Sweet sensation: Developing a single-cell fluorescent reporter of glycolytic heterogeneity
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Ortega et al. as discussed by the authors constructed RNA sensors of fructose-6-bisphosphate (FBP) that report on metabolite levels within single yeast cells, using RNA sequencing.About:
This article is published in Chemistry & Biology.The article was published on 2021-11-18 and is currently open access. It has received 0 citations till now. The article focuses on the topics: Aptamer & Chemical biology.read more
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Fluorescence Imaging of Cellular Metabolites with RNA
TL;DR: An approach for generating fluorescent sensors based on Spinach, an RNA sequence that binds and activates the fluorescence of a small-molecule fluorophore is described, showing that these sensors can detect a variety of different small molecules in vitro and in living cells.
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Metabolite Measurement: Pitfalls to Avoid and Practices to Follow
TL;DR: The common pitfalls encountered in metabolomics are reviewed and concrete guidelines for obtaining accurate metabolite measurements are provided, focusing on water-soluble primary metabolites.
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Visualizing RNA dynamics in live cells with bright and stable fluorescent RNAs.
Xianjun Chen,Xianjun Chen,Zhang Dasheng,Ni Su,Bao Bingkun,Xin Xie,Fangting Zuo,Yang Lipeng,Hui Wang,Li Jiang,Qiuning Lin,Mengyue Fang,Li Ningfeng,Hua Xin,Chen Zhengda,Chunyan Bao,Jinjin Xu,Wenli Du,Lixin Zhang,Yuzheng Zhao,Linyong Zhu,Joseph Loscalzo,Yi Yang,Yi Yang +23 more
TL;DR: Peppers, a series of monomeric, bright and stable FRs with a broad range of emission maxima spanning from cyan to red, are developed and it is believed these FRs will be useful tools for live imaging of cellular RNAs.
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Engineered riboswitches: Expanding researchers' toolbox with synthetic RNA regulators.
Alexander Wittmann,Beatrix Suess +1 more
TL;DR: An overview of engineered riboswitches is presented and their application in conditional gene expression systems is discussed and structural and mechanistic insights are provided and problems and recent trends in the development are pointed out.
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Riboswitches and Translation Control
TL;DR: In this review, the mechanisms of riboswitch-mediated translation control are summarized to highlight both their diversity and potential ancient origins.