Control. In the distal intestine, supplementation of any quantities of TH and HI larvae to PBM led to an increase in the microvilli density and neutral mucins while the number of goblet cells in the skin were unchanged. Liver, kidney, and spleen histology demonstrated a normal structure with no obvious changes in response to all test diets. Bacterial diversity increased in fish fed Diets 2 and 3 with a high abundance of Proteobacteria in Diets 1 and 4 and Firmicutes in Diets 2 and 3. The fish on test diets showed a lower abundance of genus Vibrio. Fish fed TH and HI larvae supplemented PBM diets showed lower infection rate to V. harveyi than the Control. Collectively, concurrent supplementation of TH and HI larvae could improve the quality of PBM diets with positive effects on growth, fillet quality, intestinal health, immunity, and disease resistance.

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Topics: Fish meal (61%)
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7 results found


Journal ArticleDOI: 10.1016/J.AQUACULTURE.2021.737026
15 Oct 2021-Aquaculture
Abstract: Poultry by-product meal (PBM) as a replacement for fish meal (FM), an expensive and unsustainable protein aquafeed ingredient, has been tested on different aquaculture fish species. However, the complete replacement of FM with a mixture of PBM and Hermetia illucens (HI) larvae in barramundi culture has not been previously investigated. In this study, results are presented on growth performance, fillet fatty acid composition, serum metabolites, skin mucosal barriers, hepatic steatosis, antioxidant activity, and immunity of juvenile barramundi, Lates calcarifer fed either a FM-based diet (0PBM-0HI) or two test diets in which total FM protein was replaced by a mixture of 70% PBM and 30% full-fat (FHI) and defatted HI larvae (DHI) meal (designated as 70PBM-30FHI and 70PBM-30DHI). After 56 days of feeding, the results showed that the growth was affected when fish were fed 70PBM-30DHI with a higher feed conversion ratio (FCR) with respect to 0PBM-0HI and 70PBM-30FHI diets. There was no variation in growth performance, feed utilization, and FCR between 0PBM-0HI and 70PBM-30FHI. The retention of total saturated and monounsaturated fatty acids increased in the fillet of juveniles fed 70PBM-30FHI and 70PBM-30DHI while total retention of polyunsaturated (PUFA), n-3 PUFA, and n-6 PUFA decreased than the control. Serum alanine transaminase (ALT) increased in fish fed 70PBM-30DHI whereas haptoglobin upsurged in 70PBM-30FHI compared to 0PBM-0HI. There were no significant effects in the serum immune response between dietary treatments whilst serum and liver CAT activity were negatively impacted by 70PBM-30DHI. The 70PBM-30DHI induced hepatic steatosis whilst 0PBM-0HI and 70PBM-30FHI showed no obvious change in the liver. Skin mucosal barriers were impacted by 70PBM-30DHI whilst 70PBM-30FHI fed fish showed a similar response to 0PBM-0HI. The fish fed on 70PBM-30DHI showed a higher proportion of infection rate with Vibrio harveyi than 70PBM-30FHI but showed no variation with the control-fed fish. In summary, FHI larvae meal could be a good complementary protein source, particularly when replacing FM completely with insect-based proteins.

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Topics: Fish meal (64%), Poultry by-product meal (56%), Feed conversion ratio (53%) ... show more

2 Citations


Journal ArticleDOI: 10.1016/J.AQUACULTURE.2021.737346
15 Jan 2022-Aquaculture
Abstract: This study was conducted to investigate the combined effects of probiotic yeast, Saccharomyces cerevisiae, and lactic acid bacteria, Lactobacillus casei on the growth performance, biochemical response, cytokine gene expression, gut histomorphology and microbial composition of juvenile barramundi, Lates calcarifer. Barramundi juveniles with an initial mean weight of 12.17 ± 0.15 g were randomly allocated into 8 tanks (300L) at a density of 20 fish per tank and fed either a basal diet (control), or the same basal diet supplemented with 1% of S. cerevisiae and 1% of L. casei, collectively termed ‘probiotic’, for 56 days in quadruplicate. Results indicate that dietary probiotic supplementation produced significant (P

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Topics: Lactobacillus casei (60%), Probiotic (52%)

