The combination matters--distinct impact of lifestyle factors on sperm quality: a study on semen analysis of 1683 patients according to MSOME criteria.
TL;DR: Combinations of adverse lifestyle factors could have a detrimental impact on sperm, not only in terms of motility and sperm count but also in Terms of sperm head vacuolization.
Abstract: Background
Poor sperm quality can negatively affect embryonic development and IVF outcome. This study is aimed at investigating the influence of various lifestyle factors on semen quality according to MSOME (motile sperm organelle morphology examination) criteria.
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TL;DR: It is suggested that greater focus on collection of DNA fragmentation and progressive motility in a clinical setting may lead to better patient outcomes during fertility treatments of aging couples, even though sperm concentration did not decline with increasing male age.
257 citations
TL;DR: Results showed that alcohol intake has a detrimental effect on semen volume and normal morphology, and studies evaluating the effect of changes on semen parameters on the reproductive outcomes are needed in advance of providing recommendations regarding alcohol intake other than the advice to avoid heavy alcohol drinking.
Abstract: Alcohol consumption is widespread in the Western world. Some studies have suggested a negative association between alcohol intake and semen quality although others have not confirmed this. MEDLINE and Embase were searched using ‘alcohol intake' OR ‘alcohol consumption' OR ‘alcohol drinking' OR ‘lifestyle' combined with ‘semen quality' OR ‘sperm quality' OR ‘sperm volume' OR ‘sperm concentration' OR ‘sperm motility' for full-length observational articles, published in English. Reference lists of retrieved articles were searched for other pertinent studies. Main outcome measures were sperm parameters, if provided as means (standard deviation or standard error) or as medians (interquartile range). Fifteen cross-sectional studies were included, with 16,395 men enrolled. Main results showed that alcohol intake has a detrimental effect on semen volume (pooled estimate for no/low alcohol consumption 0.25 ml, 95% CI, 0.07 to 0.42) and normal morphology (1.87%, 95% CI, 0.86 to 2.88%). The difference was more marked when comparing occasional versus daily consumers, rather than never versus occasional, suggesting a moderate consumption did not adversely affect semen parameters. Hence, studies evaluating the effect of changes on semen parameters on the reproductive outcomes are needed in advance of providing recommendations regarding alcohol intake other than the advice to avoid heavy alcohol drinking.
125 citations
TL;DR: Severe male factor impairs early embryonic competence in terms of fertilization rate and developmental potential, however, the euploidy rate and implantation potential of the obtained blastocysts are independent from sperm quality.
96 citations
TL;DR: The literature suggests that caffeine intake, possibly through sperm DNA damage, may negatively affect male reproductive function, and well-designed studies are essential to reach a consistent evidence on the effect of caffeine on semen parameters and male fertility.
Abstract: Semen quality, a predictor of male fertility, has been suggested declining worldwide. Among other life style factors, male coffee/caffeine consumption was hypothesized to influence semen parameters, but also sperm DNA integrity. To summarize available evidence, we performed a systematic review of observational studies on the relation between coffee/caffeine intake and parameters of male fertility including sperm ploidy, sperm DNA integrity, semen quality and time to pregnancy. A systematic literature search was performed up to November 2016 (MEDLINE and EMBASE). We included all observational papers that reported the relation between male coffee/caffeine intake and reproductive outcomes: 1. semen parameters, 2. sperm DNA characteristics, 3. fecundability. All pertinent reports were retrieved and the relative reference lists were systematically searched in order to identify any potential additional studies that could be included. We retrieved 28 papers reporting observational information on coffee/caffeine intake and reproductive outcomes. Overall, they included 19,967 men. 1. Semen parameters did not seem affected by caffeine intake, at least caffeine from coffee, tea and cocoa drinks, in most studies. Conversely, other contributions suggested a negative effect of cola-containing beverages and caffeine-containing soft drinks on semen volume, count and concentration. 2. As regards sperm DNA defects, caffeine intake seemed associated with aneuploidy and DNA breaks, but not with other markers of DNA damage. 3. Finally, male coffee drinking was associated to prolonged time to pregnancy in some, but not all, studies. The literature suggests that caffeine intake, possibly through sperm DNA damage, may negatively affect male reproductive function. Evidence from epidemiological studies on semen parameters and fertility is however inconsistent and inconclusive. Well-designed studies with predefined criteria for semen analysis, subject selection, and life style habits definition, are essential to reach a consistent evidence on the effect of caffeine on semen parameters and male fertility.
