Poster
NanoSpain2010 23-26 March, 2010 Malaga-Spain
The bactericidal effect of silver nanoparticles
Piksová K.
a
, Weiserová M.
b
, Jedličková A.
c
, Cieslar M..
d
and Fojtik A.
a
,
a
Department of Physical Electronics, Faculty of Nuclear Sciences and Physical Engineering,
Czech Technical University in Prague, Břehová 7,115 19 Prague 1, Czech Republic
b
Division of Cell and Molecular Microbiology, Institute of Microbiology, v.v.i.; Academy of
Sciences of the Czech Republic, Vídeňská 1083, 142 20 Prague 4, Czech Republic
c
Institute of Clinical Biochemistry and Laboratory Diagnostics, Clinical Microbiology and
ATB Centre, General Teaching Hospital, Ke Karlovu 2, 128 00 Prague 2, Czech Republic
d
Department of Physics of Materials, Faculty of Mathematics and Physics, Charles
University in Prague, Ke Karlovu 5, 128 00 Prague 2, Czech Republic
katerina.piksova@fjfi.cvut.cz
Nanotechnology is expected to open new avenues to fight and prevent diseases using atomic
scale tailoring of materials. Rapid development of bio-nanotechnology and material research
leads to a new way in combating bacteria and searching specific properties of nanomaterials.
Presently, the increased resistance of bacteria against strong antibiotics offers to nanomaterial
research a chance to help alleviating this problem.
The present work studies the bactericidal effect of silver nanoparticles in the range of 7-50 nm
on Gram-negative bacteria and Gram-positive bacteria. The colloid silver nanoparticles was
prepared by the modified Türkewitsch´s method. This colloid particles has the specific
properties which have bactericidal effect.
Prepared silver nanoparticles were characterized by localized surface plasmon resonance
(Fig. 1) and by TEM microscope (Fig. 2). Cells were grown in Lysogeny Broth (LB) medium
and plated on Luria-agar plates (LA; LB with addition of 15% of agar). The overnight
cultures were diluted in LB such that the final concentration of cells was (1-3) . 10
7
cells/mL
and used for antimicrobial assays. After overnight cultivation a number of colonies
representing the number of living (surviving) cells was calculated. The bactericidal efficacy of
Ag nanoparticles on bacteria was estimated according to the formula I.
Formula I:
Keywords: silver nanoparticles, bactericidal
References:
[1] J. Kimling, M. Maier, B. Okenve, V. Kotaidis, H. Ballot, and A. Plech: Turkevich Method
for Gold Nanoparticle Synthesis Revisited, J. Phys. Chem. B, 110 (32), 15700 -15707
[2] Appleyard, R.K., Segregation of new lysogenic types during growth of doubly lysogenic
strains derived from Escherichia coli K-12. Genetics, 1954. 39: p. 440-452.
alive number in reference group _ alive number in experiment group
bactericidal efficacy (%)= --------------------------------------------------------------------------------------------- x 100%
alive number in reference group
Poster
NanoSpain2010 23-26 March, 2010 Malaga-Spain
[3] Luria, S.E., Host-induced modifications of viruses. Cold Spring Harb Symp Quant Biol,
1953. 18: p. 237-44.
[4] Huang, L., et al., Controllable preparation of Nano-MgO and investigation of its
bactericidal properties, J. Inorg. Biochem, 2005, 99(5):p. 986-93
[5] A. Fojtik, P. Mulvaney, T. Linnert, M. Giersig and A. Henglein, Formation and Reduction
of Semiconductor-Like Aggregates of Silver-Carboxy-Alkane-Thiolates in Aqueous
Solutions, Ber. Bunsenges. Phys. Chem. 95, 1991
[6] A. Henglein, T. Linnert, P. Mulvaney, Reduction of Ag+ in Aqueous Polyanion Solution:
Some Properties and Reactions of Long-Lived Oligomeric Silver Clusters and Metallic
Silver Particles, Ber. Bunsenges. Phys. Chem. 94, 1990
Figures:
0
0,2
0,4
0,6
0,8
1
1,2
1,4
1,6
1,8
300 350 400 450 500 550 600
wavelength[nm]
absorbance
Fig. 1: Absorption spectra of silver nanoparticles
Fig. 2: TEM characterization of silver nanoparticles