The AmMYB308 and AmMYB330 transcription factors from antirrhinum regulate phenylpropanoid and lignin biosynthesis in transgenic tobacco
Lodovico Tamagnone,Ángel Mérida,Adrian J. Parr,Steve Mackay,Francisco A. Culianez-Macia,Keith Roberts,Cathie Martin +6 more
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TLDR
It is demonstrated that overexpression of two MYB genes from Antirrhinum represses phenolic acid metabolism and lignin biosynthesis in transgenic tobacco plants.Abstract:
MYB-related transcription factors are known to regulate different branches of flavonoid metabolism in plants and are believed to play wider roles in the regulation of phenylpropanoid metabolism in general. Here, we demonstrate that overexpression of two MYB genes from Antirrhinum represses phenolic acid metabolism and lignin biosynthesis in transgenic tobacco plants. The inhibition of this branch of phenylpropanoid metabolism appears to be specific to AmMYB308 and AmMYB330, suggesting that they recognize their normal target genes in these transgenic plants. Experiments with yeast indicate that AmMYB308 can act as a very weak transcriptional activator so that overexpression may competitively inhibit the activity of stronger activators recognizing the same target motifs. The effects of the transcription factors on inhibition of phenolic acid metabolism resulted in complex modifications of the growth and development of the transgenic plants. The inhibition of monolignol production resulted in plants with at least 17% less lignin in their vascular tissue. This reduction is of importance when designing strategies for the genetic modification of woody crops.read more
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The phenylpropanoid pathway and plant defence—a genomics perspective
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TL;DR: The availability of the complete genome sequence of Arabidopsis thaliana, and the extensive expressed sequence tag resources in other species allow, for the first time, a full appreciation of the comparative genetic complexity of the phenylpropanoid pathway across species.
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The myb transcription factor superfamily of arabidopsis: expression analysis and phylogenetic comparison with the rice myb family
Chen Yanhui,Yang Xiaoyuan,He Kun,Liu Mei-hua,Li Jigang,Gao Zhaofeng,Lin Zhiqiang,Zhang Yun-fei,Wang Xiaoxiao,Qiu Xiaoming,Shen Yunping,Shen Yunping,Zhang Li,Deng Xiaohui,Luo Jing-chu,Deng Xing-Wang,Deng Xing-Wang,Chen Zhang-liang,Gu Hongya,Qu Li-jia +19 more
TL;DR: This analysis indicated that the expression for most of the Arabidopsis MYB genes were responsive to one or multiple types of hormone and stress treatments, and may help elucidate the possible biological roles of the MyB genes in various aspects of flowering plants.
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Phenols in the plant and in man. The potential for possible nutritional enhancement of the diet by modifying the phenols content or profile
Adrian J. Parr,G.P. Bolwell +1 more
TL;DR: Information provided provides a basis for attempts to modify and optimise the phenolic content of food crops, using either conventional plant breeding along with manipulation of agronomic practices, or else the more targeted approaches of modern molecular biology.
Journal ArticleDOI
Functional genomics by integrated analysis of metabolome and transcriptome of Arabidopsis plants over-expressing an MYB transcription factor.
Takayuki Tohge,Yasutaka Nishiyama,Yasutaka Nishiyama,Masami Yokota Hirai,Mitsuru Yano,Jun Ichiro Nakajima,Motoko Awazuhara,Eri Inoue,Hideki Takahashi,Dayan B. Goodenowe,Masahiko Kitayama,Masaaki Noji,Mami Yamazaki,Kazuki Saito +13 more
TL;DR: In this paper, a comprehensive analysis of the metabolome and transcriptome of Arabidopsis thaliana over-expressing the PAP1 gene encoding an MYB transcription factor was performed for the identification of novel gene functions involved in flavonoid biosynthesis.
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