90 citations
Cites background from "The combination matters--distinct i..."
...Wogatzky, 2012 [39] Mean (SD) Mean (SD) Mean (SD) Mean (SD)...
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...Wogatzky, 2012 [39] Not estimated Cups of coffee per day 204 men out of 1321 drinking coffee had an intake of more than 3 cups of coffee per day....
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...Wogatzky, 2012 [39] Austria 1683 Cross-sectional Fertility clinic: infertile couples Semen variables 40....
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TL;DR: Smoking, coffee/cola/fried foods consumption, and the effects of environmental/sociopsychobehavioral factors act on semen quality are found to be significantly associated with semen quality from the baseline investigation.
61 citations
Cites result from "The combination matters--distinct i..."
...But in other studies, decreased semen quality or its tendency was also observed.(55,56,63) Similar result was found for cola consumption in this study, but lower semen volume and lower total sperm count were found in cola consumers....
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References
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TL;DR: The present results support the use of high-magnification sperm selection for intracytoplasmic sperm injection (ICSI) using sperm nuclear vacuoles evaluated more precisely at high magnifications using motile sperm organelle morphology examination (MSOME).
Abstract: Summary
The aim of this investigation was to determine the presence of abnormal sperm chromatin packaging in spermatozoa with large nuclear vacuoles (LNV) selected via high magnification by analysing the pattern of chromomycin A3 (CMA3) staining. A prospective observational study was designed to analyse semen samples obtained from 66 men undergoing infertility diagnosis and treatment. The numbers of cells with normal (dull yellow staining of the sperm head/CMA3-negative) and abnormal (bright yellow fluorescence of the sperm head/CMA3-positive) chromatin packaging were determined on slides with normal and LNV spermatozoa. The presence of bright yellow fluorescence (CMA3-positive) was significantly higher (p < 0.0001) in spermatozoa with LNV than in normal spermatozoa (719/1351; 53.2% vs. 337/835; 40.3%, respectively), reflecting a higher percentage of abnormal chromatin packaging in spermatozoa with large LNV. Our data support the hypothesis that the presence of LNV reflects the presence of abnormal chromatin packaging, which may facilitate sperm DNA damage. As sperm nuclear vacuoles are evaluated more precisely at high magnifications using motile sperm organelle morphology examination (MSOME), the present results support the use of high-magnification sperm selection for intracytoplasmic sperm injection (ICSI).
127 citations
"The combination matters--distinct i..." refers background in this paper
...Nevertheless, several recent studies demonstrated that nuclear vacuoles were correlated to DNA damage and/or failures in chromatin packaging [20-23]....
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...Even though the origin of vacuoles is still not completely understood and it has been fiercely discussed whether those vacuoles originate from a natural process or, more likely, from pathological (stress) situations during spermatogenesis [21,22], a study by Brassesco and colleagues demonstrated that sperm vacuolization improves after antioxidant therapy [46]....
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TL;DR: The pooled data of IMSI cycles demonstrate a statisticallysignificant improvement in implantation and pregnancy rates and a statistically significant reduction in miscarriage rates, however, more randomized controlled trials are needed to confirm these results.
Abstract: The development of a modified intracytoplasmic sperm injection (ICSI), called intracytoplasmic morphologically selected sperm injection (IMSI), demonstrated that a profound morphological investigation of the spermatozoon, under the magnification of 6600×, enables outcome improvement. The aim of this study was to compare ICSI outcome with IMSI outcome. The meta-analysis results demonstrated no significant difference in fertilization rate between ICSI and IMSI groups. However, a significantly improved implantation (odds ratio (OR) 2.72; 95% confidence interval (CI) 1.50–4.95) and pregnancy rate (OR 3.12; 95% CI 1.55–6.26) was observed in IMSI cycles. Moreover, the results showed a significantly decreased miscarriage rate (OR 0.42; 95% CI 0.23–0.78) in IMSI cycles as compared with ICSI cycles. This is the first meta-analysis of published data to evaluate the potential benefits of IMSI. The pooled data of IMSI cycles demonstrate a statistically significant improvement in implantation and pregnancy rates and a statistically significant reduction in miscarriage rates. However, more randomized controlled trials are needed to confirm these results. The development of a modified intracytoplasmic sperm injection (ICSI) method, called intracytoplasmic morphologically selected sperm injection (IMSI), has enabled the improvement of pregnancy rates. The aim of this study was to perform a systematic review of the literature and compare the fertilization, implantation, pregnancy and miscarriage rates in ICSI and IMSI cycles. Our meta-analysis results demonstrated no significant difference in fertilization between ICSI and IMSI groups. However, implantation and pregnancy rates were almost 3-fold higher in IMSI cycles. Moreover, the miscarriage rate was more than 40% lower in IMSI cycles as compared with ICSI cycles. This meta-analysis demonstrates a significant improvement in implantation and pregnancy rates, and a significant reduction in miscarriage rates. However, further studies are needed to confirm our results.
