The draft genome of sweet orange (Citrus sinensis).
Qiang Xu,Ling-Ling Chen,Xiaoan Ruan,Dijun Chen,Andan Zhu,Chunli Chen,Denis Bertrand,Wen-Biao Jiao,Bao Hai Hao,Matthew P. Lyon,Jiongjiong Chen,Song Gao,Feng Xing,Hong Lan,Ji Wei Chang,Xianhong Ge,Yang Lei,Qun Hu,Yin Miao,Lun Wang,Shi-Xin Xiao,Manosh Kumar Biswas,Wenfang Zeng,Fei Guo,Hongbo Cao,Xiaoming Yang,Xi Wen Xu,Yun Jiang Cheng,Juan Xu,Ji-Hong Liu,Oscar Junhong Luo,Zhonghui Tang,Wen-Wu Guo,Hanhui Kuang,Hong-Yu Zhang,Mikeal L. Roose,Niranjan Nagarajan,Xiuxin Deng,Yijun Ruan,Yijun Ruan +39 more
Reads0
Chats0
TLDR
Focused analysis on genes involved in vitamin C metabolism showed that GalUR, encoding the rate-limiting enzyme of the galacturonate pathway, is significantly upregulated in orange fruit, and the recent expansion of this gene family may provide a genomic basis.Abstract:
Oranges are an important nutritional source for human health and have immense economic value Here we present a comprehensive analysis of the draft genome of sweet orange (Citrus sinensis) The assembled sequence covers 873% of the estimated orange genome, which is relatively compact, as 20% is composed of repetitive elements We predicted 29,445 protein-coding genes, half of which are in the heterozygous state With additional sequencing of two more citrus species and comparative analyses of seven citrus genomes, we present evidence to suggest that sweet orange originated from a backcross hybrid between pummelo and mandarin Focused analysis on genes involved in vitamin C metabolism showed that GalUR, encoding the rate-limiting enzyme of the galacturonate pathway, is significantly upregulated in orange fruit, and the recent expansion of this gene family may provide a genomic basis This draft genome represents a valuable resource for understanding and improving many important citrus traits in the futureread more
Citations
More filters
Automated Eukaryotic Gene Structure Annotation Using EVidenceModeler and the Program to Assemble Spliced Alignments
Brian J. Haas,Steven L. Salzberg,Wei Zhu,Mihaela Pertea,Jonathan E. Allen,Joshua Orvis,Owen White,C R Buell,Jennifer R. Wortman +8 more
TL;DR: The experiments on both rice and human genome sequences demonstrate that EVM produces automated gene structure annotation approaching the quality of manual curation.
Journal ArticleDOI
Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species
Seungill Kim,Minkyu Park,Seon-In Yeom,Yong-Min Kim,Je Min Lee,Hyun-Ah Lee,Eunyoung Seo,Jaeyoung Choi,Kyeongchae Cheong,Ki-Tae Kim,Kyongyong Jung,Gir-Won Lee,Sang-Keun Oh,Chungyun Bae,Saet-Byul Kim,Hyeyoung Lee,Shin Young Kim,Myung-Shin Kim,Byoung-Cheorl Kang,Yeong Deuk Jo,Hee Bum Yang,Hee-Jin Jeong,Won-Hee Kang,Jin Kyung Kwon,Chanseok Shin,Jae Yun Lim,June Hyun Park,Jin Hoe Huh,June Sik Kim,Byung-Dong Kim,Oded Cohen,Ilan Paran,Mi Chung Suh,Saet Buyl Lee,Yeon Ki Kim,Younhee Shin,Seung-Jae Noh,Junhyung Park,Young Sam Seo,Suk-Yoon Kwon,Hyun A Kim,Jeong Mee Park,Hyunjin Kim,Sang Bong Choi,Paul W. Bosland,Gregory Reeves,Sung Hwan Jo,Bong Woo Lee,Hyung-Taeg Cho,Hee-Seung Choi,Min-Soo Lee,Yeisoo Yu,Yang Do Choi,Beom Seok Park,Allen Van Deynze,Hamid Ashrafi,Theresa Hill,Woo Taek Kim,Hyun Sook Pai,Hee-Kyung Ahn,Inhwa Yeam,James J. Giovannoni,James J. Giovannoni,Jocelyn K. C. Rose,Iben Sørensen,Sang Jik Lee,Ryan W. Kim,Ik-Young Choi,Beom-Soon Choi,Jong-Sung Lim,Yong-Hwan Lee,Doil Choi +71 more
TL;DR: The genome size of the hot pepper was approximately fourfold larger than that of its close relative tomato, and the genome showed an accumulation of Gypsy and Caulimoviridae family elements.
