The binding of kinetin to plant ribosomes.
Reads0
Chats0
TLDR
The synthetic cytokinins kinetin and 6-benzylaminopurine exhibit equilibrium-type binding to purified chinese-cabbage leaf ribosomes and a positive correlation between the extent of binding and the biological effect of various cytokinin analogues was demonstrated.Abstract:
The synthetic cytokinins kinetin and 6-benzylaminopurine exhibit equilibrium-type binding to purified chinese-cabbage leaf ribosomes. At 23mum and 4 degrees C one molecule of kinetin and 1.34 molecules of 6-benzylaminopurine are bound per ribosome. Adenine and adenine derivatives that are inactive as cytokinins showed much less affinity for ribosomes. Pretreatment of ribosomes with 0.5m-ammonium chloride or Triton X-100 did not decrease the extent of cytokinin binding. Binding appeared to be to the 83S ribosome species. A positive correlation between the extent of binding and the biological effect of various cytokinin analogues was demonstrated. These results are discussed in terms of cytokinin control of growth processes at the ribosomal level.read more
Citations
More filters
Journal ArticleDOI
The Role of Phytohormones (Cytokinins) in Chloroplast Development
TL;DR: Although indications are presented that the promotion of chloroplast development by cytokinins is closely connected with a stimulation of the gene expression program for plastogenesis, other sites of hormone action cannot be excluded and are discussed in the last part of the review.
Journal ArticleDOI
A cytokinin binding protein from higher plant ribosomes.
J.E. Fox,J.L. Erion +1 more
TL;DR: Two types of cytokinin binding sites exist on higher plant ribosomes and one of these, a high affinity site, binds cytokinins at low concentrations, is saturated at one cytokinIn molecule per ribosome, is specific for substances with cytokin in activity.
Journal ArticleDOI
Regulation of Ribonucleic Acid Metabolism by Plant Hormones
TL;DR: This paper aims to clarify the role of receptors and mediators in the regulation of RNA metabolism in vivo and to describe the mechanisms leading to cell reprograming and subsequent down-regulation.
Book ChapterDOI
Molecular Biology of Wound Healing: The Conditioning Phenomenon
TL;DR: This chapter discusses the molecular biology of wound healing, which leads to a transient disorganization of the membrane systems of the cell that induces the switching on of genes and the synthesis of various enzymes.
Journal ArticleDOI
Structure‐activity relationships of cytokinins
TL;DR: In this article, the structure-activity relationship of cytokinins is investigated and the authors present a review of the results of their work. Critical Reviews in Plant Sciences: Vol. 9, No. 1, pp. 17-57.
References
More filters
Journal ArticleDOI
Amber suppression: a nucleotide change in the anticodon of a tyrosine transfer RNA.
TL;DR: In certain mutants a single base change alters the meaning of a messenger codon in such a way that, instead of spelling out an amino-acid, it spells out chain termination.
Journal ArticleDOI
Hormonal Regulation of Growth and Protein Synthesis
TL;DR: A topographical segregation of polyribosomes by attachment to cytoplasmic membranes, which are generated in parallel with the additional ribosomes, may facilitate the synthesis of hormone-specific proteins.
Journal ArticleDOI
The sequence of phenylalanine tRNA from E. coli
B.G. Barrell,Frederick Sanger +1 more
TL;DR: The sequence of this tRNA is studied but certain results are in conflict with the above sequence but the sequences of the nucleotides are tentatively identified as 2-thiomethyl&isopentenyl adenylic acid.
Journal ArticleDOI
Dissociation and reassociation of skeletal muscle ribosomes.
TL;DR: It is postulated that the nascent protein and perhaps messenger RNA protect the ribosome from dissociation by high concentrations of potassium.
Journal ArticleDOI
Structure of a Mammalian Serine tRNA
TL;DR: This work has compared the structure of a yeast transfer RNA with that of a mammalian one and found that yeast tyrosine tRNA lies between the dihydrouridine-containing and anticodon loops.
Related Papers (5)
In-vitro auxin binding to particulate cell fractions from corn coleoptiles.
Cytokinin-induced chlorophyll formation in cucumber cotyledons.
R. A. Fletcher,Dianne McCullagh +1 more