125 citations
"The combination matters--distinct i..." refers methods in this paper
...The application of the MSOME-approach in ART-cycles has demonstrated to significantly improve implantation and pregnancy rates and to reduce the miscarriage rates [10,12-18]....
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TL;DR: Data show that spermatozoa selected by PGC present an improved morphology which is linked to improvement of the quality of the in-vitro fertilized embryos and ultimately the percentage of successful IVF results.
Abstract: Many groups currently use two methods for the separation of motile spermatozoa, swim-up (S-up) and centrifugation on discontinuous Percoll gradient (PGC), and comparison of results indicates that PGC is superior. In this study we have attempted to identify the factors explaining this difference. This laboratory has long-standing expertise in seminology, thus the parameters of sperm morphology were the obvious first choice for detailed study. First, the respective effects of S-up and PGC on sperm morphology were analysed in different types of ejaculates: 62 semen samples with normal parameters and 41 with poor parameters. Both separation techniques resulted in improved morphology in the final preparation but only the increase of morphologically normal spermatozoa in the final Percoll suspension was significant. Second, application of these techniques in our in-vitro fertilization (IVF) programme revealed that, together with the improvement of sperm morphology, a higher pregnancy rate was obtained after PGC. The ongoing pregnancy rates per oocyte retrieval were 21.1% for the S-up technique and 33.3% for the PGC technique. These data show that spermatozoa selected by PGC present an improved morphology which we believe to be linked to improvement of the quality of the in-vitro fertilized embryos and ultimately the percentage of successful IVF results.
123 citations
"The combination matters--distinct i..." refers background in this paper
...Sperm chromatin packaging and DNA integrity are crucial for good sperm quality as DNA damage has been found to impair reproductive outcomes due to the late paternal effect [24,25]....
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TL;DR: The influence of the male partner's lifestyle, including eating and social habits, on semen quality and intracytoplasmic sperm injection (ICSI) success was investigated to investigate the influence of both the male and female lifestyle on treatment success.
123 citations
"The combination matters--distinct i..." refers background in this paper
...Quality and quantity of food intake is supposed to influence semen parameters [1]....
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...Several publications indicate adverse effects of smoking on sperm parameters [1,37-39]....
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...Among the individual factors which are considered to increase or decrease sperm quality in humans as defined by the Kruger or WHO (World Health Organization) criteria [1-5] are environment, occupation, nutrition, stimulants, ejaculation frequency and lifestyle choices....
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TL;DR: Wang et al. as mentioned in this paper examined whether reproductive hormones play a role in the association between body mass index (BMI) and semen quality and found that low BMI was associated with reduced semen quality.
Abstract: Aim: To examine whether reproductive hormones play a role in the association between body mass index (BMI) and semen quality. Methods: Semen quality and testosterone (T), luteinizing hormone (LH), follicle-stimulating hormone (FSH) and estradiol (E2) were evaluated in 990 fertile males with age 38.9 ± 9.7 (mean ± SD) years recruited from the Chinese general population in 2001 and 2002. Results: Semen quality was reduced among underweight (BMI < 18.5) compared with normal (BMI 18.5–24.9) and overweight (BMI 25.0–29.9), but the associations were independent of reproductive hormones. After adjustment for the potential confounders, underweight men had reductions in sperm concentration (22.4 × 10 6 /mL), total sperm count (52.9 × 10 6 ) and percentage of normal sperm forms (6.9%) compared with men with normal BMI. Being underweight may be a risk factor for low sperm concentration (OR: 4.68, 95% confidence intervals [CI]: 2.01–10.91). Otherwise, being overweight may be a protected factor for low sperm concentration (OR: 0.25; 95% CI: 0.08–0.83) and low total sperm count (OR: 0.37, 95% CI: 0.15–0.87). Conclusion: Low BMI was associated with reduced semen quality. The associations between BMI and semen quality were found statistically significant even after adjustment for reproductive hormones. Reproductive hormones cannot explain the association between BMI and semen quality. (Asian J Androl 2007 Nov; 9: 827–834)
110 citations