Journal ArticleDOI
Genome sequencing and population genomics in non-model organisms.
TL;DR: High-throughput sequencing technologies are revolutionizing the life sciences, and the past 12 months have seen a burst of genome sequences from non-model organisms, in each case representing a fundamental source of data of significant importance to biological research.
Journal ArticleDOI
Sequencing of diverse mandarin, pummelo and orange genomes reveals complex history of admixture during citrus domestication
G Albert Wu,Simon E. Prochnik,Jerry Jenkins,Jérôme Salse,Uffe Hellsten,Florent Murat,Xavier Perrier,Manuel Ruiz,Simone Scalabrin,Javier Terol,Marco Aurélio Takita,Karine Labadie,Julie Poulain,Arnaud Couloux,Kamel Jabbari,Federica Cattonaro,Cristian Del Fabbro,Sara Pinosio,Andrea Zuccolo,Jarrod Chapman,Jane Grimwood,Francisco R. Tadeo,Leandro H. Estornell,Juan V. Munoz-Sanz,Victoria Ibanez,Amparo Herrero-Ortega,Pablo Aleza,Julián Pérez-Pérez,Daniel Ramón,Dominique Brunel,François Luro,Chunxian Chen,William G. Farmerie,Brian Desany,Chinnappa D. Kodira,Mohammed Mohiuddin,Tim Harkins,Karin M. Fredrikson,Paul Burns,Alexandre Lomsadze,Mark Borodovsky,Giuseppe Reforgiato,Juliana Freitas-Astúa,Francis Quetier,Luis Navarro,Mikeal L. Roose,Patrick Wincker,Jeremy Schmutz,Michele Morgante,Marcos A. Machado,Manuel Talon,Olivier Jaillon,Patrick Ollitrault,Frederick G. Gmitter,Daniel S. Rokhsar +54 more
TL;DR: This work sequence and compare citrus genomes—a high-quality reference haploid clementine genome and mandarin, pummelo, sweet-orange and sour-orange genomes—and shows that cultivated types derive from two progenitor species.
Journal ArticleDOI
The coffee genome provides insight into the convergent evolution of caffeine biosynthesis
Lorenzo Carretero-Paulet,Alexis Dereeper,Gaëtan Droc,Romain Guyot,Marco Pietrella,Chunfang Zheng,Adriana Alberti,François Anthony,G. Aprea,Jean-Marc Aury,Pascal Bento,Maria Bernard,Stéphanie Bocs,Claudine Campa,Alberto Cenci,Alberto Cenci,Marie Christine Combes,Dominique Crouzillat,Corinne Da Silva,Loretta Daddiego,Fabien De Bellis,Stéphane Dussert,Olivier Garsmeur,Thomas Gayraud,Valentin Guignon,Katharina Jahn,Katharina Jahn,Véronique Jamilloux,Thierry Joët,Karine Labadie,Tianying Lan,Tianying Lan,Julie Leclercq,Maud Lepelley,Thierry Leroy,Leiting Li,Pablo Librado,Loredana Lopez,Adriana Muñoz,Adriana Muñoz,Benjamin Noel,Alberto Pallavicini,Gaetano Perrotta,Valérie Poncet,David Pot,Priyono,Michel Rigoreau,Mathieu Rouard,Julio Rozas,Christine Tranchant-Dubreuil,Robert VanBuren,Qiong Zhang,Alan Carvalho Andrade,Xavier Argout,Benoît Bertrand,Alexandre de Kochko,Giorgio Graziosi,Giorgio Graziosi,Robert J Henry,Jayarama,Ray Ming,Chifumi Nagai,Steve Rounsley,David Sankoff,Giovanni Giuliano,Victor A. Albert,Patrick Wincker,Patrick Wincker,Patrick Wincker,Philippe Lashermes +69 more
TL;DR: The Coffea canephora (coffee) genome was sequenced and identified a conserved gene order, and comparative analyses of caffeine NMTs demonstrate that these genes expanded through sequential tandem duplications independently of genes from cacao and tea, suggesting that caffeine in eudicots is of polyphyletic origin.