1 Citations


Open accessJournal ArticleDOI: 10.1016/J.AQREP.2021.100875
Hung Duc Pham1, Muhammad A. B. Siddik2, Ut Van Phan1, Hoang Minh Le1  +1 moreInstitutions (2)
Abstract: Six experimental diets were prepared in a 3 × 2 experimental design involving three inclusion levels (0%, 50%, and 100%) of poultry by-product meal (PB) and two concentrations (0 and 60 mg/kg) of tuna hydrolysates (TH), labelled as PB0, PB0TH, PB50, PB50TH, PB100, and PB100TH, respectively. The diets were fed to juvenile pompano for 10 weeks to investigate the nutritional value of PB in conjunction with TH supplementation on growth, feed efficiency, biochemical composition, haematological and histological responses in the species. The results indicated that the final body weight, specific growth rate, and feed conversion ratio were negatively affected by the increasing replacement of fishmeal by dietary poultry by-product meal with or without TH supplementation compared to the control (P

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Topics: Poultry by-product meal (62%), Pompano (56%), Fish meal (56%) ... show more

1 Citations


Open accessJournal ArticleDOI: 10.1016/J.AQUACULTURE.2021.737760
26 Nov 2021-Aquaculture
Abstract: Replacement of fishmeal (FM) from aquafeed has gained tremendous attention over the past 20 years due to environmental and ecological ill-impacts of large-scale capture of wild fishes. Insects, black soldier fly (BSF) possess balanced nutritional properties, hence, considered as an ideal candidate for the replacement of FM. A number of studies have been performed on the effects of BSF as a fishmeal alternative on the gut microbiota of aquatic animals using high throughput sequencing (HTS). However, due to the inconsistency in the success of BSF inclusions, a systematic meta-analysis approach was imperative to conclude the usefulness of BSF inclusion in aquafeed. In this context, the present study systematically analysed the HTS data obtained from gut microbiota of BSF, and rainbow trout (RT) fed BSF from various life cycles with different formulations. Results revealed that Actinobacteria and Firmicutes were most abundant in the gut of BSF and fish fed BSF diets while Proteobacteria was the dominant bacteria in the fish gut fed FM. The augmentation of Actinobacteria and Firmicutes in the fish gut was mainly due to higher abundance of Actinomyces, Bacillus, Lactobacillus and Enterococcus at the genus level. More diversified gut microbiota was observed in fish fed larvae and pre-pupae BSF, while defatted BSF was linked to a specific group of bacteria. Feeding BSF was shown to reduce the core microbiota of rainbow trout by increasing Firmicutes. Overall, the findings of this study could be used as future references for fish and shellfish nutrition, specifically in formulating aquafeed with BSF incorporation.

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Topics: Firmicutes (54%), Fish meal (51%)

Journal ArticleDOI: 10.1016/J.SCITOTENV.2021.149045
Abstract: Promoting a circular economy via the transformation of food waste into alternative and high-value protein sources for aquaculture diets is a novel approach to developing alternative raw materials to fishmeal (FM). This approach can reduce the ecological impact on the aquatic environment and simultaneously can provide an option for sustainable food waste management. In this context, we report a 56-day trial of feeding barramundi, Lates calcarifer on four iso‑nitrogenous and iso-lipidic diets where the control (0PBM-0HI) was a FM-based diet and the other test diets replaced FM protein with mixtures of a poultry by-product meal (PBM) and a full-fat Hermetia illucens (HI) larvae meal reared on fish waste: the test diets were 85% PBM + 15% HI (85PBM-15HI), 80% PBM + 20% HI (80PBM-20HI) and 75% PBM + 25% HI (75PBM-25HI). Fish fed PBM-HI-based diets showed an equal growth rate and amino acid profile when compared to the control group. Among all serum metabolites, alanine aminotransferase and glutamate dehydrogenase decreased in fish fed PBM-HI-based diets, whilst total protein levels improved in the same diets. Serum lysozyme and bactericidal activity were unchanged which supported the observation of similar infection rates against V. harveyi. Except for the kidney and intestine, catalase activity in the serum and liver increased in fish-fed PBM-HI-based diets. In assessing the gastrointestinal mucosal morphology, the goblet cells producing neutral mucins were higher in PBM-HI-fed fish than the control. PBM-HI diets also enhanced bacterial richness and diversity and increased abundance for Lactobacillus, Clostridium, and Ruminococcus. In summary, combining full-fat HI with PBM allowed complete replacement of FM with no negative effects on growth whilst improving gut health. Such diets would be beneficial for the aquaculture industry, both ecologically and economically, as well as providing value-adding to animal waste as alternative protein sources for aquafeed production.

