Suppression of NF-κB p65 nuclear translocation and tumor necrosis factor-α by Pongamia pinnata seed extract in adjuvant-induced arthritis.
TL;DR: Exposure to PSE significantly decreased paw diameter, tissue myeloperoxidase level, and levels of urinary HP and glucosamine, as well as of serum TNFα and IL-10 in adjuvant-injected (arthritic) rats, suggesting PSE appears to be able to prevent experimental arthritis.
Abstract: Pongamia pinnata is a plant known for its therapeutic usage in Indian traditional medicine. Despite the controversy regarding toxic flavonoid and erucic acid content, the seed of this plant is consumed in tribal medicine and its oil is used in Ayurveda to treat psoriasis and arthritis. This study explored the potential anti-arthritic effects of a P. pinnata seed (hexane) extract (PSE) at non-lethal doses in an adjuvant-induced arthritic rat model; possible mechanisms of any observed effects were also explored. After establishing the lethal doses arising from oral exposure to the extract, the material was administered per os daily at two doses (0.3 g/kg/day; 0.5 g/kg/day) to arthritic rats. Other rats received indomethacin or vehicle (control). Treatments were performed for a total of 14 days. One day after the final exposure, the rats were euthanized to permit harvest of various cells, blood, and tissues for analyses. Paw diameter and tissue myeloperoxidase activity in the paws were evaluated as i...
Citations
More filters
Journal Article•
TL;DR: Excerpt Essentially all the hydroxyproline in the tissues of vertebrates is found in collagen, and, except for the small amount of hydroxy Proline in elastin, no other animal protein contains signif...
Abstract: Excerpt Essentially all the hydroxyproline in the tissues of vertebrates is found in collagen, and, except for the small amount of hydroxyproline in elastin, no other animal protein contains signif...
244 citations
TL;DR: The results indicate that EG extract and fractions significantly attenuated adjuvant arthritis in rats by decreasing the levels of aforementioned pro-inflammatory and increasing the Levels of anti-inflammatory mediators, suggesting that Ephedra gerardiana aerial parts might be used as a therapeutic agent for treating human arthritis.
50 citations
TL;DR: Results showed that RO extract and fractions significantly reduced protein denaturation and protected erythrocyte membrane in concentration dependent manner, which suggests that Ribes orientale roots may be used as a therapeutic agent for treating human arthritis.
28 citations
30 Jul 2020
TL;DR: The extract significantly subdued paw swelling and arthritic scoring, inhibited cachexia, and considerably improved biochemical and hematological modifications, supporting its folkloric use for treating rheumatoid arthritis.
Abstract: Alternanthera bettzickiana is being used as a folk remedy for treating arthritis by conventional healers in Thailand. The current research was undertaken to explore the antiarthritic potential of A. bettzickiana ethanolic extract (ABEE). Plant characterization, molecular docking, and in vitro and in vivo (ABEE at 250, 500, and 1000 mg/kg was administered orally to rats once daily for 28 days) studies to explore the antiarthritic effect and enzyme-linked immunosorbent assay (ELISA) and real-time polymerase chain reaction (RT-PCR) analyses were performed. Oxidative stress biomarkers (superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA)) in the serum and histopathological and radiographic assessment of joints were also carried out. Gallic acid, catechin, chlorogenic acid, sinapic acid, quercetin, and γ- and α-tocopherol were identified in high-performance liquid chromatography (HPLC). Molecular docking revealed a strong interaction between these compounds and cyclooxygenase (COX) enzymes. The extract significantly subdued paw swelling and arthritic scoring, inhibited cachexia, and considerably improved biochemical and hematological modifications. SOD and CAT levels increased and the MDA level decreased in ABEE-treated rats dose-dependently. Radiographic and histopathological analyses also supported the antiarthritic effect of ABEE, which was linked with the downregulation of nuclear factor (NF)-kB, COX-2, interleukin (IL)-6, tumour necrosis factor (TNF)-α, and IL-1β and upregulation of IL-10, I-kB, and IL-4 as compared to disease control rats. Results suggested that A. bettzickiana possessed antiarthritic potential, supporting its folkloric use for treating rheumatoid arthritis.
