Tailoring the porosity and pore size of electrospun synthetic human elastin scaffolds for dermal tissue engineering.
TL;DR: Low and high porosity synthetic human elastin scaffolds obtained by adapting low and high flow rates respectively during electrospinning presented increased fiber diameter and greater average pore size and over doubling of overall scaffold porosity.
About: This article is published in Biomaterials.The article was published on 2011-10-01. It has received 256 citations till now. The article focuses on the topics: Scaffold.
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TL;DR: The ability of pore size and porosity of scaffolds to direct cellular responses and alter the mechanical properties of scaffold will be reviewed, followed by a look at nature's own scaffold, the extracellular matrix.
Abstract: Tissue engineering applications commonly encompass the use of three-dimensional (3D) scaffolds to provide a suitable microenvironment for the incorporation of cells or growth factors to regenerate ...
2,075 citations
Cites background from "Tailoring the porosity and pore siz..."
...culture.(139) Thus, the role of porosity and interconnectivity in scaffolds is also to facilitate cell migration within the porous structure such that cell growth is enabled while overcrowding is avoided....
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...5% and mean pore size of 8mm only promoted cell proliferation across the scaffold surface.(139) The fibroblasts cultured in these SHE scaffolds with higher porosity and larger mean pore sizes also deposited fibronectin and collagen type I over the period of cell...
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01 Jan 2016
TL;DR: Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells as discussed by the authors, and the potential uses of the system as an immu- nologically tolerated "tissue" for wound hea ing and as a model for studying fibroblast function are discussed.
Abstract: Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the con- centration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immu- nologically tolerated "tissue" for wound hea ing and as a model for studying fibroblast function are discussed.
1,837 citations
TL;DR: An exploration of the structural, mechanical, biochemical and biological information present in native human tissue for bioengineering applications is focused on to provide inspiration for the design of future biomaterials.
754 citations
TL;DR: An overview of the limitations of electrospinning as a tissue‐engineered scaffolding technique is provided, with emphasis on possible resolutions of those issues.
Abstract: Tissue engineering holds great promise to develop functional constructs resembling the structural organization of native tissues to improve or replace biological functions, with the ultimate goal of avoiding organ transplantation. In tissue engineering, cells are often seeded into artificial structures capable of supporting three-dimensional (3D) tissue formation. An optimal scaffold for tissue-engineering applications should mimic the mechanical and functional properties of the extracellular matrix (ECM) of those tissues to be regenerated. Amongst the various scaffolding techniques, electrospinning is an outstanding one which is capable of producing non-woven fibrous structures with dimensional constituents similar to those of ECM fibres. In recent years, electrospinning has gained widespread interest as a potential tissue-engineering scaffolding technique and has been discussed in detail in many studies. So why this review? Apart from their clear advantages and extensive use, electrospun scaffolds encounter some practical limitations, such as scarce cell infiltration and inadequate mechanical strength for load-bearing applications. A number of solutions have been offered by different research groups to overcome the above-mentioned limitations. In this review, we provide an overview of the limitations of electrospinning as a tissue-engineered scaffolding technique, with emphasis on possible resolutions of those issues. Copyright © 2015 John Wiley & Sons, Ltd.
402 citations
Cites background or methods from "Tailoring the porosity and pore siz..."
...In this regard, Rnjak-Kovacina et al. (2011) used a high injection flow rate to obtain a high-porosity elastin scaffold....
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...Crosslinking can be performed either by heat, such as dehydrothermal treatment (Drexler and Powell, 2011), radiation, including ultraviolet or γ-irradiation (Zhou et al., 2012), or chemical agents, including glutaraldehyde (Rnjak-Kovacina et al., 2011)....
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TL;DR: Current strategies to develop advanced nan ofibrous polymer-based scaffolds via electrospinning, their applications in regenerating human musculoskeletal tissues, and the use of polymer nanofibers to deliver growth factors or small molecules for regenerative medicine are summarized.
381 citations
References
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TL;DR: Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells.
Abstract: Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the concentration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immunologically tolerated "tissue" for wound healing and as a model for studying fibroblast function are discussed.
1,883 citations
"Tailoring the porosity and pore siz..." refers background in this paper
...Collagen-based scaffolds currently dominate the dermal substitute field but are limited by lack of elasticity, substantial scaffold contraction and scar formation during the repair process [3,4]....
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01 Jan 2016
TL;DR: Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells as discussed by the authors, and the potential uses of the system as an immu- nologically tolerated "tissue" for wound hea ing and as a model for studying fibroblast function are discussed.
Abstract: Fibroblasts can condense a hydrated collagen lattice to a tissue-like structure 1/28th the area of the starting gel in 24 hr. The rate of the process can be regulated by varying the protein content of the lattice, the cell number, or the con- centration of an inhibitor such as Colcemid. Fibroblasts of high population doubling level propagated in vitro, which have left the cell cycle, can carry out the contraction at least as efficiently as cycling cells. The potential uses of the system as an immu- nologically tolerated "tissue" for wound hea ing and as a model for studying fibroblast function are discussed.
1,837 citations
TL;DR: The near-comprehensive range of import filters that allow easy access to image and meta-data, a broad suite processing and analysis routine, and enthusiastic support from a friendly mailing list are invaluable for all microscopy labs and facilities-not just those on a budget.
Abstract: of short add-on programs to provide additional functionality to the core program. These additional files are either written in Java (the plugins) or in ImageJ’s macro programming language (macros). Once saved to the ImageJ plugins folder, these functions are loaded on start-up and can be accessed via menu commands like any other core function. 400+ PLUGINS
1,775 citations
"Tailoring the porosity and pore siz..." refers methods in this paper
...ASW acknowledges grant support from the Australian Research Council, the National Health and Medical Research Council" National Health and Medical Research Council and the Defence Health Foundation....
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...The widths of 10 fibers within an image for a total of 10 images per sample (n 1⁄4 3 per treatment) were quantified using ImageJ (National Institutes of Health) [31]....
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...The widths of 10 fibers within an image for a total of 10 images per sample (n ¼ 3 per treatment) were quantified using ImageJ (National Institutes of Health) [31]....
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TL;DR: The scaffold design presented in this study allows for cellular infiltration into the scaffolds while at the same time providing nanofibers as a physical mimicry of extracellular matrix.
918 citations
TL;DR: Electrostatic fiber formation, also known as "electrospinning", has emerged in recent years as the popular choice for producing continuous threads, fiber arrays and nonwoven fabrics with fiber diameters below 1 microm for a wide range of materials.
584 citations