References
More filters
Journal ArticleDOI
The Sequence Alignment/Map format and SAMtools
Heng Li,Bob Handsaker,Alec Wysoker,T. J. Fennell,Jue Ruan,Nils Homer,Gabor T. Marth,Gonçalo R. Abecasis,Richard Durbin +8 more
TL;DR: SAMtools as discussed by the authors implements various utilities for post-processing alignments in the SAM format, such as indexing, variant caller and alignment viewer, and thus provides universal tools for processing read alignments.
Journal ArticleDOI
Fast and accurate short read alignment with Burrows–Wheeler transform
Heng Li,Richard Durbin +1 more
TL;DR: Burrows-Wheeler Alignment tool (BWA) is implemented, a new read alignment package that is based on backward search with Burrows–Wheeler Transform (BWT), to efficiently align short sequencing reads against a large reference sequence such as the human genome, allowing mismatches and gaps.
Journal ArticleDOI
MUSCLE: multiple sequence alignment with high accuracy and high throughput
TL;DR: MUSCLE is a new computer program for creating multiple alignments of protein sequences that includes fast distance estimation using kmer counting, progressive alignment using a new profile function the authors call the log-expectation score, and refinement using tree-dependent restricted partitioning.
Journal ArticleDOI
The Genome Analysis Toolkit: A MapReduce framework for analyzing next-generation DNA sequencing data
Aaron McKenna,Matthew Hanna,Eric Banks,Andrey Sivachenko,Kristian Cibulskis,Andrew Kernytsky,Kiran V. Garimella,David Altshuler,Stacey Gabriel,Mark J. Daly,Mark A. DePristo +10 more
TL;DR: The GATK programming framework enables developers and analysts to quickly and easily write efficient and robust NGS tools, many of which have already been incorporated into large-scale sequencing projects like the 1000 Genomes Project and The Cancer Genome Atlas.
Journal ArticleDOI
Ultrafast and memory-efficient alignment of short DNA sequences to the human genome
TL;DR: Bowtie extends previous Burrows-Wheeler techniques with a novel quality-aware backtracking algorithm that permits mismatches and can be used simultaneously to achieve even greater alignment speeds.
Related Papers (5)
The grapevine genome sequence suggests ancestral hexaploidization in major angiosperm phyla.
Olivier Jaillon,Jean-Marc Aury,Benjamin Noel,Alberto Policriti,Christian Clepet,Alberto Casagrande,Nathalie Choisne,Sébastien Aubourg,Nicola Vitulo,Claire Jubin,Alessandro Vezzi,Fabrice Legeai,Philippe Hugueney,Corinne Dasilva,David S. Horner,Erica Mica,Delphine Jublot,Julie Poulain,Clémence Bruyère,Alain Billault,Béatrice Segurens,Michel Gouyvenoux,Edgardo Ugarte,Federica Cattonaro,Véronique Anthouard,Virginie Vico,Cristian Del Fabbro,Michael Alaux,Gabriele Di Gaspero,Vincent Dumas,Nicoletta Felice,Sophie Paillard,Irena Juman,Marco Moroldo,Simone Scalabrin,Aurélie Canaguier,Isabelle Le Clainche,G Malacrida,Eléonore Durand,Graziano Pesole,Valérie Laucou,Philippe Chatelet,Didier Merdinoglu,Massimo Delledonne,Mario Pezzotti,Alain Lecharny,Claude Scarpelli,François Artiguenave,M. Enrico Pè,Giorgio Valle,Michele Morgante,Michel Caboche,Anne-Françoise Adam-Blondon,Jean Weissenbach,Francis Quetier,Patrick Wincker +55 more
The genome of the domesticated apple ( Malus × domestica Borkh.)
