The Determination of Enzyme Dissociation Constants
01 Mar 1934-Journal of the American Chemical Society (American Chemical Society)-Vol. 56, Iss: 3, pp 658-666
TL;DR: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, where (E:l = (ES).
Abstract: On the basis of the assumed theory the rate of the observed reaction is directly proportional to the concentration of the enzyme-substrate compound, (ES), a t all values of the concentration of the substrate, (S). It is proportional to (S) only a t low values of (S). The numerical value of the dissociation constant is given by the substrate concentration a t half-maximum velocity, where (E:l = (ES). The equilibrium in equation 1 may be heterogeneous or homogeneous. Hitchcock'\" has pointed
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Cites background from "The Determination of Enzyme Dissoci..."
...Note that competitiveness can be determined by the Lineweaver-Burk plot (Lineweaver and Burk, 1934) plot, whereas exclusiveness can be determined by the median-effect (Chou, 1976) plot....
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...Competitiveness can be determined by the Lineweaver-Burk plot (Lineweaver and Burk, 1934), and exclusivity can be determined by the median-effect plot (Chou, 1976)....
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