Figure. 1. CD4+ T cells with high affinity TCRs undergo enhanced accumulation in lymphoid and non-lymphoid organs following mycobacterial infection. (A) Schematic diagram illustrating the adoptive transfer and infection model. Magnetically-purified CD4+ T cells from C7xGFPxRag1—/— or C24xGFPxRag1—/— TCR transgenic (Tg) mice were transferred i.v. into separate intact, naïve C57BL/6 (B6) recipients (105 / mouse) 1 d prior to i.v. inoculation with BCG-E6 (106 CFU / mouse). The liver-draining lymph node (dLN), spleen, liver and spleen was collected at various time-points p.i.. (B) Flow cytometric detection of GFP-expressing TCR Tg T cells in the spleens of recipient mice that did not receive or received transgenic T cells 3 days after BCG-E6 infection. (C) Flow cytometric analysis of CTV dilution in GFP-expressing CD4+ C7 and C24 cells in the dLN, liver and spleen at day 1, 2, 3 and 6 p.i.. Black and red FACS plots represent C7 and C24 CD4+ T cells, respectively. (D) Frequency of GFP+ C7 or C24 cells in the dLN, liver and spleen at the indicated time-points. Data are pooled from three independent experiments with similar trends; Data shown are the mean percentage ± sem (n > 8 mice / group / time point). Symbols or lines in black and red represent C7 and C24 cells, respectively. Statistical differences between C7 and C24 cells were determined by Student’s t-test (*p<0.05, **p < 0.01).
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