Riccardo Velasco,Andrey Zharkikh,Jason P. Affourtit,Amit Dhingra,Alessandro Cestaro,Ananth Kalyanaraman,Paolo Fontana,Satish Bhatnagar,Michela Troggio,Dmitry Pruss,Silvio Salvi,Massimo Pindo,Paolo Baldi,Sara Castelletti,Marina Cavaiuolo,G. Coppola,Fabrizio Costa,V. Cova,Antonio Dal Ri,Vadim V. Goremykin,M. Komjanc,Sara Longhi,P. Magnago,Giulia Malacarne,Mickael Malnoy,Diego Micheletti,Marco Moretto,Michele Perazzolli,Azeddine Si-Ammour,Silvia Vezzulli,E. Zini,Glenn Eldredge,Lisa M. Fitzgerald,N. Gutin,Jerry S. Lanchbury,Teresita Macalma,J.T. Mitchell,Julia Reid,Bryan Wardell,Chinnappa D. Kodira,Zhoutao Chen,Brian Desany,Faheem Niazi,Melinda Palmer,Tyson Koepke,Derick Jiwan,Scott Schaeffer,Vandhana Krishnan,Changjun Wu,Vu T. Chu,Stephen T. King,Jessica Vick,Quanzhou Tao,Amy Mraz,Aimee Stormo,Keith E. Stormo,Robert Bogden,Davide Ederle,Alessandra Stella,Alberto Vecchietti,Martin M. Kater,Simona Masiero,Pauline Lasserre,Yves Lespinasse,Andrew C. Allan,Vincent G. M. Bus,David Chagné,Ross N. Crowhurst,Andrew P. Gleave,Enrico Lavezzo,Jeffrey A. Fawcett,Jeffrey A. Fawcett,Sebastian Proost,Sebastian Proost,Pierre Rouzé,Pierre Rouzé,Lieven Sterck,Lieven Sterck,Stefano Toppo,Barbara Lazzari,Roger P. Hellens,Charles-Eric Durel,Alexander Gutin,Roger E. Bumgarner,Susan E. Gardiner,Mark H. Skolnick,Michael Egholm,Yves Van de Peer,Yves Van de Peer,Francesco Salamini,Roberto Viola +90 more
Analysis of relative gene expression data using real-time quantitative pcr and the 2(-delta delta c(t)) method
The genome of black cottonwood, Populus trichocarpa (Torr. & Gray)
Gerald A. Tuskan,Gerald A. Tuskan,Stephen P. DiFazio,Stephen P. DiFazio,Stefan Jansson,Joerg Bohlmann,Igor V. Grigoriev,Uffe Hellsten,Nicholas H. Putnam,Steven G. Ralph,Stephane Rombauts,Asaf Salamov,Jacquie Schein,Lieven Sterck,Andrea Aerts,Rishikeshi Bhalerao,Rishikesh P. Bhalerao,Damien Blaudez,Wout Boerjan,Annick Brun,Amy M. Brunner,Victor Busov,Malcolm M. Campbell,John E. Carlson,Michel Chalot,Jarrod Chapman,G.-L. Chen,Dawn Cooper,Pedro M. Coutinho,Jérémy Couturier,Sarah F. Covert,Quentin C. B. Cronk,R. Cunningham,John M. Davis,Sven Degroeve,Annabelle Déjardin,Claude W. dePamphilis,John C. Detter,Bill Dirks,Inna Dubchak,Inna Dubchak,Sébastien Duplessis,Jürgen Ehlting,Brian E. Ellis,Karla C Gendler,David Goodstein,Michael Gribskov,Jane Grimwood,Andrew Groover,Lee E. Gunter,Björn Hamberger,Berthold Heinze,Yrjö Helariutta,Yrjö Helariutta,Yrjö Helariutta,Bernard Henrissat,D. Holligan,Robert A. Holt,Wenyu Huang,N. Islam-Faridi,Steven J.M. Jones,M. Jones-Rhoades,Richard A. Jorgensen,Chandrashekhar P. Joshi,Jaakko Kangasjärvi,Jan Karlsson,Colin T. Kelleher,Robert Kirkpatrick,Matias Kirst,Annegret Kohler,Udaya C. Kalluri,Frank W. Larimer,Jim Leebens-Mack,Jean-Charles Leplé,Philip