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Journal ArticleDOI

The determination of ascorbic acid in whole blood and urine through the 2,4-dinitrophenylhydrazine derivative of dehydroascorbic acid

01 Feb 1943-Journal of Biological Chemistry (American Society for Biochemistry and Molecular Biology)-Vol. 147, Iss: 2, pp 399-407
About: This article is published in Journal of Biological Chemistry.The article was published on 1943-02-01 and is currently open access. It has received 1689 citations till now. The article focuses on the topics: Ascorbic acid & Dehydroascorbic acid.
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Book ChapterDOI
TL;DR: This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids and suggests that prompt stabilization is especially important in the case of plasma or serum.
Abstract: Publisher Summary This chapter discusses selected methods for the determination of ascorbic acid in animal cells, tissues, and fluids. Methods for determining ascorbic acid are numerous. In general, chemical analyses for the vitamin are divided into two groups; the determination of the reduced form and the determination of the oxidized form. The former group of analyses is usually based upon the oxidation–reduction properties of ascorbic acid. These are widely used as the fundamental reactions in the measurement of vitamin C. The latter group of analyses is usually based upon the oxidation of the ascorbic acid and the subsequent formation of a hydrazone or a fluorophore. Best results are obtained if samples, especially plasma, are quickly stabilized with either trichloroacetic acid or metaphosphoric acid and immediately analyzed. Prompt stabilization is especially important in the case of plasma or serum. The greater stability of ascorbic acid in acid solution is because of the decreased tendency for the hydrolysis of the lactone ring with decreasing pH.

1,843 citations

Journal ArticleDOI
TL;DR: Some pharmacological actions of curcumin (diferuloyl methane) have been examined in rats, mice and cats and it prevents the inflammation induced increase in SGOT and SGPT levels.
Abstract: Some pharmacological actions of curcumin (diferuloyl methane) have been examined in rats, mice and cats. The compound possesses significant anti-inflammatory activity in acute as well as in chronic models of inflammation. It is as potent as phenylbutazone in the carrageenan oedema test but only half as potent in chronic tests. Curcumin possesses a much lower ulcerogenic index than phenylbutazone. It prevents the inflammation induced increase in SGOT and SGPT levels. It lacks analgesic and antipyretic activity. It has no other significant pharmacological effects. The oral LD50 in mice is more than 2m0 g kg−1.

658 citations

Journal ArticleDOI
TL;DR: The data obtained in the present study suggests that the extracts of Moringa oleifera both mature and tender leaves have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage.
Abstract: Antioxidants play an important role in inhibiting and scavenging free radicals, thus providing protection to human against infections and degenerative diseases. Current research is now directed towards natural antioxidants originated from plants due to safe therapeutics. Moringa oleifera is used in Indian traditional medicine for a wide range of various ailments. To understand the mechanism of pharmacological actions, antioxidant properties of the Moringa oleifera leaf extracts were tested in two stages of maturity using standard in vitro models. The successive aqueous extract of Moringa oleifera exhibited strong scavenging effect on 2, 2-diphenyl-2-picryl hydrazyl (DPPH) free radical, superoxide, nitric oxide radical and inhibition of lipid per oxidation. The free radical scavenging effect of Moringa oleifera leaf extract was comparable with that of the reference antioxidants. The data obtained in the present study suggests that the extracts of Moringa oleifera both mature and tender leaves have potent antioxidant activity against free radicals, prevent oxidative damage to major biomolecules and afford significant protection against oxidative damage.

535 citations

Journal ArticleDOI
TL;DR: A new colorimetric technique for the estimation of ascorbic acid by using Folin phenol reagent has been developed and has been found to be stable up to 18 h and almost 100% efficient.

475 citations

Journal ArticleDOI
01 Sep 1973
TL;DR: The CRC Critical Reviews in Clinical Laboratory Sciences: Vol. 4, No. 3, No 3, pp 215-340 as discussed by the authors was the first publication of a comprehensive review of laboratory tests for the assessment of nutritional status.
Abstract: (1973). Laboratory Tests for the Assessment of Nutritional Status. CRC Critical Reviews in Clinical Laboratory Sciences: Vol. 4, No. 3, pp. 215-340.

458 citations