Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul (Anura: Leptodactylidae: Leiuperinae) with activity against Escherichia coli.

TL;DR: Patagonia's biodiversity has been explored from many points of view, however, skin secretions of native amphibians have not been evaluated for antimicrobial peptide research until now and the first peptides described for amphibians of the Pleurodema genus are described.

About: This article is published in Gene.The article was published on 2017-03-20 and is currently open access. It has received 21 citations till now. The article focuses on the topics: Antimicrobial peptides & Pleurodema.

Summary

Introduction

• The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form.
• B IPCSH – CONICET, Consejo Nacional de Investigaciones Científicas y Técnicas, Bvd. Brown 2915.

INTRODUCCTION

• During the last decades there has been a rapid increase in the number of antimicrobial peptides (AMPs) described, with more than 1017 active peptides derived from amphibians (antimicrobial peptide database, APD).
• Figure 1. (A) Pleurodema thaul distribution from the International Union for Conservation of Nature (IUCN).
• (B) Collection site area (photo by S. Polcowñuk, used with permission).

RESULTS AND DISCUSSION

• Preproregions (signal peptide and acidic region boxed white and black respectively) and variable domain (boxed gray) that correspond to mature peptide are AC CE PT ED M AN US CR IP T signaled.
• It is noteworthy that the highest similarity belongs to membrane proteins described in Gram-negative microorganisms.
• Synthetic purified and quantified thaulin-1 and Gly-thaulin- 1 peptides showed identical minimal inhibitory concentration (MIC) and minimal bactericidal concentration (MBC) values (Table 4), demonstrating that the mechanism of action is mainly bactericide, not involving a significant inhibitory stage.

CONCLUSIONS

• This work reports the first four peptides identified from the skin of the Patagonian frog P. thaul.
• Sequences were analyzed using the Lasergene sequence analysis software (DNASTAR, Inc.).
• All tests were performed in triplicate and according to CLSI (2012).
• Cultures of BMDM were supplemented with the different concentrations of the test compounds for 24h.
• Peptides were tested at different concentrations (15.62–500 µg/mL) according to Bignami [Bignami, 1993] with modifications.

Frequently asked questions

Q1. What contributions have the authors mentioned in the paper "Thaulin-1: the first antimicrobial peptide isolated from the skin of a patagonian frog pleurodema thaul (anura: leptodactylidae: leiuperinae) with activity against escherichia coli" ?

Please cite this article as: Mariela M. Marani, Luis O. Perez, Alyne Rodrigues de Araujo, Alexandra Plácido, Carla F. Sousa, Patrick Veras Quelemes, Mayara Oliveira, Ana G. Gomes-Alves, Mariana Pueta, Paula Gameiro, Ana M. Tomás, Cristina Delerue-Matos, Peter Eaton, Silvia A. Camperi, Néstor G. Basso, Jose Roberto de Souza de Almeida Leite, Thaulin-1: The first antimicrobial peptide isolated from the skin of a Patagonian frog Pleurodema thaul ( Anura: Leptodactylidae: Leiuperinae ) with activity against Escherichia coli. The address for the corresponding author was captured as affiliation for all authors. 

Q2. What have the authors stated for future works in "Thaulin-1: the first antimicrobial peptide isolated from the skin of a patagonian frog pleurodema thaul (anura: leptodactylidae: leiuperinae) with activity against escherichia coli" ?

Further work will be performed to explore this application.