F. LoCascio,Y. Lou,Susan Lucas,Francis Martin,Barbara Montanini,Carolyn A. Napoli,David R. Nelson,C D Nelson,Kaisa Nieminen,Ove Nilsson,V. Pereda,Gary F. Peter,Ryan N. Philippe,Gilles Pilate,Alexander Poliakov,J. Razumovskaya,Paul G. Richardson,Cécile Rinaldi,Kermit Ritland,Pierre Rouzé,D. Ryaboy,Jeremy Schmutz,J. Schrader,Bo Segerman,H. Shin,Asim Siddiqui,Fredrik Sterky,Astrid Terry,Chung-Jui Tsai,Edward C. Uberbacher,Per Unneberg,Jorma Vahala,Kerr Wall,Susan R. Wessler,Guojun Yang,T. Yin,Carl J. Douglas,Marco A. Marra,Göran Sandberg,Y. Van de Peer,Daniel S. Rokhsar,Daniel S. Rokhsar +115 more
The tomato genome sequence provides insights into fleshy fruit evolution
Shusei Sato,Satoshi Tabata,Hideki Hirakawa,Erika Asamizu,Kenta Shirasawa,Sachiko Isobe,Takakazu Kaneko,Yasukazu Nakamura,Daisuke Shibata,Koh Aoki,Michael Egholm,James R. Knight,Robert Bogden,Changbao Li,Yang Shuang,Xun Xu,Shengkai Pan,Shifeng Cheng,Xin Liu,Yuanyuan Ren,Jun Wang,Alessandro Albiero,Francesca Dal Pero,Sara Todesco,Joyce Van Eck,Robert M. Buels,Aureliano Bombarely,Joseph Gosselin,Minyun Huang,Jonathan A. Leto,Naama Menda,Susan R. Strickler,Linyong Mao,Shan Gao,Isaak Y. Tecle,Thomas L. York,Yi Zheng,Julia Vrebalov,Je Min Lee,Silin Zhong,Lukas A. Mueller,Willem J. Stiekema,Paolo Ribeca,Tyler Alioto,Wencai Yang,Sanwen Huang,Yongchen Du,Zhonghua Zhang,Jianchang Gao,Yanmei Guo,Xiaoxuan Wang,Ying Li,Jun He,Chuanyou Li,Zhukuan Cheng,Jianru Zuo,Jianfeng Ren,Jiuhai Zhao,Liuhua Yan,Hongling Jiang,Bao Wang,Hongshuang Li,Zhenjun Li,Fuyou Fu,Bingtang Chen,Bin Han,Qi Feng,Danlin Fan,Ying Wang,Hong-Qing Ling,Yongbiao Xue,Doreen Ware,W. Richard McCombie,Zachary B. Lippman,Jer Ming Chia,Ke Jiang,Shiran Pasternak,Laura Gelley,Melissa Kramer,Lorinda K. Anderson,Song Bin Chang,Suzanne M. Royer,Lindsay A. Shearer,Stephen M. Stack,Jocelyn K. C. Rose,Yimin Xu,Nancy T. Eannetta,Antonio J. Matas,Ryan P. McQuinn,Steven D. Tanksley,Francisco Camara,Roderic Guigó,Stephane Rombauts,Jeffrey A. Fawcett,Yves Van de Peer,Dani Zamir,Chunbo Liang,Manuel Spannagl,Heidrun Gundlach,Rémy Bruggmann,Klaus F. X. Mayer,Zhiqi Jia,Junhong Zhang,Zhibiao Ye,Gerard J. Bishop,Sarah Butcher,Rosa Lopez-Cobollo,Daniel W. A. Buchan,Ioannis Filippis,James Abbott,Manju Singh,Alok Singh,Jitendra Kumar Pal,Awadhesh Pandit,Pradeep Kumar Singh,Ajay Kumar Mahato,Vivek Dogra,Kishor Gaikwad,Tilak Raj Sharma,Trilochan Mohapatra,Nagendra K. Singh,Mathilde Causse,Christophe Rothan,Céline Noirot,Arnaud Bellec,Christophe Klopp,Corinne Delalande,Hélène Bergès,Jérôme Mariette,Pierre Frasse,Sonia Vautrin,Toulouse Mohamed Zouine,Alain Latché,Christine Rousseau,Farid Regad,Jean-Claude Pech,Murielle Philippot,Mondher Bouzayen,Pierre Pericard,Sonia Osorio,Asuncion Fernandez Del Carmen,Antonio J. Monforte,Antonio Granell,Rafael Fernández-Muñoz,Mariana