The determination of hydroxyproline.
01 May 1950-Journal of Biological Chemistry (American Society for Biochemistry and Molecular Biology)-Vol. 184, Iss: 1, pp 299-306
TL;DR: A short and simple calorimetric method is here described that is applicable to the determination of hydroxyproline in hydrolysates of 40 to 100 y of collagen with a reproducibility of f2 per cent and an accuracy of f1 per cent as judged by recovery of hydroXYproline from elastin hydrolysate and from an amino acid mixture simulating collagen.
About: This article is published in Journal of Biological Chemistry.The article was published on 1950-05-01 and is currently open access. It has received 1249 citations till now. The article focuses on the topics: Tryptophan & Amino acid.
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TL;DR: Method has been applied to a study of hydroxyproline distribution in cell particulates, tissue fluids, and purified plant and animal proteins, and significant amounts of hydroXYproline were found in crystalline preparations of pepsin, elastase.
3,756 citations
TL;DR: A modified ninhydrin reagent for the photometric determination of amino acids and related compounds and its application in drug discovery is described.
2,851 citations
TL;DR: A critical review is given of hydroxyproline analysis, finding a procedure which is faster and more sensitive than the former one and as reliable.
1,731 citations
1,515 citations
TL;DR: The model suggests that lipid plays an essential role and show that even substances which are not intrinsically surface active can have profound effects when the composition of the interfacial phase is suitably chosen, and further studies of its changes of physical state under narcotics may be very informative.
Abstract: ing from this point of view, one could say that we have built and tested a crude membrane model in which such a phenomenon might be observed, and that thus far the binding energies agree satisfactorily with those calculated from in vivo experiments only when the model contains lipid. In so far as we understand the behavior of the model, it does not permit us to state whether the interactions occur primarily between lipid film and narcotic or between water and narcotic, facilitated by the presence of lipid. In either interpretation, our data suggest that lipid plays an essential role and show that even substances which are not intrinsically surface active can have profound effects when the composition of the interfacial phase is suitably chosen.\"7 The sensitivity of the model to inert gases and the quantitative similarity of its reactions to their biological effect suggest that further studies of its changes of physical state under narcotics may be very informative.
1,234 citations
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128 citations
TL;DR: Very little work has been done upon its chemical and physical characterization since the early work of Richards and Gies (1) in their study of ligament, of Abderhalden and Schittenhelm (2) who accounted for 61 per cent by weight of the amino acids in elastin, and of Kossel and Kutscher (3) who studied the basic amino acid content as mentioned in this paper.
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