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Topics: Fish meal (58%), Hermetia illucens (53%)

References
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140 results found


Open accessJournal ArticleDOI: 10.1038/NMETH.F.303
11 Apr 2010-Nature Methods
Abstract: Supplementary Figure 1 Overview of the analysis pipeline. Supplementary Table 1 Details of conventionally raised and conventionalized mouse samples. Supplementary Discussion Expanded discussion of QIIME analyses presented in the main text; Sequencing of 16S rRNA gene amplicons; QIIME analysis notes; Expanded Figure 1 legend; Links to raw data and processed output from the runs with and without denoising.

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24,116 Citations


Open accessJournal ArticleDOI: 10.1093/NAR/GKS1219
Christian Quast1, Elmar Pruesse2, Pelin Yilmaz2, Jan Gerken2  +4 moreInstitutions (2)
Abstract: SILVA (from Latin silva, forest, http://www.arb-silva.de) is a comprehensive web resource for up to date, quality-controlled databases of aligned ribosomal RNA (rRNA) gene sequences from the Bacteria, Archaea and Eukaryota domains and supplementary online services. The referred database release 111 (July 2012) contains 3 194 778 small subunit and 288 717 large subunit rRNA gene sequences. Since the initial description of the project, substantial new features have been introduced, including advanced quality control procedures, an improved rRNA gene aligner, online tools for probe and primer evaluation and optimized browsing, searching and downloading on the website. Furthermore, the extensively curated SILVA taxonomy and the new non-redundant SILVA datasets provide an ideal reference for high-throughput classification of data from next-generation sequencing approaches.

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11,937 Citations


Open accessJournal ArticleDOI: 10.1371/JOURNAL.PONE.0009490
10 Mar 2010-PLOS ONE
Abstract: Background We recently described FastTree, a tool for inferring phylogenies for alignments with up to hundreds of thousands of sequences. Here, we describe improvements to FastTree that improve its accuracy without sacrificing scalability.

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7,488 Citations


Open accessJournal ArticleDOI: 10.1371/JOURNAL.PONE.0061217
Paul J. McMurdie1, Susan Holmes1Institutions (1)
22 Apr 2013-PLOS ONE
Abstract: Background The analysis of microbial communities through DNA sequencing brings many challenges: the integration of different types of data with methods from ecology, genetics, phylogenetics, multivariate statistics, visualization and testing. With the increased breadth of experimental designs now being pursued, project-specific statistical analyses are often needed, and these analyses are often difficult (or impossible) for peer researchers to independently reproduce. The vast majority of the requisite tools for performing these analyses reproducibly are already implemented in R and its extensions (packages), but with limited support for high throughput microbiome census data. Results Here we describe a software project, phyloseq, dedicated to the object-oriented representation and analysis of microbiome census data in R. It supports importing data from a variety of common formats, as well as many analysis techniques. These include calibration, filtering, subsetting, agglomeration, multi-table comparisons, diversity analysis, parallelized Fast UniFrac, ordination methods, and production of publication-quality graphics; all in a manner that is easy to document, share, and modify. We show how to apply functions from other R packages to phyloseq-represented data, illustrating the availability of a large number of open source analysis techniques. We discuss the use of phyloseq with tools for reproducible research, a practice common in other fields but still rare in the analysis of highly parallel microbiome census data. We have made available all of the materials necessary to completely reproduce the analysis and figures included in this article, an example of best practices for reproducible research. Conclusions The phyloseq project for R is a new open-source software package, freely available on the web from both GitHub and Bioconductor.

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Topics: Bioconductor (53%)

7,065 Citations


Open accessJournal ArticleDOI: 10.1186/GB-2011-12-6-R60
Nicola Segata1, Jacques Izard1, Jacques Izard2, Levi Waldron1  +4 moreInstitutions (3)
24 Jun 2011-Genome Biology
Abstract: This study describes and validates a new method for metagenomic biomarker discovery by way of class comparison, tests of biological consistency and effect size estimation. This addresses the challenge of finding organisms, genes, or pathways that consistently explain the differences between two or more microbial communities, which is a central problem to the study of metagenomics. We extensively validate our method on several microbiomes and a convenient online interface for the method is provided at http://huttenhower.sph.harvard.edu/lefse/.

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Topics: Biomarker discovery (51%), Metagenomics (51%)

6,049 Citations