25 citations
TL;DR: Bellamya bengalensis, traditionally used against diseases with underlying etiology of cell mediated immunity as in rheumatism, is shown to inhibit ROS, NO, and TNFα production along with NF-kB p65 nuclear translocation in LPS stimulated macrophage.
22 citations
References
More filters
Journal Article•
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
289,852 citations
"Suppression of NF-κB p65 nuclear tr..." refers methods in this paper
...Protein levels in each homogenate were determined in parallel using the method of Lowry et al. (1951)....
[...]
01 Mar 1948
TL;DR: The method provides means for the rapid test of parallelism of two curves and easy computation of relative potency with its confidence limits and its accuracy is commensurate with the nature of dose-per cent effect data.
Abstract: 1. A rapid graphic method for approximating the Median Effective Dose and the Slope of dose-per cent effect curves is presented. Confidence limits of both of these parameters for 19/20 probability are given by the method. In addition, confidence limits for any other probability or for a dose other than the Median Effective Dose are readily estimated. 2. The data are used throughout the method in their original form without transformation to logarithms and probits. 3. An effective means for plotting and using 0 and 100 per cent effects is provided. 4. The calculations have been simplified by means of nomographs to the extent that a slide rule is a convenience but not a necessity. 5. A simple means is provided for detecting a poorly fitted line or significantly heterogeneous data, In the former case, the line may be refitted; in the latter, the confidence limits are corrected for the degree of heterogeneity. 6. The method provides means for the rapid test of parallelism of two curves and easy computation of relative potency with its confidence limits. 7. Although the method is rapid (10-15 minutes), its accuracy is commensurate with the nature of dose-per cent effect data.
7,221 citations
"Suppression of NF-κB p65 nuclear tr..." refers methods in this paper
...Minimum lethal dose determination and general toxicity of PSE Minimum Lethal Dose (MLD) determination in the rats was performed according to the methods of Litchfield and Wilcoxon (1949)....
[...]
Journal Article•
TL;DR: In this article, a rapid graphic method for approximating the median effective dose and the slope of dose-per-cent effect curves is presented, and confidence limits of both of these parameters for 19/20 probability are given by the method.
Abstract: 1. A rapid graphic method for approximating the Median Effective Dose and the Slope of dose-per cent effect curves is presented. Confidence limits of both of these parameters for 19/20 probability are given by the method. In addition, confidence limits for any other probability or for a dose other than the Median Effective Dose are readily estimated. 2. The data are used throughout the method in their original form without transformation to logarithms and probits. 3. An effective means for plotting and using 0 and 100 per cent effects is provided. 4. The calculations have been simplified by means of nomographs to the extent that a slide rule is a convenience but not a necessity. 5. A simple means is provided for detecting a poorly fitted line or significantly heterogeneous data, In the former case, the line may be refitted; in the latter, the confidence limits are corrected for the degree of heterogeneity. 6. The method provides means for the rapid test of parallelism of two curves and easy computation of relative potency with its confidence limits. 7. Although the method is rapid (10-15 minutes), its accuracy is commensurate with the nature of dose-per cent effect data.
7,217 citations
TL;DR: It is demonstrated that MPO can be used as a marker for skin neutrophil content: it is recoverable from skin in soluble form, and is directly related to neutrophIL number.
3,298 citations
"Suppression of NF-κB p65 nuclear tr..." refers methods in this paper
...The activity of tissue MPO was assessed using the method of Bradley et al. (1982)....
[...]
TL;DR: A critical review is given of hydroxyproline analysis, finding a procedure which is faster and more sensitive than the former one and as reliable.
1,731 citations
"Suppression of NF-κB p65 nuclear tr..." refers methods in this paper
...Urinary hydroxyproline (HP) concentration was measured according to Neuman and Logan (1950) and expressed in terms of mg hydroxyproline/day....
[...]