The distribution of dust mite allergen in the houses of patients with asthma.
01 Nov 1981-The American review of respiratory disease (Am Rev Respir Dis)-Vol. 124, Iss: 5, pp 630-635
TL;DR: The results suggested that natural exposure to this dust allergen allows occasional fecal particles to enter the lungs and that these particles contain very concentrated allerGEN.
Abstract: Using an inhibition radioimmunoassay for the major allergen from Dermatophagoides pteronyssinus (antigen P1), we studied the distribution of this dust allergen in the houses of patients with asthma. Both bed and floor dust samples contained a wide range of antigen P1, 100 to 100,000 ng/g of fine dust, and this concentration correlated well with the number of mite bodies (r = 0.81, p less than 0.001). We were unable to detect antigen P1 in the air of undisturbed rooms. However, during domestic activity, between 1 and 30 ng were collected on a filter than sampled air for 45 min at 17 L/min. Using a cascade impactor it was shown that greater than 80% of the airborne antigen P1 was associated with particles greater than 10 mu in diameter. Some of the particles containing allergen could be identified because they formed precipitin rings when impacted onto agarose containing rabbit antimite antiserum. These particles had the physical appearance of mite feces, which are the major source of antigen P1 in mite cultures. The results suggested that natural exposure to this dust allergen allows occasional fecal particles to enter the lungs and that these particles contain very concentrated allergen.
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Journal Article•
841 citations
TL;DR: Measurements from a radioimmunoassay show that more than 95% of the allergen accumulating in mite cultures is associated with faecal particles.
Abstract: The association between house dust allergy and asthma has long been recognized, and it has been demonstrated that a major allergen in house dust is related to the presence of mites of the genus Dermatophagoides Using extracts of mite culture for skin testing, as many as 10% of the population and up to 90% of allergic asthmatics give positive immediate reactions Although mites may occasionally become airborne during bed-making, it has also been demonstrated that they 'secrete or excrete' some allergen Recently, we have shown that up to three-quarters of the serum IgE antibodies to mites are directed against a major allergen-antigen P1 (molecular weight 24,000) Using a radioimmunoassay it is possible to measure the concentration of this glycoprotein in both dust samples and mite cultures These measurements, which are reported here, show that more than 95% of the allergen accumulating in mite cultures is associated with faecal particles
508 citations
TL;DR: The finding of the house-dust mite seems to supply an answer to all factors, and the keystone of the theory is formed by the fact that after being made equivalent, extracts of house dust and mite cultures gave skin reactions which were both qualitatively and quantitatively indistinguishable.
417 citations
TL;DR: The mites in a large number of dust samples from houses of patients with bronchial asthma were identified and counted: the most common species was Dermatophagoides pteronyssinus, and it was particularly abundant in mattress dust.
267 citations
TL;DR: Mite counts and tests for mite antigen were performed on samples of dust taken from the bedding of 53 children with mite-sensitive asthma and no significant differences emerged in the progress of the two groups, both tending to improve.
Abstract: Mite counts and tests for mite antigen were performed on samples of dust taken from the bedding of 53 children with mite-sensitive asthma. The samples from damp houses and the beds or enuretic children had markedly more mites and mite-antigen than those from dry houses. although the predominant species was usually Dermatophagoides pteronyssinus, some of the beds in the damp houses were heavily infested with another pyroglyphid mite Euroglyphus maynei, so that this was the species found in the greatest numbers. D pteronyssinus antigen was found to be correlated broadly with the total mite count, but more antigen was present for a given number of mites in the mattresses than in the blankets. The children were randomly allocated into two groups, one of which carried out rigorous anti-mite measures. The amounts of dust and mite antigen were reduced, though not the numbers of mites. Peak flow readings were monitored in the two groups for eight weeks and a final assessment made by a paediatrician who was unaware of the allocation of each patient in the trial. No significant differences emerged in the progress of the two groups, both tending to improve. Measures designed to remove mites from bedding do not greatly benefit the majority of children with mite-sensitive asthma.
149 citations