Testicular delta5-3beta-hydroxysteroid dehydrogenase, ascorbic acid and dehydroascorbic acid in actinomycin D-treated toads.
TL;DR: Unilaterally intratesticular injection of actionomycin D in toad inhibited Δ5-3β dehydrogenase activity with significant increase of dehydroascorbic acid and decrease of ascorbic acid level in the testis.
Abstract: Unilaterally intratesticular injection of actionomycin D in toad inhibited Δ5-3β dehydrogenase activity with significant increase of dehydroascorbic acid and decrease of ascorbic acid level in the testis.
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TL;DR: A model recently proposed for the reaction of actinomycin with DNA and to the implications of this model for the template function of helical nucleic acids for the role of RNA metabolism in many systems is related particularly to this discussion.
Abstract: Publisher Summary The chapter discusses the current understanding of actinomycin action at the molecular level. This discussion is related particularly to a model recently proposed for the reaction of actinomycin with DNA and to the implications of this model for the template function of helical nucleic acids. Actinomycin blocks the expression of genetic potentialities in several ways. The most prominent of these involves suppression of the formation of the primary cellular gene products; the ribonucleic acids (RNAs). The antibiotic also affects gene action, by preventing the transport of gene products, from nucleus to cytoplasm. In some mammalian cells, exposure to actinomycin is associated with the breakdown of previously synthesized RNA and this may constitute a third form of interference, with the gene action, although such depolymerization of RNA may merely reflect the existence of a normal turnover cycle. The actinomycin has also been applied to the analysis of gene action and the role of RNA metabolism in many systems. The chapter also reviews briefly some of these areas. It has not been attempted to include all the relevant contributions, but was tried to cite representative studies that illustrate the nature of actinomycin action, within a particular experimental framework, or exemplify some current applications of the antibiotic to various biological problems and the kind of information it can help to obtain.
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