Targeted gene expression as a means of altering cell fates and generating dominant phenotypes.
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Cites background or methods from "Targeted gene expression as a means..."
...This work was supported by an NIH grant (R01NotI from pGaTN (Brand and Perrimon, 1993) and inserted into the NS36623) to L. L. pCaSpeR4 carrying the tubulin 1a promoter. mCD8-GFP in pUAST Received October 13, 1998; revised December 11, 1998....
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...We also generated a tubP-GAL4 transgene thatexpression system in flies (Brand and Perrimon, 1993)....
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...Finally, mCD8-GFP was subcloned into pUAST (Brand and Perrimon, 1993) with XhoI and XbaI as the cloning sites....
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...…potently inhibitedreporter genes under the control of the GAL4 upstream activation sequence (UAS) in yeast (Giniger et al., 1985) GAL4-induced marker expression in embryos (Figures 2G and 2H), third instar larval brain (data not shown)and flies (Fischer et al., 1988; Brand and Perrimon, 1993)....
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Cites methods from "Targeted gene expression as a means..."
...We use a bipartite expression system that relies on transcriptional activation by the yeast protein GAL4 (ref...
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References
3,102 citations
"Targeted gene expression as a means..." refers methods in this paper
...Flies were raised on standard Drosophila medium at 25°C. Descriptions of balancers and mutations that are not described in the text can be found in Lindsley and Zimm (1992)....
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"Targeted gene expression as a means..." refers background in this paper
...The GAL4 protein is a potent transcriptional activator in yeast, and the protein has been extensively characterized with respect to both DNA binding and transcriptional activation (reviewed by Ptashne, 1988)....
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1,343 citations
"Targeted gene expression as a means..." refers background or methods in this paper
...Transgenic lines were generated by injection of CsCl banded DNA, at a concentration of 600 µg/ml, into embryos of strain y w; +/+; Sb, P[ry+, ∆2-3]/TM6, Ubx (Robertson et al., 1988) using standard procedures (Santamaria, 1986; Spradling, 1986)....
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...In addition, the enhancer detection/GAL4 vector can be mobilized to new genomic sites simply by P-transposition (Cooley et al., 1988; Robertson et al., 1988)....
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...The P-element transposons were mobilized using the ‘jumpstarter’ strain P[ry+;∆2-3], which carries a defective P-element on the third chromosome at 99B (Laski et al., 1986; Robertson et al., 1988; Cooley et al., 1988)....
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...…within an essential gene on the X-chromosome (GAL4-lethal) was used as a starter line to mobilize pGawB to different sites in the genome by introduction of a constitutively active Ptransposase gene (∆2-3; Laski et al., 1986; Robertson et al., 1988; Cooley et al., 1988; see Materials and methods)....
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1,214 citations