Journal ArticleDOI
The code and beyond: transcription regulation by the RNA polymerase II carboxy-terminal domain
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TLDR
The intricacies of the CTD code is discussed and how the newly characterized physicochemical properties ofThe CTD expand the function of theCTD beyond the code.Abstract:
The carboxy-terminal domain (CTD) extends from the largest subunit of RNA polymerase II (Pol II) as a long, repetitive and largely unstructured polypeptide chain. Throughout the transcription process, the CTD is dynamically modified by post-translational modifications, many of which facilitate or hinder the recruitment of key regulatory factors of Pol II that collectively constitute the 'CTD code'. Recent studies have revealed how the physicochemical properties of the CTD promote phase separation in the presence of other low-complexity domains. Here, we discuss the intricacies of the CTD code and how the newly characterized physicochemical properties of the CTD expand the function of the CTD beyond the code.read more
Citations
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Journal ArticleDOI
Organizational principles of 3D genome architecture
TL;DR: Observations suggest that the 3D organization of the genome is an emergent property of chromatin and its components, and thus may not be only a determinant but also a consequence of its function.
Journal ArticleDOI
The molecular language of membraneless organelles
Edward Gomes,James Shorter +1 more
TL;DR: An overview of the molecular underpinnings of the formation and regulation of these membraneless organelles are provided and new light on neurodegenerative diseases is shone on.
Journal ArticleDOI
Pol II phosphorylation regulates a switch between transcriptional and splicing condensates
Yang Eric Guo,John C. Manteiga,Jonathan E. Henninger,Benjamin R. Sabari,Alessandra Dall’Agnese,Nancy M. Hannett,Jan-Hendrik Spille,Jan-Hendrik Spille,Lena K. Afeyan,Alicia V. Zamudio,Krishna Shrinivas,Brian J. Abraham,Brian J. Abraham,Ann Boija,Tim-Michael Decker,Jenna K. Rimel,Charli B. Fant,Tong Ihn Lee,Ibrahim I Cisse,Phillip A. Sharp,Dylan J. Taatjes,Richard A. Young +21 more
TL;DR: In this paper, the authors investigated whether the phosphorylation of the C-terminal domain of the RNA polymerase II (PolII) C-interaction subunit regulates the incorporation of Pol II into phase-separated condensates that are associated with transcription initiation and splicing.
Journal ArticleDOI
Phase-separation mechanism for C-terminal hyperphosphorylation of RNA polymerase II.
Huasong Lu,Huasong Lu,Dan Yu,Anders S. Hansen,Sourav Ganguly,Rongdiao Liu,Alec Heckert,Xavier Darzacq,Qiang Zhou +8 more
TL;DR: The histidine-rich domain of cyclin T1 promotes phase separation into liquid droplets, which facilitates the hyperphosphorylation of the C-terminal domain repeats of RNA polymerase II.
Journal ArticleDOI
Born to run: control of transcription elongation by RNA polymerase II
TL;DR: The features, establishment and maintenance of Pol II pausing, the transition into productive elongation, the control of transcription elongation by enhancers and by factors of other cellular processes, such as topoisomerases and poly(ADP-ribose) polymerases (PARPs), and the potential of therapeutic targeting of the elongation stage of transcription by Pol II are discussed.
References
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Journal ArticleDOI
Widespread transcription at neuronal activity-regulated enhancers
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