Conte,Gabriel Lichtenstein,Fernando Carrari,Gianluca De Bellis,Fabio Fuligni,Clelia Peano,Silvana Grandillo,Pasquale Termolino,Marco Pietrella,Elio Fantini,Giulia Falcone,Alessia Fiore,Giovanni Giuliano,Loredana Lopez,Paolo Facella,Gaetano Perrotta,Loretta Daddiego,Glenn J. Bryan,Biology Modesto Orozco,Xavier Pastor,David Torrents,Marco G.M. Van Schriek,Richard Feron,Jan van Oeveren,Peter De Heer,Lorena Da Ponte,Saskia Jacobs-Oomen,Mike Cariaso,Marcel Prins,Michael Josephus Theresia Van Eijk,Antoine Janssen,J. J. Van Haaren,Sung HwanJo,Jungeun Kim,Suk-Yoon Kwon,Sangmi Kim,Dal-Hoe Koo,Sanghyeob Lee,Christopher Clouser,Alain Rico,Asis Hallab,Christiane Gebhardt,Kathrin Klee,Anika Jöcker,Jens Warfsmann,Ulrike Göbel,Shingo Kawamura,Kentaro Yano,Jamie D. Sherman,Hiroyuki Fukuoka,Satomi Negoro,Sarita Bhutty,Parul Chowdhury,Debasis Chattopadhyay,Erwin Datema,Sandra Smit,Elio Schijlen,José van de Belt,Jan C. van Haarst,Sander Peters,Marjo J. van Staveren,Marleen H.C. Henkens,Paul Mooyman,Thamara Hesselink,Roeland C. H. J. van Ham,Guoyong Jiang,Marcus Droege,Doil Choi,Byung Cheol Kang,Byung-Dong Kim,Minkyu Park,Seungill Kim,Seon-In Yeom,Yong-Hwan Lee,Yang Do Choi,Guangcun Li,Jianwei Gao,Yongsheng Liu,Shengxiong Huang,Victoria Fernandez-Pedrosa,Carmen Collado,Sheila Zuñ Iga,Guoping Wang,Rebecca Cade,Robert A. Dietrich,Jane Rogers,Sandra Knapp,Zhangjun Fei,Zhangjun Fei,Ruth White,Ruth White,Theodore W. Thannhauser,Theodore W. Thannhauser,James J. Giovannoni,James J. Giovannoni,Miguel A. Botella,Louise Gilbert,Fabra Ramon Gonzalez,Jose Luis Goicoechea,Yeisoo Yu,David Kudrna,Kristi Collura,Marina Wissotski,Rod A. Wing,Blake C. Meyers,Aishwarya Bala Gurazada,Pamela J. Green,Saloni Mathur,Shailendra Vyas,Amolkumar U. Solanke,Rajesh Kumar,Vikrant Gupta,Arun Sharma,Paramjit Khurana,Jitendra P. Khurana,Akhilesh K. Tyagi,Tamas Dalmay,Irina Mohorianu,Brandon Walts,Srikar Chamala,W. Brad Barbazuk,Jingping Li,Hui Guo,Tae-Ho Lee,Yupeng Wang,Dong Zhang,Andrew H. Paterson,Xiyin Wang,Xiyin Wang,Haibao Tang,Haibao Tang,Amalia Barone,Maria Luisa Chiusano,Maria Raffaella Ercolano,Nunzio D’Agostino,Miriam Di Filippo,Alessandra Traini,Walter Sanseverino,Luigi Frusciante,Graham B. Seymour,Mounir Elharam,Ying Fu,Axin Hua,Steven Kenton,Jennifer Lewis,Shaoping Lin,Fares Z. Najar,Hongshing Lai,Baifang Qin,Ruihua Shi,Chunmei Qu,Douglas White,James R. White,Yanbo Xing,Keqin Yang,Jing Yi,Ziyun Yao,Liping Zhou,Bruce A. Roe,Alessandro Vezzi,Michela D'Angelo,Rosanna Zimbello,Riccardo Schiavon,Elisa Caniato,Chiara Rigobello,Davide Campagna,Nicola Vitulo,Giorgio Valle,David R. Nelson,Emanuele De Paoli,Dóra Szinay,Hans H. De Jong,Yuling Bai,Richard G. F. Visser,Reném Klein Lankhorst,Helen Beasley,Karen McLaren,Christine Nicholson,Claire Riddle,Giulio Gianese +323 more