Surpassing the lateral resolution limit by a factor of two using structured illumination microscopy.
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...3D structural illuminationmicroscopy (3D-SIM), a technique enabling 2X the resolution of light microscopy (Gustafsson, 2000), was used to assess the P5 dLGN of CX3CR1+/EGFP mice immunolabeled for VGlut2....
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...Periodic illumination patterns can be created through the interference of multiple light sources in the axial direction (18), the lateral direction (19), or both (20)....
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References
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"Surpassing the lateral resolution l..." refers background in this paper
...Although some recent non-linear concepts show promise (Klar & Hell, 1999), only one established technique has the ability to go beyond the limit in principle, namely confocal fluorescence microscopey (Minsky, 1961; Pawley, 1995)....
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"Surpassing the lateral resolution l..." refers background or methods in this paper
...In fact, the resolution performance of wide-field microscopes in the third dimension can itself be enhanced by laterally structured illumination similar to that used here (Neil et al., 1997; Neil et al., 1998)....
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...The last approach can enhance the axial sectioning ability further by making the illumination vary axially as well as laterally 2 this effect is similar to that of illuminating the coarser grating with fully incoherent light (Neil et al., 1997), but maintains the maximum lateral resolution enhancement and strong pattern contrast....
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...…axial sectioning ability further by making the illumination vary axially as well as laterally 2 this effect is similar to that of illuminating the coarser grating with fully incoherent light (Neil et al., 1997), but maintains the maximum lateral resolution enhancement and strong pattern contrast....
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789 citations
"Surpassing the lateral resolution l..." refers background in this paper
...Although some recent non-linear concepts show promise (Klar & Hell, 1999), only one established technique has the ability to go beyond the limit in principle, namely confocal fluorescence microscopey (Minsky, 1961; Pawley, 1995)....
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702 citations
"Surpassing the lateral resolution l..." refers background in this paper
...It is known that non-linear, constrained deconvolution algorithms can enhance apparent resolution by making use of a priori information about the sample, such as the fact that the dye density is non-negative (Agard et al., 1989; Carrington et al., 1995)....
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